<scp>l</scp>-DNA Molecular Beacon: A Safe, Stable, and Accurate Intracellular Nano-thermometer for Temperature Sensing in Living Cells

Ke, Guoliang; Wang, Chunming; Ge, Yun; Zheng, Nanfeng; Zhu, Zhi; Yang, Chaoyong

doi:10.1021/ja3082439

Cited by 172 publications

(165 citation statements)

References 48 publications

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“…Conjugate DNAzymes with polyamines and peptides, terminal modification of N3′-P5′ phosphoramidate bonds were also reported to enhance stability 144, 145. It is also known that the L-formed DNA is more stable to resist nuclease digestion than naturally occurring D-form DNA 146. Based on this fact, Tan and coworkers constructed L-DNAzyme using L-DNA 147.…”

Section: Delivery Of Dnazymesmentioning

confidence: 99%

Theranostic DNAzymes

Zhou¹,

Ding²,

Liu³

2017

Theranostics

194

143

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DNAzymes are catalytically active DNA molecules that are obtained via in vitro selection. RNA-cleaving DNAzymes have attracted significant attention for both therapeutic and diagnostic applications due to their excellent programmability, stability, and activity. They can be designed to cleave a specific mRNA to down-regulate gene expression. At the same time, DNAzymes can sense a broad range of analytes. By combining these two functions, theranostic DNAzymes are obtained. This review summarizes the progress of DNAzyme for theranostic applications. First, in vitro selection of DNAzymes is briefly introduced, and some representative DNAzymes related to biological applications are summarized. Then, the applications of DNAzyme for RNA cleaving are reviewed. DNAzymes have been used to cleave RNA for treating various diseases, such as viral infection, cancer, inflammation and atherosclerosis. Several formulations have entered clinical trials. Next, the use of DNAzymes for detecting metal ions, small molecules and nucleic acids related to disease diagnosis is summarized. Finally, the theranostic applications of DNAzyme are reviewed. The challenges to be addressed include poor DNAzyme activity under biological conditions, mRNA accessibility, delivery, and quantification of gene expression. Possible solutions to overcome these challenges are discussed, and future directions of the field are speculated.

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Section: Delivery Of Dnazymesmentioning

confidence: 99%

Theranostic DNAzymes

Zhou¹,

Ding²,

Liu³

2017

Theranostics

194

143

View full text Add to dashboard Cite

show abstract

“…Although many nanoscale temperature measurement techniques such as scanning thermal microscopy have been developed or are under development as reviewed elsewhere [16], its measurement in biological and physiological environments has not been well established and is an area of active research. One attractive approach is based on the use of fluorescence nanomaterials [290][291][292][293] because of its compatibility with aqueous environments. In addition, it can be easily integrated with other biomedical optical techniques for noninvasive temperature measurements.…”

Section: Nanoscale and Nanoparticle Thermometrymentioning

confidence: 99%

“…Using these temperaturedependent fluorescence characteristics, a QD-mediated thermometry technique has been developed to provide intraoperative guidance of thermal surgeries [296][297][298]. In addition to surgical guidance, the thermometry has been extended to measure nanoscale as well as subcellular thermal features [290,293,299,300]. Although this technique can address many challenges of micro-and nanoscale temperature measurements for biomedical applications, further research is warranted when it combines with nanoparticle-mediated heating Downloaded by [New York University] at 07: 56 12 June 2015 modalities.…”

Section: Nanoscale and Nanoparticle Thermometrymentioning

confidence: 99%

Evaluating Broader Impacts of Nanoscale Thermal Transport Research

Shi

Dames

Lukes

et al. 2015

Nanoscale and Microscale Thermophysical Engineering

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The past two decades have witnessed the emergence and rapid growth of the research field of nanoscale thermal transport. Much of the work in this field has been fundamental studies that have explored the mechanisms of heat transport in nanoscale films, wires, particles, interfaces, and channels. However, in recent years there has been an increasing emphasis on utilizing the fundamental knowledge gained toward understanding and improving Manuscript 128 L. SHI ET AL.device and system performances. In this opinion article, an attempt is made to provide an evaluation of the existing and potential impacts of the basic research efforts in this field on the developments of the heat transfer discipline, workforce, and a number of technologies, including heat-assisted magnetic recording, phase change memories, thermal management of microelectronics, thermoelectric energy conversion, thermal energy storage, building and vehicle heating and cooling, manufacturing, and biomedical devices. The goal is to identify successful examples, significant challenges, and potential opportunities where thermal science research in nanoscale has been or will be a game changer.

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“…After NIR irradiation, cell apoptosis was induced by the heat from photothermal effect of AuNRs, and the fluorescence was further enhanced because the remaining folded MB could be unwinded into the single DNA in the condition of high temperature. 42 The further enhanced fluorescence signal defined as "enhanced on" state. Therefore, the fluorescence of the AuNR probe was a direct feedback of photothermal effect induced by the NIR irradiation and offered an efficient way for the monitoring the therapeutic efficiency in real time.…”

Section: ■ Introductionmentioning

confidence: 99%

MicroRNA-Responsive Cancer Cell Imaging and Therapy with Functionalized Gold Nanoprobe

Liu

Zhang

Lei

et al. 2015

ACS Appl. Mater. Interfaces

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Integration of cancer cell imaging and therapy is critical to enhance the theranostic efficacy and prevent under- or overtreatment. Here, a multifunctional gold nanoprobe is designed for simultaneous miRNA-responsive fluorescence imaging and therapeutic monitoring of cancer. By assembling with folic acid as the targeted moiety and a dye-labeled molecular beacon (MB) as the recognition element and signal switch, the gold nanoprobe is folate receptor-targeted delivered into the cancer cells, and the fluorescence is lighted with the unfolding of MB by intracellular microRNA (miRNA), resulting in an efficient method for imaging and detecting nucleic acid. The average quantity of miRNA-21 is measured to be 1.68 pg in a single HeLa cell. Upon the near-infrared irradiation at 808 nm, the real-time monitoring and assessing of photothermal therapeutic efficacy is achieved from the further enhanced fluorescence of the dye-labeled MB, caused by the high photothermal transformation efficiency of the gold nanocarrier to unwind the remaining folded MB and depart the dye from the nanocarrier. The fluorescence monitoring is also feasible for applications in vivo. This work provides a simple but powerful protocol with great potential in cancer imaging, therapy, and therapeutic monitoring.

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l-DNA Molecular Beacon: A Safe, Stable, and Accurate Intracellular Nano-thermometer for Temperature Sensing in Living Cells

Cited by 172 publications

References 48 publications

Theranostic DNAzymes

Theranostic DNAzymes

Evaluating Broader Impacts of Nanoscale Thermal Transport Research

MicroRNA-Responsive Cancer Cell Imaging and Therapy with Functionalized Gold Nanoprobe

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