<scp>L</scp>-Cysteine protects intestinal integrity, attenuates intestinal inflammation and oxidant stress, and modulates NF-κB and Nrf2 pathways in weaned piglets after LPS challenge

Song, Zehe; Guo, Tong; Xiao, Kan; Jiao, Lefei; Yao, Ke; Hu, Chengbao

doi:10.1177/1753425916632303

Cited by 74 publications

(66 citation statements)

References 37 publications

(71 reference statements)

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“…This raised the possibility of a complex chronology of inflammatory signaling in skeletal muscle occurring earlier in the response to environmental hyperthermia. LPS can activate inflammatory signaling (Song et al 2016) and endotoxemia appears to be a consistent effect of heat stress (Pearce et al 2012(Pearce et al , 2013a. Moreover, we found recently that 12 h of heat stress led to oxidative stress and that it has long been appreciated that oxidative stress can promote inflammatory signaling (Ambade and Mandrekar 2012).…”

Section: Introductionmentioning

confidence: 84%

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Acute heat stress activated inflammatory signaling in porcine oxidative skeletal muscle

et al. 2017

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Despite well‐studied clinical manifestations, intracellular mechanisms of prolonged hyperthermic injury remain unclear, especially in skeletal muscle. Given muscle's large potential to impact systemic inflammation and metabolism, the response of muscle cells to heat‐mediated injury warrants further investigation. We have previously reported increased activation of NF‐κB signaling and increased NF‐κB and AP‐1‐driven transcripts in oxidative skeletal muscle following 12 h of heat stress. The purpose of this investigation was to examine early heat stress‐induced inflammatory signaling in skeletal muscle. We hypothesized that heat stress would increase NF‐κB and AP‐1 signaling in oxidative skeletal muscle. To address this hypothesis, 32 gilts were randomly assigned to one of four treatment groups (n = 8/group): control (0 h: 21°C) or exposed to heat stress conditions (37°C) for 2 h (n = 8), 4 h (n = 8), or 6 h (n = 8). Immediately following environmental exposure pigs were euthanized and the red portion of the semitendinosus muscle (STR) was harvested. We found evidence of NF‐κB pathway activation as indicated by increased protein abundance of NF‐κB activator IKK‐α following 4 h and increased total NF‐κB protein abundance following 6 h of heat stress. Heat stress also stimulated AP‐1 signaling as AP‐1 protein abundance was increased in nuclear fractions following 4 h of heat stress. Interleukin‐6 protein abundance and activation of the JAK/STAT pathway were decreased in heat stressed muscle. These data indicate that heat stress activated inflammatory signaling in the porcine STR muscle via the AP‐1 pathway and early activation of the NF‐κB pathway.

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Section: Introductionmentioning

confidence: 84%

“…LPS can activate inflammatory signaling (Song et al. 2016) and endotoxemia appears to be a consistent effect of heat stress (Pearce et al. 2012, 2013a,c).…”

Section: Introductionmentioning

confidence: 97%

Acute heat stress activated inflammatory signaling in porcine oxidative skeletal muscle

et al. 2017

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“…Previous studies have shown that LPS induces acute bacterial infection, followed by stimulating macrophages and monocytes to produce and secrete related pro-inflammatory cytokines, eventually leading to organism systemic inflammation. 29 Consistent with improved intestinal integrity, dietary Cu/Zn-Mt decreased pro-inflammatory cytokine expression (TNF-a, IL-1b, IL-6, and IL-8), which indicated that LPS-induced inflammation was minimized by Cu/Zn-Mt supplementation. Similar to our findings, Namkung et al 28 reported that dietary addition of Zn and Cu could help maintain homeostasis and immune function in weaned pigs after LPS challenge.…”

Section: Discussionmentioning

confidence: 85%

Copper/zinc-loaded montmorillonite influences intestinal integrity, the expression of genes associated with inflammation, TLR4–MyD88 and TGF-β1 signaling pathways in weaned pigs after LPS challenge

Wang

Gong

et al. 2017

Innate Immun

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This study was aimed at investigating whether dietary copper/zinc-loaded montmorillonite (Cu/Zn-Mt) could alleviate Escherichia coli LPS-induced intestinal injury through pro-and anti-inflammatory signaling pathways (TLRs, NLRs and TGF-b1) in weaned piglets. Eighteen 21-d-old pigs were randomly divided into three groups (control, LPS and LPS + Cu/Zn-Mt). After 21 d of feeding, pigs in the LPS group and LPS + Cu/Zn-Mt group received i.p. administration of LPS, whereas pigs in the control group received saline. At 4 h post-injection, jejunum samples were collected for analysis. The results indicated that, compared with the LPS group, supplemental Cu/Zn-Mt increased transepithelial electrical resistance, the expressions of anti-inflammatory cytokines (TGF-b1) in mRNA and protein levels, and decreased FD4 and the mRNA expression of pro-inflammatory cytokines (TNF-a, IL-6, IL-8 and IL-1b). The pro-inflammatory signaling pathways results demonstrated that Cu/Zn-Mt supplementation decreased the mRNA levels of TLR4 and its downstream signals (MyD88, IRAK1, TRAF6) but had no effect on NOD1 and NOD2 signals. Cu/Zn-Mt supplementation did not affect NF-kB p65 mRNA abundance, but down-regulated its protein expression. The anti-inflammatory signaling pathways results showed supplemental Cu/Zn-Mt also increased TbRII, Smad4 and Smad7 mRNA expressions. These findings suggested dietary Cu/Zn-Mt attenuated LPS-induced intestinal injury by alleviating intestinal inflammation, influencing TLR4-MyD88 and TGF-b1 signaling pathways in weaned pig.

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“…In this scenario, it is plausible that l-cysteine supplementation exhibits beneficial effects on corneal integrity by preventing an excessive oxidative state due to PRK surgery and thus by reducing the epithelial prolonged inflammation it favors a faster stromal recovery. In fact, it is well established the role of cysteine in alleviating intestinal inflammation and oxidative stress through the inhibition the NF-κB and the activation of the Nrf2 signaling pathways [36], which modulates the proliferation of epithelial cell. Similarly, it is well known that the activation of NF-kB pathway is involved in corneal inflammation [37], which could justify the beneficial effects of l-cysteine on corneal regeneration.…”

Section: Discussionmentioning

confidence: 99%

Oral l-Cysteine Supplementation Enhances the Long Term-Effect of Topical Basic Fibroblast Growth Factor (bFGF) in Reducing the Corneal Haze after Photorefractive Keratectomy in Myopic Patients

et al. 2020

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We aimed at evaluating the long-term effects of l-cysteine oral supplementation to basic fibroblast growth factor (bFGF) eye-drops on corneal re-epithelization and transparency in myopic patients subjected to photorefractive keratectomy (PRK). Forty patients subjected to bilateral PRK for myopia were enrolled and randomly divided into two groups receiving an additional therapy together with the standard postoperative treatment consisting in local tobramycin 0.3%, dexamethasone 0.1%, diclofenac 0.1%, and 0.2% hyaluronate. Group 1 included 20 patients (11 males and 9 females; 34.09 ± 8 years of age) receiving only bFGF eye-drops (10 μg/10 μL) four times a day for 7 days starting from the day of surgery; Group 2 included 20 patients (12 males and 8 females; 37.35 ± 11.5 years of age) who were postoperatively administered with topical basic fibroblast growth factor (bFGF; 10 μg/10 μL) four times a day for 7 days plus oral l-cysteine supplementation (500 mg/capsule) once a day for 15 days, starting 7 days before PRK. Patients were followed-up for 12 months. Clinical ophthalmologic parameters were recorded for all the 80 examined eyes. The corneal transparency was evaluated in vivo by slit lamp and confocal microscopy. The data showed that: (a) the corneal haze occurred in a smaller percentage of the patients who were postoperatively administered with topical bFGF plus oral l-cysteine supplementation (Group 2) compared to patients who received only bFGF (Group 1); (b) at 6 months of follow-up, the stromal mean image brightness of the patients belonging to Group 2 was significantly lower than that of the Group 1 (p < 0.03), and, interestingly, the difference was even more evident at 12 month from the treatment (p < 0.001). Moreover, the final mean of the spherical equivalent refraction was −0.06 ± 0.2 D in Group 1 and −0.08 ± 0.3 D in Group 2, whereas the final uncorrected distance visual acuity (UDVA) was equal or superior to 20/25 in 100% of eyes in both Group 1 and 2. Post refractive patients can benefit from the administration of l-cysteine before the surgery and in association with bFGF in the early postoperative period, showing a faster corneal re-epithelization able to prevent corneal haze in the long-term recovery.

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L-Cysteine protects intestinal integrity, attenuates intestinal inflammation and oxidant stress, and modulates NF-κB and Nrf2 pathways in weaned piglets after LPS challenge

Cited by 74 publications

References 37 publications

Acute heat stress activated inflammatory signaling in porcine oxidative skeletal muscle

Acute heat stress activated inflammatory signaling in porcine oxidative skeletal muscle

Copper/zinc-loaded montmorillonite influences intestinal integrity, the expression of genes associated with inflammation, TLR4–MyD88 and TGF-β1 signaling pathways in weaned pigs after LPS challenge

Oral l-Cysteine Supplementation Enhances the Long Term-Effect of Topical Basic Fibroblast Growth Factor (bFGF) in Reducing the Corneal Haze after Photorefractive Keratectomy in Myopic Patients

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