<scp>l</scp>
            -ALANINE DEHYDROGENASE: A MECHANISM CONTROLLING THE SPECIFICITY OF AMINO ACID-INDUCED GERMINATION OF
            <i>BACILLUS CEREUS</i>
            SPORES

O’Connor, Richard J.; Halvorson, Harlyn O.

doi:10.1128/jb.82.5.706-713.1961

Cited by 54 publications

(28 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Germination. T tion (13,17,18) germination (9, 1 tinn tPmnPrqtnrP significant increase in gernination. Generally, * single-temperature optima have been reported for spore germination (13,16,17).…”

Section: Resultsmentioning

confidence: 99%

“…T tion (13,17,18) germination (9, 1 tinn tPmnPrqtnrP significant increase in gernination. Generally, * single-temperature optima have been reported for spore germination (13,16,17). The three 0 * * temperature optima (3,25, and 43 C) claimed by Thorley anomalies in the response of spores to tempera-50% RELATIVE HUMIDITY ture of germination.…”

Section: Resultsmentioning

confidence: 99%

“…It has long been recognized that the dormancy of bacterial spores is altered by the application of heat (3). Heat activation increases the rate and extent of germination and decreases the lag period before germination is apparent (7,13,17).…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Temperature on Activation, Germination, and Outgrowth of Bacillus megaterium Spores

Levinson

Hyatt

1970

J Bacteriol

View full text Add to dashboard Cite

The effects of temperature on the activation, glucose-induced germination, and outgrowth of Bacillus megaterium QM B1551 spores were investigated. There was no evidence for discontinuities in the response of spores to temperature in these processes reflecting reported thermal anomalies in the physical structure of water. Increasing the temperature of heat activation (aqueous suspensions, 5 min) increased the germinability of spores. Activation, as measured by extent of germination, was optimal after heating at 62 to 78 C, and the rate of spore germination was maximal after heat activation at 64 to 68 C. Increasing the temperature of activation above 68 C depressed the germination rate and increased the time lag before this rate was reached. Germination occurred over a wide range of temperatures, but was optimal between 28 and 38 C. The highest rate of germination was at 38 C; at lower incubation temperatures, the maximum attained rate was lower and the lag in attaining this rate was extended. Outgrowth (postgerminative development through the first cell division) of the germinated spores in Brain Heart Infusion (BHI) occurred in at least two phases-a temperature-dependent lag phase followed by a relatively temperature-independent phase of maximum outgrowth rate, during which increase in optical density was a linear function of time. Outgrowth time (time required for doubling of the initial optical density), essentially dependent on the time for completion of the lag phase, was shortest at temperatures between 34 and 40 C. The temperature-dependent lag phase was completed in a rich medium (e.g., BHI) but not in the glucose germination medium, suggesting that the endogenous reserves of the germinated spore were inadequate to support the metabolic synthetic events occurring during this period.These experiments were initiated to explore the possibility that anomalies in the physical structure of liquid water at various temperatures might result in anomalous responses in activation, germination, and outgrowth of bacterial spores. Drost-Hansen (5) has claimed that there are discontinuities or "kinks" in the physical properties of water at, or near, 15, 30, 45, and 60 C. At these specific temperatures, liquid water was said to undergo stepwise collapse in molecular structure, as estimated by such physical parameters as vibrational spectra, surface tension, compressibility, specific volume, etc., although much of this evidence for existence of discontinuities has been questioned (6). At the same specific temperatures at which thermal discontinuities in the structure of liquid water were claimed on the basis of physical measurements, anomalous thermal responses have also been reported in biological systems (4, 5).Activation conditions the dormant spore to germinate; germination results in a cell which has lost the characteristics typical of a bacterial spore (refractility, nonstainability, dipicolinic acid, heat resistance, etc.); outgrowth (postgerminative development), a period of macromolecular synthesis, results in th...

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Temperature on Activation, Germination, and Outgrowth of Bacillus megaterium Spores

Levinson

Hyatt

1970

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…Uehara & Frank (1965) reported that germination of spores of Cl. sporogenes was greater at alkaline pH values than at 7.0, and that it was markedly reduced at more acid pH values, so that at pH 6.5 and less practically no germination occurred; a situation similar to that with alanine induced germination of spores of Bacillus cereus T (O'Connor & Halvorson, 1961). No optimal pH for germination of Cl.…”

Section: (B) Heat Shockmentioning

confidence: 89%

Low Temperature Growth Characteristics of Clostridia

Roberts¹,

Hobbs²

1968

Journal of Applied Bacteriology

View full text Add to dashboard Cite

“…These data substantiated the hypothesis of an utilization of acetate during germination. This is of considerable interest in view of the fact that enzyme systems involved at the present time in the metabolism of initiators are essentially of the NAD-linked dehydrogenase type (O'Connor & Halvorson, 1961 ;Hermier & Rousseau, 1967).…”

mentioning

confidence: 99%

(Symposium on Bacterial Spores: Paper XIV). Spore Properties of Clostridia Occurring in Cheese

Bergère

Hermier

1970

Journal of Applied Bacteriology

View full text Add to dashboard Cite

l -ALANINE DEHYDROGENASE: A MECHANISM CONTROLLING THE SPECIFICITY OF AMINO ACID-INDUCED GERMINATION OF BACILLUS CEREUS SPORES

Abstract: Alanine dehydrogenase: A mechanism controlling the specificity of amino acid-induced germination of Bacillus cereus spores. J. Bacteriol. 82:706-713. 1961.-A study has been undertaken of the properties and specificity of germination of spores of Bacillus cereus strain T.

Cited by 54 publications

References 18 publications

Effects of Temperature on Activation, Germination, and Outgrowth of Bacillus megaterium Spores

Effects of Temperature on Activation, Germination, and Outgrowth of Bacillus megaterium Spores

Low Temperature Growth Characteristics of Clostridia

(Symposium on Bacterial Spores: Paper XIV). Spore Properties of Clostridia Occurring in Cheese

Contact Info

Product

Resources

About