iLight2: A near‐infrared optogenetic tool for gene transcription with low background activation
Mikhail Baloban,
Ludmila A. Kasatkina,
Vladislav V. Verkhusha
Abstract:Optogenetic tools (OTs) operating in the far‐red and near‐infrared (NIR) region offer advantages for light‐controlling biological processes in deep tissues and spectral multiplexing with fluorescent probes and OTs acting in the visible range. However, many NIR OTs suffer from background activation in darkness. Through shortening linkers, we engineered a novel NIR OT, iLight2, which exhibits a significantly reduced background activity in darkness, thereby increasing the light‐to‐dark activation contrast. The re… Show more
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