<scp><i>Morus nigra</i></scp> L. extract prolongs survival of rats with hepatocellular carcinoma

Hooshmand, Sara; Mahdinezhad, Mohammad Reza; Jamshidi, Shirin Taraz; Soukhtanloo, Mohammad; Mirzavi, Farshad; Iranshahi, Mehrdad; Hasanpour, Maede; Ghorbani, Ahmad

doi:10.1002/ptr.7056

Cited by 16 publications

(10 citation statements)

References 48 publications

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“…However, DEN signi cantly increased the expression of Akt, Wnt, and β-catenin and signi cantly decreased the expression of PTEN. These ndings are in agreement with our previous work that DEN changed the expression of PTEN, Wnt, and β-catenin while had no signi cant effect on the PI3K gene [34]. Treatment with R. turkestanicum extract (400 mg/kg) could restore the PTEN expression and downregulated the expression of Wnt4 and β-catenin genes.…”

Section: Discussionsupporting

confidence: 93%

“…Also, carcinogenesis by DEN is strongly associated with the excessive production of free radicals and decreasing the anti-oxidant enzymes which trigger time-dependent necrosis through accumulating oxidized thiol proteins [30,31]. In recent years, studies have reported bene cial effects of natural compounds in the prevention and management of cancers including HCC [32][33][34]. It has been shown the extract has anti-oxidant activity and anti-cancer effects against different cancer cell lines [11].…”

Section: Discussionmentioning

confidence: 99%

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Protective Effects of Rheum Turkestanicum Janischagainst Diethylnitrosamine-Induced Hepatocellular Carcinoma in Rats

Ghorbani¹,

Hosseini²,

Mirzavi³

et al. 2021

Preprint

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Aim of the study Hepatocellular carcinoma (HCC) is common cancer that causes many deaths worldwide. Recent studies have reported anti-cancer effects of R. turkestanicum against various cell lines including leukemia cervical tumor, and breast cancer. In this study, we aimed to identify the effect of R. turkestanicum against diethylnitrosamine (DEN)-induced HCC. Methods Wistar rats were divided into four groups of control, DEN, DEN + 100 mg/kg or 400 mg/kg of hydroethanolic extract of the plant roots. Results After four months, the animals in the DEN group showed HCC foci in the liver, an increase of hepatic lipid peroxidation, attenuation of hepatic antioxidant capacity, an increase of blood liver enzymes (ALT, AST, and ALP), bilirubin, albumin, creatinine, glucose, and reduction of the body weight. The plant extract could decrease the levels of liver enzymes, total bilirubin, direct bilirubin, albumin, urea, and creatinine in the blood. Also, the extract attenuated oxidative stress and improved pathological changes in the liver. Quantitative real-time PCR revealed a decrease in gene expression of Wnt/β-catenin and Akt and an increase in PTEN as the tumor suppressor gene. Conclusion The extract of R. turkestanicum reduced DEN-induced liver changes through inhibiting oxidative stress and attenuating the expression of Wnt/β-catenin and Akt and elevating PTEN expression.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Protective Effects of Rheum Turkestanicum Janischagainst Diethylnitrosamine-Induced Hepatocellular Carcinoma in Rats

Ghorbani¹,

Hosseini²,

Mirzavi³

et al. 2021

Preprint

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“…In DEN/PB+LMFP and DEN/PB+HMFP groups, rats were administrated exactly as DEN/PB group and additionally orally administered a low dose of MFP (LMFP) at 50 mg/kg body weight or HMFP at 100 mg/kg body weight daily for 14 successive weeks. The choice of MFP dose and treatment time was based on previous literature [ 20 , 28 , 29 ]. The detailed timelines were shown in Figure 1a .…”

Section: The Chemical Analysis Of Mfpmentioning

confidence: 99%

Mulberry fruit polysaccharides alleviate diethylnitrosamine/phenobarbital-induced hepatocarcinogenesis in vivo: the roles of cell apoptosis and inflammation

Li¹,

Sun³

et al. 2021

Bioengineered

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Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide, and chemoprevention represents a feasible treatment to reduce the mortality of this carcinoma. Mulberry fruit polysaccharides (MFP) possess immunoregulatory and anti-inflammatory effects, which have been reported to alleviate liver damage evoked by CCl4 or alcohol in previous reports. However, its chemopreventive effect against liver carcinogenesis is insufficient. The present study was aimed to investigate the possible role of MFP as a pro-apoptosis, and anti-inflammatory agent to possess its chemoprevention property. Hepatocarcinogenesis was induced by diethylnitrosamine/phenobarbital (DEN/PB) for 14 weeks. The DEN/PB-administered rats were co-treated with different doses of MFP (50 or 100 mg/kg body weight) by oral gavage for 14 weeks. Basic hepatic function indexes (AST, ALT, ALP, GGT, total bilirubin, and albumin), and hepatic tumor biomarkers (AFP, CEA, and CA19.9), together with histological assessment were performed. Besides, the hepatic apoptosis markers (Bcl-2, Bax, caspase3, and caspase9), inflammation markers (IL-1β, TNF-α, and NF-κB), and mutT homologue gene 1 (MTH1) were examined. Oral gavage of MFP inhibited the elevations of hepatic function indexes and hepatic tumor biomarkers and alleviated pathological changes in hepatic tissue. In addition, the hepatic apoptosis markers, inflammation markers, and the mRNA level of MTH1 were abnormal in DEN/PB group, which were reversed by MFP treatment. In conclusion, MFP is an effective agent that provides chemoprevention against DEN/PB-evoked hepatocarcinogenesis via inhibition of inflammation and induction of apoptosis.

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“…To date, only limited mouse and rat studies have shown a reduction in WNT4 expression in HCC models. 28,29 WNT4 is a secretory ligand, and its function has been reported in wound healing and angiogenesis. 30 The level of WNT4 varies among different cancer types.…”

Section: Diseases or Functions Annotation P-value Number Of Genementioning

confidence: 99%

New biomarkers and therapeutic targets of human liver cancer: Transcriptomic findings

Huang

et al. 2021

BioFactors

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Hepatocellular carcinoma (HCC) is one of the most common causes of cancerrelated deaths worldwide, causing 782,000 deaths in 2018. Poor prognosis and lack of treatment are the reasons for the high mortality rate of HCC. In the current study, we conducted a comparative transcriptomic analysis, followed by a series of bioinformatics analyses, including Gene Ontology (GO) enrichment analysis and Ingenuity Pathway Analysis (IPA), aiming to unfold the detailed molecular mechanisms underlying the development of HCC. In the comparative transcriptomic analysis of 10 pairs of HCC tumoral tissues and adjunct nontumoral tissues, we identified 115 common differentially expressed genes in HCC. The GO enrichment analysis of these genes highlighted alterations in the immune response, cell proliferation and DNA damage, energetic metabolism, cell-matrix adhesion, and filament assembly in HCC. In addition, the canonical pathway analysis of IPA further showed the importance of many cell-signaling pathways involved in the carcinogenesis of HCC. The findings of this study provide a cluster of novel biomarkers and molecular therapeutic targets for HCC diagnosis and treatment.

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Morus nigra L. extract prolongs survival of rats with hepatocellular carcinoma

Cited by 16 publications

References 48 publications

Protective Effects of Rheum Turkestanicum Janischagainst Diethylnitrosamine-Induced Hepatocellular Carcinoma in Rats

Protective Effects of Rheum Turkestanicum Janischagainst Diethylnitrosamine-Induced Hepatocellular Carcinoma in Rats

Mulberry fruit polysaccharides alleviate diethylnitrosamine/phenobarbital-induced hepatocarcinogenesis in vivo: the roles of cell apoptosis and inflammation

New biomarkers and therapeutic targets of human liver cancer: Transcriptomic findings

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