GmST1, which encodes a sulfotransferase, confers resistance to soybean mosaic virus strains G2 and G3

Abstract

Soybean mosaic virus (SMV) is one of the most widespread and devastating viral diseases worldwide. The genetic architecture of qualitative resistance to SMV in soybean remains unclear. Here, the Rsvg2 locus was identified as underlying soybean resistance to SMV by genome-wide association and linkage analyses. Fine mapping results showed that soybean resistance to SMV strains G2 and G3 was controlled by a single dominant gene, GmST1, on chromosome 13, encoding a sulfotransferase (SOT). A key variation at position 506 in the coding region of GmST1 associated with the structure of the encoded SOT and changed SOT activity levels between RSVG2-S and RSVG2-R alleles. In RSVG2-S allele carrier "Hefeng25", the overexpression of GmST1 carrying the RSVG2-R allele from the SMV-resistant line "Dongnong93-046" conferred resistance to SMV strains G2 and G3. Compared to Hefeng25, the accumulation of SMV was decreased in transgenic plants carrying the RSVG2-R allele. SMV infection differentiated both the accumulation of jasmonates and expression patterns of genes involved in jasmonic acid (JA) signalling, biosynthesis and catabolism in RSVG2-R and RSVG2-S allele carriers. This characterization of GmST1 suggests a new scenario explaining soybean resistance to SMV.

Understandings and future challenges in soybean functional genomics and molecular breeding

“…A genome‐wide association study (GWAS) and linkage analysis characterized Rsvg2 as encoding a sulfotransferase (ST1). Overexpression of the ST1 resistance allele enhances resistance to SMV strains G2 and G3, probably through regulating jasmonic acid signaling (Zhao et al, 2021a). Rsv3 , located on chromosome 14, was identified from the cultivar OX686 and controls the stem‐tip necrosis reaction caused by infection with strains G1–G4 (Buzzell and Tu, 1989).…”

WGCNA Reveals Hub Genes and Key Gene Regulatory Pathways of the Response of Soybean to Infection by Soybean mosaic virus

“…Based on the interaction between viruses and plants, the types of plant resistance to viruses are thought to include dominant resistance and recessive resistance [14,15]. To our knowledge, approximately 34 single dominant resistance loci for SMV located on six chromosomes have been identified and reported in soybean: chromosome 02 (Rsv4, RSC5, RSC6, RSC7, RSC8, RSC10, RSC17, RSC18A, RSC3(w), RkSC13, Rsc13, RSC9, and Rsc3k) [16][17][18][19][20][21], chromosome 03 (qTsmv-13) [22], chromosome 06 (RSC15, RSC18) [2,20], chromosome 11(RSMV-11) [23], chromosome 13 (Rsv1, Rsv5, RSC3Q, RSC11, RSC12, RSC14Q, RSC18B, RSC20, RSC-pm, RSC-ps, Rsv1-r, RSC15ZH, qSMV13, and Rsvg2) [20,[24][25][26][27][28], and chromosome 14 (Rsv3, RSC4, and rySC13) [20,29]. Among the above five resistance loci, for which gene cloning and functional analysis have been completed, Glyma.14g204700 on Rsv3 [30], Rsc4-3 on Rsc4 [31,32], GmCAL on Rsc8 [33], GmST1 on Rsvg2 [28], and a recombinant gene on Rsc3k are involved in soybean resistance to SMV [17].…”