<scp>HSP</scp>90 canonical content organizes a molecular scaffold mechanism to progress flowering

Margaritopoulou, Theoni; Kryovrysanaki, Nikoleta; Μέγκουλα, Παναγιώτα; Prassinos, Constantinos; Samakovli, Despoina; Milioni, Dimitra; Hatzopoulos, Polydefkis

doi:10.1111/tpj.13191

Cited by 39 publications

(39 citation statements)

References 56 publications

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“…After synthesis in the ribosome, the polypeptide chain rapidly folds into its characteristic and functional three-dimensional structure from a random coil. This process is accomplished through the assistance of chaperones, such as the ER-resident molecular chaperone BiP, the HSP70 family, and the HSP90 family 50–55 . The data in the present study showed that lysine residues in members of HSP70 and HSP90 were extensively crotonylated in tobacco.…”

Section: Discussionmentioning

confidence: 99%

First comprehensive proteome analysis of lysine crotonylation in seedling leaves of Nicotiana tabacum

Sun

Liu

et al. 2017

Sci Rep

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Histone crotonylation is a new lysine acylation type of post-translational modification (PTM) enriched at active gene promoters and potential enhancers in yeast and mammalian cells. However, lysine crotonylation in nonhistone proteins and plant cells has not yet been studied. In the present study, we performed a global crotonylation proteome analysis of Nicotiana tabacum (tobacco) using high-resolution LC-MS/MS coupled with highly sensitive immune-affinity purification. A total of 2044 lysine modification sites distributed on 637 proteins were identified, representing the most abundant lysine acylation proteome reported in the plant kingdom. Similar to lysine acetylation and succinylation in plants, lysine crotonylation was related to multiple metabolism pathways, such as carbon metabolism, the citrate cycle, glycolysis, and the biosynthesis of amino acids. Importantly, 72 proteins participated in multiple processes of photosynthesis, and most of the enzymes involved in chlorophyll synthesis were modified through crotonylation. Numerous crotonylated proteins were implicated in the biosynthesis, folding, and degradation of proteins through the ubiquitin-proteasome system. Several crotonylated proteins related to chromatin organization are also discussed here. These data represent the first report of a global crotonylation proteome and provide a promising starting point for further functional research of crotonylation in nonhistone proteins.

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Section: Discussionmentioning

confidence: 99%

First comprehensive proteome analysis of lysine crotonylation in seedling leaves of Nicotiana tabacum

Sun

Liu

et al. 2017

Sci Rep

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“…In Arabidopsis , HSP90, as a molecular chaperone, contributes to BR-mediated gene expression through complex formation with two major TFs, BZR1 and BES1 (Samakovli et al, 2014; Shigeta et al, 2015). A recent study reported that HSP90 is pivotal in the transition from vegetative to reproductive phase in Arabidopsis and that depletion of HSP90 mRNA levels causes extreme variation in the expression of flowering genes, and disturbs flower determination and patterning (Margaritopoulou et al, 2016). We found that a large number of HSP genes were regulated by BR treatment, indicating that HSPs also have a central function in BR-regulated petal growth in G. hybrida.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals the regulation of brassinosteroids on petal growth in Gerbera hybrida

Huang

Han

Yao

et al. 2017

PeerJ

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Gerbera hybrida is a cut-flower crop of global importance, and an understanding of the mechanisms underlying petal development is vital for the continued commercial development of this plant species. Brassinosteroids (BRs), a class of phytohormones, are known to play a major role in cell expansion, but their effect on petal growth in G. hybrida is largely unexplored. In this study, we found that the brassinolide (BL), the most active BR, promotes petal growth by lengthening cells in the middle and basal regions of petals, and that this effect on petal growth was greater than that of gibberellin (GA). The RNA-seq (high-throughput cDNA sequencing) technique was employed to investigate the regulatory mechanisms by which BRs control petal growth. A global transcriptome analysis of the response to BRs in petals was conducted and target genes regulated by BR were identified. These differentially expressed genes (DEGs) include various transcription factors (TFs) that were activated during the early stage (0.5 h) of BL treatment, as well as cell wall proteins whose expression was regulated at a late stage (10 h). BR-responsive DEGs are involved in multiple plant hormone signal pathways, hormone biosynthesis and biotic and abiotic stress responses, showing that the regulation of petal growth by BRs is a complex network of processes. Thus, our study provides new insights at the transcriptional level into the molecular mechanisms of BR regulation of petal growth in G. hybrida.

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“…In Ipomoea nil (formely Pharbitis nil, the Japanese morning glory), hsp83 was upregulated upon exposure to a photoperiod that induces flowering (Felsheim and Das, 1992). The heat shock protein Hsp90 was also reported to act as an environmental signal sensor regulating flowering time (Sangster et al, 2007) and flower development (Margaritopoulou et al, 2016). Another missense variant was found at a gene encoding for a protein kinase U-box domain-containing.…”

Section: Biological Insights Into the Genetic Basis Of Fruit-related mentioning

confidence: 99%

Insights Into the Genetic Basis of Blueberry Fruit-Related Traits Using Diploid and Polyploid Models in a GWAS Context

et al. 2018

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Polyploidization is an ancient and recurrent process in plant evolution, impacting the diversification of natural populations and plant breeding strategies. Polyploidization occurs in many important crops; however, its effects on inheritance of many agronomic traits are still poorly understood compared with diploid species. Higher levels of allelic dosage or more complex interactions between alleles could affect the phenotype expression. Hence, the present study aimed to dissect the genetic basis of fruit-related traits in autotetraploid blueberries and identify candidate genes affecting phenotypic variation. We performed a genome-wide association study (GWAS) assuming diploid and tetraploid inheritance, encompassing distinct models of gene action (additive, general, different orders of allelic interaction, and the corresponding diploidized models). A total of 1,575 southern highbush blueberry individuals from a breeding population of 117 full-sib families were genotyped using sequence capture and next-generation sequencing, and evaluated for eight fruit-related traits. For the diploid allele calling, 77,496 SNPs were detected; while 80,591 SNPs were obtained in tetraploid, with a high degree of overlap (95%) between them. A linear mixed model that accounted for population and family structure was used for the GWAS analyses. By modeling tetraploid genotypes, we detected 15 SNPs significantly associated with five fruit-related traits. Alternatively, seven significant SNPs were detected for only two traits using diploid genotypes, with two SNPs overlapping with the tetraploid scenario. Our results showed that the importance of tetraploid models varied by trait and that the use of diploid models has hindered the detection of SNP-trait associations and, consequently, the genetic architecture of some commercially important traits in autotetraploid species. Furthermore, 14 SNPs co-localized with candidate genes, five of which lead to non-synonymous amino acid changes. The potential functional significance of these SNPs is discussed.

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HSP90 canonical content organizes a molecular scaffold mechanism to progress flowering

Cited by 39 publications

References 56 publications

First comprehensive proteome analysis of lysine crotonylation in seedling leaves of Nicotiana tabacum

First comprehensive proteome analysis of lysine crotonylation in seedling leaves of Nicotiana tabacum

Transcriptome analysis reveals the regulation of brassinosteroids on petal growth in Gerbera hybrida

Insights Into the Genetic Basis of Blueberry Fruit-Related Traits Using Diploid and Polyploid Models in a GWAS Context

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