HLA‐A, HLA‐B and HLA‐DRB1 allele and haplotype diversity among volunteer bone marrow donors from Croatia

Abstract

The determination of human leucocyte antigen (HLA)-A, HLA-B and HLA-DRB1 alleles in the routine procedure of a volunteer hematopoietic stem cell (HSC) donor's registration in the Croatian Bone Marrow Donor Registry (CBMDR) is performed to enhance the odds of finding a suitable HLA compatible donor for patients in need of a HSC transplantation worldwide. However, besides its original purpose, it also provides valuable information about the HLA polymorphism among Croats. The aim of the present study was to analyse the HLA allele and haplotype frequencies in a sample of 4000 donors from CBMDR. The distribution of HLA-A, HLA-B and HLA-DRB1 alleles did not demonstrate significant differences from the data reported for other European populations. The higher frequency of B*40:02 allele in comparison with B*40:01 and DRB1*11:04 in comparison with DRB1*11:01 is interesting because it represents a difference in comparison with the Western and Northern European populations which are a main source of donors for Croatian patients. The haplotype frequencies show a greater variation and difference in comparison with data from other registries and populations; however, due to a lack of high-resolution haplotype data, comparison was possible only with a very limited number of other populations.

HLA profile of the donors in the Macedonian Bone Marrow Donor Registry

“…We identified 24 HLA‐B allele groups in Albanians and 20 in Macedonian Muslims, and HLA‐B*51, HLA‐B*18 and HLA‐B*35 had frequency >10% in both groups. HLA‐B*51 is the most common also in Albanians, Bulgarians, Croatian and Greek (Grubic et al, ; Ivanova et al, ; Papassavas et al, ; Sulcebe et al, ), whilst in Bosnians and Turks the most common are HLA‐B*44 and HLA‐B*51 (Arnaiz‐Villena, Dimitroski, et al, ; Arnaiz‐Villena, Karin, et al, ; González‐Galarza et al, ). In the countries from West Europe and Russia, the most frequent allele groups are HLA‐B*44, HLA‐B*35, HLA‐B*08 and HLA‐B*07 (González‐Galarza et al, ).…”

“…We identified 12 HLA‐DRB1 allele groups in Albanian and Macedonian Muslims (only HLA‐DRB1*09 was not detected), with HLA‐DRB1*11 (24.2%), HLA‐DRB1*13 (17.9%) and HLA‐DRB1*16 (10.9%) being the most common in Albanians, whereas in Macedonian Muslims the most common were HLA‐DRB1*11 (28.2%), HLA‐DRB1*16 (17.1%) and HLA‐DRB1*14 (17.1%). HLA‐DRB1*11 is the most common allele group in all populations on the Balkan Peninsula (Andric et al, ; A. Arnaiz‐Villena, Dimitroski, et al, ; Arnaiz‐Villena, Karin, et al, ; González‐Galarza et al, ; Grubic et al, ; Ivanova et al, ; Papassavas et al, ; Sulcebe et al, ; Vojvodić & Ademović‐Sazdanić, ). In the countries from West and North Europe, different profile of the allele groups in HLA‐DRB1 locus is present, with HLA‐DRB1*15, HLA‐DRB1*04 and HLA‐DRB1*07 being the most common (Dubois & Gebuhrer, ; González‐Galarza et al, ; Wennerström et al, ; Zahlavova et al, ), whilst in the South European population, the most frequent are HLA‐DRB1*11, HLA‐DRB1*07, HLA‐DRB1*13, HLA‐DRB1*04, HLA‐DRB1*03 and HLA‐DRB1*01 (González‐Galarza et al, ).…”

“…The most frequent were HLA‐A*01‐B*08‐C*07‐DRB1*03 (4.0%) and HLA‐A*02‐B*18‐C*07‐DRB1*11 (3.8%). HLA‐A*01‐B*08‐C*07‐DRB1*03 is the most frequent also in the majority of European countries (Andric et al, ; Grubic et al, ; Sanchez‐Velasco et al, ; Schmidt et al, ; Sulcebe et al, ), with the exception of Finland where HLA‐A*03‐B*35‐DRB1*01:01 (Wennerström et al, ) is the most frequent. HLA‐A*02‐B*18‐C*07‐DRB1*11 is the most common haplotype in Greek and Albanians from Albania and Kosovo (Papassavas et al, ; Sulcebe et al, ).…”

“…We calculated the Nei's genetic distance for HLA‐A, ‐B and ‐DRB1 for the Macedonian, Albanian and Macedonian Muslims in MBMDR and 20 European population and unrooted tree was constructed using the Neighbour‐Joining method (Saitou & Nei, ) implemented in the Phylip software. D. Middleton's website, http://www.allelefrequencies.net, was used for most of the data for the other population, accessed on October, 2017 (González‐Galarza et al, ) and also available published papers (Andric, Popadic, Jovanovic, Jaglicic, & Bojic, ; Grubic et al, ; Harbo et al, ; Inotai et al, ; Ivanova, Mihailova, Tyfekchiev, Michailova, & Naumova, ; Schmidt et al, ; Sulcebe et al, ; Zahlavova, Bendukidze, & Ivaskova, ). The populations in MBMDR were compared to these populations: Serbian (1,992), Albanian (160), Bulgarian (55), Bosnian (134), Croatian (4,000), Greece_pop7 (11,250), Turkey_pop2 (228), Slovenia_pop3 (130), Austria (200), Albanians_Kosovo (120), Italy (159,311), Hungarian (1,644), Romania (348), Germany (11,407), France Marseille (1,000), Portugal Lisbon (17,420), Russia Moscow (2,650), Sweden_pop4 (966), Norway_pop2 (576) and Czech Republic Gypsy_pop2 (46).…”

A genomic study on distribution of human leukocyte antigen ( HLA ) -A and HLA-B alleles in Lak population of Iran

“…Clinically, being acquainted with HLA distribution is a sine qua non for bone marrow donating centers [16], [17], forensic medicine [18], studies of HLA related disease such as type 2 diabetes [19], [20], [21] or multiple sclerosis [22], designing peptide vaccine and monoclonal antibodies against tumors [23], [24], infectious agents [25], [26], [27] and autoimmune disease [20], [28], as well as infertility treatment and assisted reproductive technologies [29], [30], [31].…”