<scp>GTR1</scp> and <scp>GTR2</scp> transporters differentially regulate tissue‐specific glucosinolate contents and defence responses in the oilseed crop <scp><i>Brassica juncea</i></scp>

Nambiar, Deepti M.; Kumari, Juhi; Augustine, Rehna; Kumar, Pawan; Bajpai, Prabodh K.; Bisht, Naveen C.

doi:10.1111/pce.14072

Cited by 25 publications

(28 citation statements)

References 54 publications

(98 reference statements)

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“…SULTR1;1 and SULTR1;2 are two sulfate transporters that function in Arabidopsis roots, and their orthologs in B. nigra also showed a relatively high expression in roots. GTR1 and GTR2 are responsible for transporting synthesized GSLs from leaves to seeds and roots [50,51]. Expression data also showed that BniGTR1s and BniGTR2s were predominantly expressed in roots (Figure 3C).…”

Section: Expression Patterns Of Bnigsl Genes Encoding Tfs Transporters and Proteins Involved In Co-substrate Pathwaysmentioning

confidence: 89%

Transcriptome Profiling Reveals Candidate Key Genes Involved in Sinigrin Biosynthesis in Brassica nigra

et al. 2021

Horticulturae

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Glucosinolates (GSLs) are important secondary metabolites in Brassicales related to insect and disease resistance, flavor formation, and human health. Here, we determined the GSL profile with sinigrin as the predominant GSL in Brassica nigra. A total of 184 GSL biosynthetic genes (BniGSLs) were identified in B. nigra by a genome-wide search for orthologs of 82 of the 95 known GSL genes in Arabidopsis thaliana. Transcriptome data demonstrated that at least one BniGSL was highly expressed in stems and leaves at each step of the sinigrin synthesis pathway, which ensured the synthesis of a large amount of sinigrin in B. nigra. Among these key candidates of BniGSLs, the high expression of BniMAM1-2, BniCYP79F1, and BniAOP2-1/2, and the absence of MAM3 and AOP3, may contribute remarkably to the synthesis and accumulation of sinigrin. In addition, the low expression of some key BniGSLs partially explains the low content of indolic and aromatic GSLs in B. nigra. This study provided a genetic explanation for the formation of the unique GSL profile with sinigrin as the main GSL in B. nigra. The results of this study will be valuable for further functional analysis of BniGSLs and genetic improvement of GSLs in B. nigra and other Brassica species.

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Section: Expression Patterns Of Bnigsl Genes Encoding Tfs Transporters and Proteins Involved In Co-substrate Pathwaysmentioning

confidence: 89%

Transcriptome Profiling Reveals Candidate Key Genes Involved in Sinigrin Biosynthesis in Brassica nigra

et al. 2021

Horticulturae

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“…In addition, loss of function mutation of GTR genes in B. rapa and B. juncea resulted in a 60–70% reduction in GSL accumulation in seeds ( Nour et al, 2017 ). In B. juncea , the knockdown of GTR1 had the least effects on the transport of GSL from siliques and leaves to seeds but GTR2 knockdown resulted in lower accumulation in seeds but a higher amount of GSL in siliques and leaves ( Nambiar et al, 2021 ). In Chinese kale, three GTR1 genes have been cloned as BocGTR1a , BocGTR1b , and BocGTR1c .…”

Section: Discussionmentioning

confidence: 99%

Metabolite Characteristics Analysis of Siliques and Effects of Lights on the Accumulation of Glucosinolates in Siliques of Rapeseed

Kamal

Shen

et al. 2022

Front. Plant Sci.

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Glucosinolates (GSLs) are naturally occurring secondary metabolites found in the Brassicaceae family, which mainly synthesize in the siliques with a wide range of functions. In this study, we investigated the effects of lights on metabolites in siliques of rapeseed through ultra high-performance liquid chromatography (UPLC)—heated electrospray ionization (HESI)–tandem mass spectrometry (MS/MS). A total of 249 metabolites, including 29 phenolic acids, 38 flavonoids, 22 GSLs, 93 uncalculated and 67 unknown compounds, were identified in siliques of rapeseed. Meanwhile, 62 metabolites showed significant differences after shading treatment, which were mainly GSLs and unknown compounds. Interestingly, the amounts of 10 GSLs had high accumulation levels in siliques, while the expression levels of their corresponding biosynthetic genes (AOP, GSL-OH, IGMT, and ST5a) were obviously reduced after shading treatment. Further evidence showed that the amounts of GSLs were significantly reduced in seeds, in accordance with the expression profiles of transporter genes (BnaGTRs). Our findings indicated that lights could affect the accumulation and transportation of GSLs from siliques to seeds in rapeseed. Therefore, this study facilitates a better understanding of metabolic characteristics of siliques and provides insight into the importance of light for GSLs accumulation and transportation in siliques and seeds of rapeseed.

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“…Secondly, we reason that the knock-out mutations in biosynthesis genes BnMYB28 and BnCYP79F1 described in this study confer restricted aliphatic GSL biosynthesis in the leaves. However, we speculate that a more significant phenotypic effect is realized in the seeds due to the subsequent activity of putative seed-specific GSL transporters [63; 64] .…”

Section: Discussionmentioning

confidence: 99%

Reduced glucosinolate content in oilseed rape (Brassica napus L.) by random mutagenesis of BnMYB28 and BnCYP79F1 genes

Jhingan

Harloff

Abbadi

et al. 2022

Preprint

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The presence of anti-nutritive compounds like glucosinolates (GSLs) in the rapeseed meal severely restricts its utilization as animal feed. Therefore, reducing the GSL content to <18 μmol/g dry weight in the seeds is a major breeding target. While candidate genes involved in the biosynthesis of GSLs have been described in rapeseed, comprehensive functional analyses are missing. By knocking out the aliphatic GSL biosynthesis genes BnMYB28 and BnCYP79F1 encoding an R2R3 MYB transcription factor and a cytochrome P450 enzyme, respectively, we aimed to reduce the seed GSL content in rapeseed. After expression analyses on single paralogs, we used an ethyl methanesulfonate (EMS) treated population of the inbred winter rapeseed "Express617" to detect functional mutations in the two gene families. Our results provide the first functional analysis by knock-out for the two GSL biosynthesis genes in winter rapeseed. We demonstrate that independent knock-out mutants of the two genes possessed significantly reduced seed aliphatic GSLs, primarily progoitrin. Compared to the wildtype Express617 control plants (36.3 μmol/g DW), progoitrin levels were decreased by 55.3% and 32.4% in functional mutants of BnMYB28 (16.20 μmol/g DW) and BnCYP79F1 (24.5 μmol/g DW), respectively. Our study provides a strong basis for breeding rapeseed with improved meal quality in the future.

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GTR1 and GTR2 transporters differentially regulate tissue‐specific glucosinolate contents and defence responses in the oilseed crop Brassica juncea

Cited by 25 publications

References 54 publications

Transcriptome Profiling Reveals Candidate Key Genes Involved in Sinigrin Biosynthesis in Brassica nigra

Transcriptome Profiling Reveals Candidate Key Genes Involved in Sinigrin Biosynthesis in Brassica nigra

Metabolite Characteristics Analysis of Siliques and Effects of Lights on the Accumulation of Glucosinolates in Siliques of Rapeseed

Reduced glucosinolate content in oilseed rape (Brassica napus L.) by random mutagenesis of BnMYB28 and BnCYP79F1 genes

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