<scp>GSK</scp>3b‐inhibitor lithium chloride enhances activation of Wnt canonical signaling and osteoblast differentiation on hydrophilic titanium surfaces

Galli, Carlo; Piemontese, Marilina; Lumetti, Simone; Manfredi, Edoardo; Macaluso, Guido Maria; Passeri, Giovanni

doi:10.1111/j.1600-0501.2012.02488.x

Cited by 75 publications

(68 citation statements)

References 25 publications

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“…This behavior started to manifest itself from early time-points, up to 3 h after seeding, and became more evident by 24 h of culture, a time-point by which adhesion is considered mostly stable. Even on substrates with low roughness such as Pickled surfaces, where cells exhibit differentiation behaviors quite distinctively different from SLA discs 1) , cells preferentially adhered to titanium peaks, and areas of the surface near to peaks (Fig. 6).…”

Section: Discussionmentioning

confidence: 99%

“…Micro-structured surfaces have long been used to promote the integration of endosseous implants in bone, as rough surfaces have been extensively shown to improve the differentiation of mesenchymal and bone cells, bolstering the expression of osteoblast-specific genes [1][2][3][4] . Adhesion is a key factor in determining the ability of a cell to effectively colonise a biomaterial, and thus to deposit a matrix that can serve as basis for newly formed tissue.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Osteoblasts preferentially adhere to peaks on micro-structured titanium

Lagonegro¹,

Trevisi²,

Nasi³

et al. 2018

Dent. Mater. J.

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The aim of the study was to investigate cell adhesion to micro-structured titanium. Osteoblastic MC3T3 cells were cultured on smooth (P) or sand-blasted/acid-etched (SLA) titanium discs and were observed at scanning electron microscope/focused ion beam (SEM/ FIB). Myosin II and actin microfilaments were labelled for epifluorescence microscopy. FIB revealed that cell adhesion initiated centrally and expanded to the cell periphery and that cells attached on the substrate by bridging over the titanium irregularities and adhering mostly on surface peaks. Gaps were visible between concave areas and cytoplasm and areas around ridges represented preferred attachment points for cells. A different myosin distribution was observed between samples and myosin inhibition affected cell responses. Taken together our data indicate that cells attach on micro-rough titanium by bridging over its irregularities. This is likely mediated by myosin II, whose distribution is altered in cells on SLA discs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Osteoblasts preferentially adhere to peaks on micro-structured titanium

Lagonegro¹,

Trevisi²,

Nasi³

et al. 2018

Dent. Mater. J.

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“…The use of the enhancer Wnt/beta-catenin lithium can be discussed in ALS in which the Wnt/beta-catenin pathway has been shown to be upregulated in several animal studies [32][33][34][35]. GSK-3beta-inhibitor lithium chloride enhances activation of the canonical Wnt signaling [42][43][44]. Lithium activates downstream components of the Wnt signaling pathway in vivo, leading to an increase of the beta-catenin protein.…”

Section: Als and Riluzolementioning

confidence: 99%

Amyotrophic Lateral Sclerosis: Role of the Canonical Wnt/Beta- Catenin Pathway and PPAR Gamma

Lecarpentier¹,

Vallée²

2016

Update on Amyotrophic Lateral Sclerosis

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Amyotrophic lateral sclerosis (ALS) is one of the most common adult-onset debilitating neurodegenerative diseases (NDs) which is characterized by a chronic progressive degeneration of upper and lower motor neurons, resulting in muscular atrophy, paralysis and ultimately death. It has been established that in ALS, the canonical Wnt/ beta-catenin pathway is upregulated. Peroxisome proliferator-activated receptor gamma (PPAR gamma) generally varies in opposite way compared with the Wnt/betacatenin signaling. Several studies carried out on ALS transgenic mice have shown the beneficial effects induced after treatment by PPAR agonists partly due to antiinflammatory effects induced by PPAR gamma. The coupling between the Wnt/betacatenin signaling and PPAR gamma has led to divide NDs into two classes: NDs in which the Wnt/beta-catenin pathway is upregulated whereas PPAR gamma is downregulated (ALS, Parkinson's disease, Huntington's disease and Friedreich's ataxia); and NDs in which the Wnt-beta-catenin pathway is downregulated while PPAR gamma is upregulated (Alzheimer's disease, bipolar disorder and schizophrenia).

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“…24,25 Moreover, recent studies reported that the ionic products and surface topography of bioactive materials could promote osteoblast differentiation of human MSCs via activation of canonical Wnt signaling pathway. 14,[26][27][28] But whether the canonical Wnt signaling pathway is responsible for the osteogenic and cementogenic effect of mnHAs on human periodontal ligament derived stem cells (hPDLSCs) has not been reported previously and needs to be verified.…”

Section: Introductionmentioning

confidence: 99%

Effect of micro-nano-hybrid structured hydroxyapatite bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via Wnt signaling pathway

Mao¹,

Liu²,

Zhao³

et al. 2015

IJN

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The surface structure of bioceramic scaffolds is crucial for its bioactivity and osteoinductive ability, and in recent years, human periodontal ligament stem cells have been certified to possess high osteogenic and cementogenic differential ability. In the present study, hydroxyapatite (HA) bioceramics with micro-nano-hybrid surface (mnHA [the hybrid of nanorods and microrods]) were fabricated via hydrothermal reaction of the α-tricalcium phosphate granules as precursors in aqueous solution, and the effects of mnHA on the attachment, proliferation, osteogenic and cementogenic differentiations of human periodontal ligament stem cells as well as the related mechanisms were systematically investigated. The results showed that mnHA bioceramics could promote cell adhesion, proliferation, alkaline phosphatase (ALP) activity, and expression of osteogenic/cementogenic-related markers including runt-related transcription factor 2 (Runx2), ALP, osteocalcin (OCN), cementum attachment protein (CAP), and cementum protein (CEMP) as compared to the HA bioceramics with flat and dense surface. Moreover, mnHA bioceramics stimulated gene expression of low-density lipoprotein receptor-related protein 5 ( LRP5 ) and β-catenin , which are the key genes of canonical Wnt signaling. Moreover, the stimulatory effect on ALP activity and osteogenic and cementogenic gene expression, including that of ALP, OCN, CAP, CEMP, and Runx2 of mnHA bioceramics could be repressed by canonical Wnt signaling inhibitor dickkopf1 (Dkk1). The results suggested that the HA bioceramics with mnHA could act as promising grafts for periodontal tissue regeneration.

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GSK3b‐inhibitor lithium chloride enhances activation of Wnt canonical signaling and osteoblast differentiation on hydrophilic titanium surfaces

Abstract: Stimulation of the canonical Wnt pathway promoted osteoblast differentiation on hydrophilic modSLA surfaces. Taken together, these results demonstrate that Wnt activators such as LiCl should be further tested as a possible approach to improve implant osseointegration.

Cited by 75 publications

References 25 publications

Osteoblasts preferentially adhere to peaks on micro-structured titanium

Osteoblasts preferentially adhere to peaks on micro-structured titanium

Amyotrophic Lateral Sclerosis: Role of the Canonical Wnt/Beta- Catenin Pathway and PPAR Gamma

Effect of micro-nano-hybrid structured hydroxyapatite bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via Wnt signaling pathway

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