<scp>GC</scp>/<scp>MS</scp>analysis and characterization of 2‐Hexadecen‐1‐ol and beta sitosterol from<i>Schimpera arabica</i>extract for its bioactive potential as antioxidant and antimicrobial

Hidayathulla, Syed; Shahat, Abdelaaty A.; Ahamad, Syed Rizwan; Moqbil, A.A.N. Al; Alsaid, Mansour S.; Divakar, Darshan Devang

doi:10.1111/jam.13704

Cited by 23 publications

(8 citation statements)

References 24 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As carried out earlier, the compounds found in MO2C extract were investigated using GC-MS (Agilent Technologies, USA) [ 13 ]. Those phytochemicals were identified by comparing their mass spectra to the library of the National Institute of Standards and Technology (NIST, 2004).…”

Section: Methodsmentioning

confidence: 99%

[Retracted] Evaluation of the Anticancer Potential of Morus nigra and Ocimum basilicum Mixture against Different Cancer Cell Lines: An In Vitro Evaluation

Almutairi

Alsayadi

Abutaha

et al. 2023

BioMed Research International

View full text Add to dashboard Cite

Morus nigra (M) and Ocimum basilicum (O) mixture (MO2) extract was extracted using hexane (MO2H), chloroform (MO2C), ethyl acetate (MO2E), and methanol (MO2M) in a Soxhlet apparatus. The cytotoxicity was evaluated using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. The IC50 values of the MO2C-treated cancer cells were 11.31 μg/mL (MDA-MB-231), 15.45 μg/mL (MCF-7), 18.9 μg/mL (HepG2), 26.33 μg/mL (Huh-7), 30.17 μg/mL (LoVo), and 36.76 μg/mL (HCT116). MO2C-treated cells showed cellular and nuclear morphological alterations like chromatin condensation and formation of apoptotic bodies as observed using light and fluorescent microscopy. The antioxidant and anti-inflammatory properties were investigated in vitro using 2,2 ′ -diphenyl-1-picrylhydrazyl (DPPH) and egg albumin denaturation assays. It was evident that the MO2M extract exhibited the highest antioxidant activity (18.13%), followed by the MO2E extract (12.25%), MO2C extract (9.380%), and MO2H extract (6.31%). The highest inhibition percentage of albumin denaturation was observed in MO2H (28.54%), followed by MO2M (4.32%) at 0.2 and 0.1 mg/mL concentrations, respectively. The compounds identified using gas chromatography-mass spectrometry (GC-MS) analysis for MO2C extract were α-trans-bergamotene, germacrene D, selin-4,7(11)-diene, 2 tridecen-1-ol, and 2-decen-1-ol. The present study reveals that MO2C has promising anticancer activity and may serve as a potent polyherbal extract in cancer treatment.

show abstract

Section: Methodsmentioning

confidence: 99%

[Retracted] Evaluation of the Anticancer Potential of Morus nigra and Ocimum basilicum Mixture against Different Cancer Cell Lines: An In Vitro Evaluation

Almutairi

Alsayadi

Abutaha

et al. 2023

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Seeds were collected from Pithecellobium dulce tree (Hail region, Saudi Arabia) and washed with sterile distilled water. Around 400 grams of P. dulce seed was ground to a coarse powder using an electrical grinding mill and extracted by cold maceration in 80% methanol (Sigma-Aldrich, MO, USA) at room temperature [ 19 ]. It was then left standing for 3-4 days with an occasional shaking to produce the crude extract.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Potential of Pithecellobium dulce Seed Extract against Pathogenic Bacteria: In Silico and In Vitro Evaluation

Aldarhami

Bazaid

Alhamed

et al. 2023

BioMed Research International

View full text Add to dashboard Cite

Clinical multi-drug-resistant bacteria continue to be a serious health problem. Plant-derived molecules are an important source of bioactive compounds to counteract these pathogenic bacteria. In this paper, we studied the chemical composition of the methanol (80%) extract from Pithecellobium dulce seed (Hail, Saudi Arabia) and its ability to inhibit the growth of clinically relevant multi-drug-resistant bacteria. Molecular docking analysis was performed to predict the best compounds with low binding energy and high affinity to interact with two Staphylococcus aureus receptors. Data showed that P. dulce extract is a rich source of D-turanose (55.82%), hexadecanoic acid (11.56%), indole-1-acetic acid (11.42%), inositol (5.78%), and octadecanoic acid (4.36%). The obtained extract showed antibacterial activity towards tested clinical bacterial strains with MIC values ranging from 233 mg/mL for Acinetobacter baumannii to 300 mg/mL for S. aureus and Escherichia coli. Turanose interaction has resulted in -7.4 and -6.6 kcal/mol for 1JIJ and 2XCT macromolecules, while inositol showed energy values (−7.2 and −5.4 kcal/mol) for the same receptors. Multiple identified compounds showed desirable bioavailability properties indicating its great potential therapeutic use in human. Overall, current investigation highlights the possible use of P. dulce extract as a valuable source for drug development against pathogenic drug-resistant bacteria.

show abstract

“…The gas chromatography-mass spectroscopy (GC-MS) analysis was performed in a Perkin Elmer Clarus 600 gas chromatograph inked to a mass spectrometer essentially same as described previously [50].…”

Section: Methodsmentioning

confidence: 99%

Bitter gourd (Momordica charantia) possess developmental toxicity as revealed by screening the seeds and fruit extracts in zebrafish embryos

Farooq

Abutaha

Nasr

et al. 2019

BMC Complement Altern Med

View full text Add to dashboard Cite

Background Bitter gourd ( Momordica charantia ) has attracted the focus of researchers owing to its excellent anti-diabetic action. The beneficial effect of Momordica charantia on heart has been reported by in vitro and in vivo studies. However the developmental toxicity or potential risk of M. charantia on fetus heart development is largely unknown. Hence this study was designed to find out the developmental toxicity of M. charantia using zebrafish ( Danio rerio ) embryos. Methods The crude extracts were prepared from fruit and seeds of M. charantia. The Zebrafish embryos were exposed to serial dilution of each of the crude extract. The biologically active fractions were fractionated by C18 column using high pressure liquid chromatography. Fourier-transform infrared spectroscopy and gas chromatography coupled with mass spectrophotometry was done to identify chemical constituents in fruit and seed extract of M. charantia . Results The seed extract of M. charantia was lethal with LD 50 values of 50 μg/ml to zebrafish embryos and multiple anomalies were observed in zebrafish embryos at sub-lethal concentration. However, the fruit extract was much safe and exposing the zebrafish embryos even to 200 μg/ml did not result any lethality. The fruit extract induced severe cardiac hypertrophy in treated embryos. The time window treatment showed that M. charantia perturbed the cardiac myoblast specification process in treated zebrafish embryos. The Fourier-transform infrared spectroscopy analyses revealed diverse chemical group in the active fruit fraction and five new type of compounds were identified in the crude seeds extract of M. charantia by gas chromatography and mass spectrophotometry. Conclusion The teratogenicity of seeds extract and cardiac toxicity by the fruit extract of M. charantia warned that the supplementation made from the fruit and seeds of M. charantia should be used with much care in pregnant diabetic patients to avoid possible damage to developing fetus. Electronic supplementary material The online version of this article (10.1186/s12906-019-2599-0) contains supplementary material, which is available to authorized users.

show abstract

GC/MSanalysis and characterization of 2‐Hexadecen‐1‐ol and beta sitosterol fromSchimpera arabicaextract for its bioactive potential as antioxidant and antimicrobial

Cited by 23 publications

References 24 publications

[Retracted] Evaluation of the Anticancer Potential of Morus nigra and Ocimum basilicum Mixture against Different Cancer Cell Lines: An In Vitro Evaluation

[Retracted] Evaluation of the Anticancer Potential of Morus nigra and Ocimum basilicum Mixture against Different Cancer Cell Lines: An In Vitro Evaluation

Antimicrobial Potential of Pithecellobium dulce Seed Extract against Pathogenic Bacteria: In Silico and In Vitro Evaluation

Bitter gourd (Momordica charantia) possess developmental toxicity as revealed by screening the seeds and fruit extracts in zebrafish embryos

Contact Info

Product

Resources

About