<scp>FIT</scp> and <scp>bHLH</scp> Ib transcription factors modulate iron and copper crosstalk in Arabidopsis

Cai, Yuerong; Li, Yang; Liang, Gang

doi:10.1111/pce.14000

Cited by 52 publications

(43 citation statements)

References 52 publications

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“…In Arabidopsis seedlings, AtbHLH105/IAA-LEUCINE RESISTANT 3 ( ILR3 ) is involved in the regulation of iron balance [ 74 ], and other bHLH genes, such as AtbHLH38, AtbHLH39 , AtbHLH100, AtbHLH101 , and AtbHLH115 , have been confirmed to play important roles in iron uptake under iron-deficient conditions [ 75 , 76 ]. Other roles of bHLH genes ( AtbHLH38 and AtbHLH39 ) were also identified in Arabidopsis to respond to copper deficiency [ 77 ]. In rice, the iron-related transcription factor 3 ( OsIRO3 ) that belongs to the bHLH gene family, plays a critical role in maintaining iron homeostasis in an iron-deficient environment [ 78 , 79 ].…”

Section: Roles Of Bhlh Tfs In Plant Stress Tolerancementioning

confidence: 99%

Current Understanding of bHLH Transcription Factors in Plant Abiotic Stress Tolerance

Guo

Sun

et al. 2021

IJMS

123

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Named for the characteristic basic helix-loop-helix (bHLH) region in their protein structure, bHLH proteins are a widespread transcription factor class in eukaryotes. bHLHs transcriptionally regulate their target genes by binding to specific positions on their promoters and thereby direct a variety of plant developmental and metabolic processes, such as photomorphogenesis, flowering induction, shade avoidance, and secondary metabolite biosynthesis, which are important for promoting plant tolerance or adaptation to adverse environments. In this review, we discuss the vital roles of bHLHs in plant responses to abiotic stresses, such as drought, salinity, cold, and iron deficiency. We suggest directions for future studies into the roles of bHLH genes in plant and discuss their potential applications in crop breeding.

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Section: Roles Of Bhlh Tfs In Plant Stress Tolerancementioning

confidence: 99%

Current Understanding of bHLH Transcription Factors in Plant Abiotic Stress Tolerance

Guo

Sun

et al. 2021

IJMS

123

View full text Add to dashboard Cite

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“…However, under Fe sufficiency, as a key cis-acting element of plant iron homeostasis, IDRS (irondependent regulatory sequence, which is bound by unknown factor X) is responsible for the upregulation of ferritin genes FER2, FER3, and FER4, whereas the central regulator FIT is inactivated to inhibit the expression of FRO2 and IRT1 (Bournier et al, 2013;Chen et al, 2018). By contrast, Fe deficiency activates the interacting bHLH IVc (bHLH34/104/105/115) and bHLH121 complex to positively regulate the expression of bHLH Ib (bHLH38/39/100/101) and FIT (Cai et al, 2021). The bHLH Ib and FIT interact with each other to induce the expression of Fe uptake-related genes FRO2 and IRT1 as well as PHO1 Pi transporter genes PHO1; H1 and PHO1;1 (Yi and Guerinot, 1996;Vert et al, 2002;Chen et al, 2018) for Pi homeostasis (Figure 4B).…”

Section: Molecular Basis Of Pi and Fe Interactions In Plantsmentioning

confidence: 99%

Cross-Talks Between Macro- and Micronutrient Uptake and Signaling in Plants

et al. 2021

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In nature, land plants as sessile organisms are faced with multiple nutrient stresses that often occur simultaneously in soil. Nitrogen (N), phosphorus (P), sulfur (S), zinc (Zn), and iron (Fe) are five of the essential nutrients that affect plant growth and health. Although these minerals are relatively inaccessible to plants due to their low solubility and relative immobilization, plants have adopted coping mechanisms for survival under multiple nutrient stress conditions. The double interactions between N, Pi, S, Zn, and Fe have long been recognized in plants at the physiological level. However, the molecular mechanisms and signaling pathways underlying these cross-talks in plants remain poorly understood. This review preliminarily examined recent progress and current knowledge of the biochemical and physiological interactions between macro- and micro-mineral nutrients in plants and aimed to focus on the cross-talks between N, Pi, S, Zn, and Fe uptake and homeostasis in plants. More importantly, we further reviewed current studies on the molecular mechanisms underlying the cross-talks between N, Pi, S, Zn, and Fe homeostasis to better understand how these nutrient interactions affect the mineral uptake and signaling in plants. This review serves as a basis for further studies on multiple nutrient stress signaling in plants. Overall, the development of an integrative study of multiple nutrient signaling cross-talks in plants will be of important biological significance and crucial to sustainable agriculture.

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“…In addition, MYB transcription factor-binding motifs were identified in promoter sequences of laccase 20 and laccase 13. In Arabidopsis , copper has been shown to induce expression changes of transcription factors, including bHLH, MYB, and ERF (Jakubowicz et al, 2010 ; Perea-García et al, 2010 ; Cai et al, 2021 ). Combined, these results suggest that transcription factors, such as ERF, MYB, and bHLH, play a role in copper-induced SalA biosynthesis in S. miltiorrhiza .…”

Section: Discussionmentioning

confidence: 99%

Integrative Omics Analyses Reveal the Effects of Copper Ions on Salvianolic Acid Biosynthesis

et al. 2021

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Salvianolic acids, a group of secondary metabolites produced by Salvia miltiorrhiza, are widely used for treating cerebrovascular diseases. Copper is recognized as a necessary microelement and plays an essential role in plant growth. At present, the effect of copper on the biosynthesis of SalAs is unknown. Here, an integrated metabolomic and transcriptomic approach, coupled with biochemical analyses, was employed to dissect the mechanisms by which copper ions induced the biosynthesis of SalAs. In this study, we identified that a low concentration (5 μM) of copper ions could promote growth of S. miltiorrhiza and the biosynthesis of SalAs. Results of the metabolomics analysis showed that 160 metabolites (90 increased and 70 decreased) were significantly changed in S. miltiorrhiza treated with low concentration of copper ions. The differential metabolites were mainly involved in amino acid metabolism, the pentose phosphate pathway, and carbon fixation in photosynthetic organisms. The contents of chlorophyll a, chlorophyll b, and total chlorophyll were significantly increased in leaves of low concentration of copper-treated S. miltiorrhiza plants. Importantly, core SalA biosynthetic genes (laccases and rosmarinic acid synthase), SalA biosynthesis-related transcription factors (MYBs and zinc finger CCCH domain-containing protein 33), and chloroplast proteins-encoding genes (blue copper protein and chlorophyll-binding protein) were upregulated in the treated samples as indicated by a comprehensive transcriptomic analysis. Bioinformatics and enzyme activity analyses showed that laccase 20 contained copper-binding motifs, and its activity in low concentration of copper ions-treated S. miltiorrhiza was much higher than that in the control. Our results demonstrate that enhancement of copper ions of the accumulation of SalAs might be through regulating laccase 20, MYBs, and zinc finger transcription factors, and photosynthetic genes.

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FIT and bHLH Ib transcription factors modulate iron and copper crosstalk in Arabidopsis

Cited by 52 publications

References 52 publications

Current Understanding of bHLH Transcription Factors in Plant Abiotic Stress Tolerance

Current Understanding of bHLH Transcription Factors in Plant Abiotic Stress Tolerance

Cross-Talks Between Macro- and Micronutrient Uptake and Signaling in Plants

Integrative Omics Analyses Reveal the Effects of Copper Ions on Salvianolic Acid Biosynthesis

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