<scp>DNA</scp>Replication in Humans

DePamphilis, Melvin L.

doi:10.1002/0471203076.emm0400

Cited by 6 publications

(9 citation statements)

References 40 publications

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“…In bacteria and viruses, replication initiates from a single genomic site in every cell, called the origin of replication (1). Eukaryotic DNA replication is characterized by a higher degree of uncertainty; hundreds to thousands of potential origins exist along the genome that fire with varying efficiencies and at different times during S-phase (2)(3)(4)(5). Although the spatial and temporal pattern of DNA replication is not strictly defined in eukaryotic cells, each cell in a population must complete the replication process in an accurate and timely manner.…”

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confidence: 99%

“…Similarly, DNA replication can initiate from any DNA fragment introduced on a plasmid in cultured mammalian cells and at random sites within each fragment (11). In the genomic context, specific regions have origin activity (12,13), but these lack clear sequence characteristics and fire in only a fraction of the cells (2). As a result, initiation events differ between cells in a population.…”

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confidence: 99%

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Stochastic hybrid modeling of DNA replication across a complete genome

Lygeros

Koutroumpas

Dimopoulos

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

100

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confidence: 99%

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confidence: 99%

Stochastic hybrid modeling of DNA replication across a complete genome

Lygeros

Koutroumpas

Dimopoulos

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

100

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“…All cells must accurately duplicate and segregate their chromosomal DNA, and failure to do so is a critical underlying cause of >80 different human diseases and genetic disorders (1). Replisomes are multiprotein assemblies that are responsible for DNA replication in all cells (2)(3)(4).…”

Section: Introductionmentioning

confidence: 99%

Single-Molecule DNA Polymerase Dynamics at a Bacterial Replisome in Live Cells

Liao

Schroeder

et al. 2016

Biophysical Journal

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PolC is one of two essential replicative DNA polymerases found in the Gram-positive bacterium Bacillus subtilis. The B. subtilis replisome is eukaryotic-like in that it relies on a two DNA polymerase system for chromosomal replication. To quantitatively image how the replicative DNA polymerase PolC functions in B. subtilis, we applied photobleaching-assisted microscopy, three-dimensional superresolution imaging, and single-particle tracking to examine the in vivo behavior of PolC at single-molecule resolution. We report the stoichiometry of PolC proteins within each cell and within each replisome, we elucidate the diffusion characteristics of individual PolC molecules, and we quantify the exchange dynamics for PolC engaged in lagging strand synthesis. We show that PolC is highly dynamic: this DNA polymerase is constantly recruited to and released from a centrally located replisome, providing, to our knowledge, new insight into the organization and dynamics of the replisome in bacterial cells.

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“…Eukaryotic cells have multiple replication origins that fire with various efficiencies and timings. To initiate DNA replication, many proteins assemble on, and dissociate from, origins (See for reviews DePamphilis, 2006;Masai et al, 2010). First, the sixsubunit origin recognition complex (Orc, Orc1-6) associates with replication origins throughout the cell cycle in budding yeast, and the hexameric Mcm2-7 complex, which is a catalytic core of DNA helicase, is loaded onto the Orc-bound origins with the aid of Cdc6 and Cdt1 to form the pre-replicative complex (pre-RC) from late M to G1 phases when cyclin-dependent kinase (CDK) is not activated (Fig.…”

Section: Introductionmentioning

confidence: 99%

Initiation of chromosomal DNA replication in eukaryotic cells; contribution of yeast genetics to the elucidation

Araki

2011

Genes Genet. Syst.

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Chromosomal DNA replication is a fundamental process in the transmission of genetic information through generations. While the molecular mechanism of DNA replication has been studied for a long time, knowledge regarding this process in eukaryotic cells has advanced rapidly in the past 20 years. Yeast genetics contributed profoundly to this rapid advancement. Reverse genetics and genetic screenings identified all genes encoding replication proteins in budding yeast. Moreover, the genetic interactions that were used in screenings and analyses provided an insight into the molecular mechanism of chromosomal DNA replication. Further studies showed that complicated but sophisticated mechanisms govern chromosomal DNA replication. The retrospective view of the genetic approaches used to elucidate DNA replication in eukaryotes, together with current knowledge, tell us the reasons why some of the genetic screenings are successful, and also provide ideas for future directions.

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DNAReplication in Humans

Cited by 6 publications

References 40 publications

Stochastic hybrid modeling of DNA replication across a complete genome

Stochastic hybrid modeling of DNA replication across a complete genome

Single-Molecule DNA Polymerase Dynamics at a Bacterial Replisome in Live Cells

Initiation of chromosomal DNA replication in eukaryotic cells; contribution of yeast genetics to the elucidation

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