<scp>DNA</scp>Polymerases

Wang, Teresa S.‐F.

doi:10.1002/0471203076.emm0246

Cited by 2 publications

(2 citation statements)

References 152 publications

(190 reference statements)

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“…The expression of human pol-prim genes is upregulated at the level of transcription when cells leave a quiescent state to enter the mitotic cell cycle, but the amount of pol-prim transcript does not fluctuate once mammalian cells are actively cycling [34].…”

Section: Regulation Of Pol-prim During the Cell Cyclementioning

confidence: 99%

The DNA Polymerase _-Primase Complex: Multiple Functions and Interactions

Muzi-Falconi

Giannattasio

Foiani

et al. 2003

The Scientific World JOURNAL

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DNA polymerase _ (pol _) holds a special position among the growing family of eukaryotic DNA polymerases. In fact, pol _ is associated with DNA primase to form a four subunit complex and, as a consequence, is the only enzyme able to start DNA synthesis de novo. Because of this peculiarity the major role of the DNA polymerase _-primase complex (pol-prim) is in the initiation of DNA replication at chromosomal origins and in the discontinuous synthesis of Okazaki fragments on the lagging strand of the replication fork. However, pol-prim seems to play additional roles in other complex cellular processes, such as the response to DNA damage, telomere maintenance, and the epigenetic control of higher order chromatin assembly.

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Section: Regulation Of Pol-prim During the Cell Cyclementioning

confidence: 99%

The DNA Polymerase _-Primase Complex: Multiple Functions and Interactions

Muzi-Falconi

Giannattasio

Foiani

et al. 2003

The Scientific World JOURNAL

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“…In eukaryotic cells, an evolutionarily conserved four-subunit enzyme complex, DNA polymerase ␣-primase, is the principal enzyme that initiates DNA synthesis (Wang, 1996). The primase in this enzyme complex synthesizes an RNA primer.…”

Section: Introductionmentioning

confidence: 99%

Role of Fission Yeast Primase Catalytic Subunit in the Replication Checkpoint

Griffiths

Liu

Nurse

et al. 2001

MBoC

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To investigate the cell cycle checkpoint response to aberrant S phase-initiation, we analyzed mutations of the two DNA primase subunit genes of Schizosaccharomyces pombe, spp1 ϩ and spp2 ϩ (S. pombe primase 1 and 2). spp1 ϩ encodes the catalytic subunit that synthesizes the RNA primer, which is then utilized by Pol␣ to synthesize the initiation DNA. Here, we reported the isolation of the fission yeast spp1 ϩ gene and cDNA and the characterization of Spp1 protein and its cellular localization during the cell cycle. Spp1 is essential for cell viability, and thermosensitive mutants of spp1 ϩ exhibit an allele-specific abnormal mitotic phenotype. Mutations of spp1 ϩ reduce the steady-state cellular levels of Spp1 protein and compromised the formation of Pol␣-primase complex. The spp1 mutant displaying an aberrant mitotic phenotype also fails to properly activate the Chk1 checkpoint kinase, but not the Cds1 checkpoint kinase. Mutational analysis of Pol␣ has previously shown that activation of the replication checkpoint requires the initiation of DNA synthesis by Pol␣. Together, these have led us to propose that suboptimal cellular levels of pol␣-primase complex due to the allele-specific mutations of Spp1 might not allow Pol␣ to synthesize initiation DNA efficiently, resulting in failure to activate a checkpoint response. Thus, a functional Spp1 is required for the Chk1-mediated, but not the Cds1-mediated, checkpoint response after an aberrant initiation of DNA synthesis.

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DNAPolymerases

Cited by 2 publications

References 152 publications

The DNA Polymerase _-Primase Complex: Multiple Functions and Interactions

The DNA Polymerase _-Primase Complex: Multiple Functions and Interactions

Role of Fission Yeast Primase Catalytic Subunit in the Replication Checkpoint

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