<scp>DNA</scp> methylation pattern of apoptosis‐related genes in ameloblastoma

Costa, S.R.C.F.; Pereira, Núbia Braga; Pereira, Karuza Maria Alves; Campos, Kelma; Castro, WH de; Diniz, MG; Gomes, Ciro Martins; Gomez, Ricardo Santiago

doi:10.1111/odi.12661

Cited by 15 publications

(10 citation statements)

References 44 publications

(55 reference statements)

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“…Moreover, miR-10b overexpression and HOXA1 knockdown resulted in aberrant DNMT expression and an increased embryo apoptosis ratio. Previous findings in human cancer cells have shown that cell apoptosis and cell proliferation are related to DNA methylation, and DNA methylation can help inactivate apoptotic pathways at several points (Cho et al, 2011; Ye et al, 2016; Costa et al, 2017; Loginov et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Bta-miR-10b Secreted by Bovine Embryos Negatively Impacts Preimplantation Embryo Quality

et al. 2019

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In a previous study, we found miR-10b to be more abundant in a conditioned culture medium of degenerate embryos compared to that of blastocysts. Here, we show that miR-10b mimics added to the culture medium can be taken up by embryos. This uptake results in an increase in embryonic cell apoptosis and aberrant expression of DNA methyltransferases ( DNMTs ). Using several algorithms, Homeobox A1 ( HOXA1 ) was identified as one of the potential miR-10b target genes and dual-luciferase assay confirmed HOXA1 as a direct target of miR-10b. Microinjection of si- HOXA1 into embryos also resulted in an increase in embryonic cell apoptosis and downregulation of DNMTs . Cell progression analysis using Madin–Darby bovine kidney cells (MDBKs) showed that miR-10b overexpression and HOXA1 knockdown results in suppressed cell cycle progression and decreased cell viability. Overall, this work demonstrates that miR-10b negatively influences embryo quality and might do this through targeting HOXA1 and/or influencing DNA methylation.

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Section: Discussionmentioning

confidence: 99%

Bta-miR-10b Secreted by Bovine Embryos Negatively Impacts Preimplantation Embryo Quality

et al. 2019

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“…The samples were incubated at 37°C for 6 h. Then quantitative PCR (qPCR) was performed using the RT2 SYBR Green ROX qPCR Master Mix (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations in a Step One Plus Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). Threshold cycle (Ct) values were utilized to calculate the percentages of methylated (M) and unmethylated (UM) DNA, using a quantization algorithm provided by the manufacturer to normalize the amount of DNA in each digestion against the total amount of input DNA in the mock digestion, using the Excel macro-spreadsheet supplied by the manufacturer (Qiagen, Hilden, Germany) [25-27].…”

Section: Methodsmentioning

confidence: 99%

BjussuLAAO-II induces cytotoxicity and alters DNA methylation of cell-cycle genes in monocultured/co-cultured HepG2 cells

Machado

Aissa

Ribeiro

et al. 2019

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: The use of animal venoms and their toxins as material sources for biotechnological applications has received much attention from the pharmaceutical industry. L-amino acid oxidases from snake venoms (SV-LAAOs) have demonstrated innumerous biological effects and pharmacological potential against different cancer types. Hepatocellular carcinoma has increased worldwide, and the aberrant DNA methylation of liver cells is a common mechanism to promote hepatic tumorigenesis. Moreover, tumor microenvironment plays a major role in neoplastic transformation. To elucidate the molecular mechanisms responsible for the cytotoxic effects of SV-LAAO in human cancer cells, this study aimed to evaluate the cytotoxicity and the alterations in DNA methylation profiler in the promoter regions of cell-cycle genes induced by BjussuLAAO-II, an LAAO from Bothrops jaracussu venom, in human hepatocellular carcinoma (HepG2) cells in monoculture and co-culture with endothelial (HUVEC) cells. Methods: BjussuLAAO-II concentrations were 0.25, 0.50, 1.00 and 5.00 μg/mL. Cell viability was assessed by MTT assay and DNA methylation of the promoter regions of 22 cell-cycle genes by EpiTect Methyl II PCR array. Results: BjussuLAAO-II decreased the cell viability of HepG2 cells in monoculture at all concentrations tested. In co-culture, 1.00 and 5.00 μg/mL induced cytotoxicity ( p < 0.05). BjussuLAAO-II increased the methylation of CCND1 and decreased the methylation of CDKN1A in monoculture and GADD45A in both cell-culture models ( p < 0.05). Conclusion: Data showed BjussuLAAO-II induced cytotoxicity and altered DNA methylation of the promoter regions of cell-cycle genes in HepG2 cells in monoculture and co-culture models. We suggested the analysis of DNA methylation profile of GADD45A as a potential biomarker of the cell cycle effects of BjussuLAAO-II in cancer cells. The tumor microenvironment should be considered to comprise part of biotechnological strategies during the development of snake-toxin-based novel drugs.

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“…Another possible mechanism contributing to tumorigenesis of ameloblastoma seems to be DNA hypomethylation of transcription related genes. A recent study showed a lower methylation rate significantly of the promoter region of BCL‐2 in ameloblastomas in comparison with regular dental follicles (Costa et al., ).…”

Section: Recent Molecular Pathogenic Findings and Implications For Tamentioning

confidence: 99%

Ameloblastoma—Clinical, radiological, and therapeutic findings

Kreppel

Zöller

2018

Oral Diseases

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Ameloblastoma are the most common odontogenic tumor. As they usually do not form metastasis, they are considered as benign tumors with a locally invasive growth pattern and destruction of the jaws and the surrounding tissue (Oral Diseases, 23, 2017, 199). This article focuses on clinical, radiological, and therapeutic findings, which may influence diagnosis and treatment of ameloblastoma in the future.

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DNA methylation pattern of apoptosis‐related genes in ameloblastoma

Abstract: On the basis of our results, the transcription of the apoptosis-related gene BCL2L11 is possibly regulated by promoter DNA methylation in ameloblastoma. The biological significance of this finding in ameloblastoma pathobiology remains to be clarified.

Cited by 15 publications

References 44 publications

Bta-miR-10b Secreted by Bovine Embryos Negatively Impacts Preimplantation Embryo Quality

Bta-miR-10b Secreted by Bovine Embryos Negatively Impacts Preimplantation Embryo Quality

BjussuLAAO-II induces cytotoxicity and alters DNA methylation of cell-cycle genes in monocultured/co-cultured HepG2 cells

Ameloblastoma—Clinical, radiological, and therapeutic findings

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