<scp>DNA</scp> damage and cell apoptosis induced by fungicide difenoconazole in mouse mononuclear macrophage <scp>RAW264</scp>.7

Li, Ruirui; Liu, Bin; Xu, Wenping; Yu, Lvnan; Zhang, Cheng; Cheng, Jiagao; Tao, Liming; Li, Zhong; Zhang, Yang

doi:10.1002/tox.23432

Cited by 11 publications

(3 citation statements)

References 51 publications

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“…Genotoxicity, which has a damaging effect on DNA integrity, triggers apoptosis in macrophages [ 36 , 37 ]. In addition, TEGDMA induces DNA damage in gingival fibroblasts, lymphocytes, and oral keratinocytes [ 38 , 39 , 40 ]. The results obtained from the MN and comet assays highlight that TEGDMA induces MN formation and DNA damage parameters, including tail length and tail moment [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Rutin on Triethylene Glycol Dimethacrylate-Induced Toxicity through the Inhibition of Caspase Activation and Reactive Oxygen Species Generation in Macrophages

Yang

Chang

Yeh

et al. 2022

IJMS

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Rutin, also called quercetin-3-rhamnosyl glucoside, is a natural flavonol glycoside present in many plants. Rutin is used to treat various diseases, such as inflammation, diabetes, and cancer. For polymeric biomaterials, triethylene glycol dimethacrylate (TEGDMA) is the most commonly used monomer and serves as a restorative resin, a dentin bonding agent and sealant, and a bone cement component. Overall, TEGDMA induces various toxic effects in macrophages, including cytotoxicity, apoptosis, and genotoxicity. The aim of this study was to investigate the protective mechanism of rutin in alleviating TEGDMA-induced toxicity in RAW264.7 macrophages. After treatment with rutin, we assessed the cell viability and apoptosis of TEGDMA-induced RAW264.7 macrophages using an methylthiazol tetrazolium (MTT) assay and Annexin V-FITC/propidium iodide assay, respectively. Subsequently, we assessed the level of genotoxicity using comet and micronucleus assays, assessed the cysteinyla aspartate specific proteinases (caspases) and antioxidant enzyme (AOE) activity using commercial kits, and evaluated the generation of reactive oxygen species (ROS) using a dichlorodihydrofluorescein diacetate (DCFH-DA) assay. We evaluated the expression of heme oxygenase (HO)-1, the expression of nuclear factor erythroid 2 related factor (Nrf-2), and phosphorylation of AMP activated protein kinase (AMPK) using the Western blot assay. The results indicated that rutin substantially reduced the level of cytotoxicity, apoptosis, and genotoxicity of TEGDMA-induced RAW264.7 macrophages. Rutin also blocked the activity of caspase-3, caspase-8, and caspase-9 in TEGDMA-stimulated RAW264.7 macrophages. In addition, it decreased TEGDMA-induced ROS generation and AOE deactivation in macrophages. Finally, we found that TEGDMA-inhibited slightly the HO-1 expression, Nrf-2 expression, and AMPK phosphorylation would be revered by rutin. In addition, the HO-1 expression, Nrf-2 expression, and AMPK phosphorylation was enhanced by rutin. These findings indicate that rutin suppresses TEGDMA-induced caspase-mediated toxic effects through ROS generation and antioxidative system deactivation through the Nrf-2/AMPK pathway. Therefore, rutin has the potential to serve as a novel antitoxicity agent for TEGDMA in RAW264.7 macrophages.

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Section: Discussionmentioning

confidence: 99%

Protective Effect of Rutin on Triethylene Glycol Dimethacrylate-Induced Toxicity through the Inhibition of Caspase Activation and Reactive Oxygen Species Generation in Macrophages

Yang

Chang

Yeh

et al. 2022

IJMS

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“…However, these fertilizers cause damage to the environment, human and animal health, and resistance to phytopathogens (Sabiha-Javied et al, 2023). In addition, they cause soil erosion, salinity and accumulation of heavy metals, air pollution and increases in the greenhouse effect (Li et al, 2022;Wang et al, 2022). Therefore, it is necessary to search for alternatives to decrease fertilizers use and that do not cause damage to the environment while increasing yield (Candido et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Relations Between the Mass of the Fruits, the Number of Fruits Per Plant and the Agricultural Yield in Pepper, With Increasing Silicon Doses

Reyes-Pérez,

Rivas-García,

Rodríguez-Rodríguez

et al. 2024

Trop Subtrop Agroecosyst

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Background: Statistical relations between the components of agricultural yield and the yield itself after Si treatment as possible non-destructive methods are important for predicting and monitoring the harvest. Objective: To measure the relationships between the mass of the fruit, the number of fruits per plant and the agricultural yield in pepper, with increasing silicon doses. Methodology: The research was carried out at Quevedo State Technical University, Ecuador in a completely randomized design and three repetitions per treatment. The treatments used were 0, 20, 25 and 30 g potassium silicate plant-1. The variables evaluated were fruit weight, number of fruits per plant and agricultural yield at the time of harvest. Path analysis, multiple and single linear regressions, and nonlinear regressions were performed. Results: The path analysis showed a greater direct effect of the number of fruits per plant with and without the presence of silicon. Fruit weight, number of fruits per plant, and agricultural yield increased linearly with increasing silicon dose. Implications: The best adjustment of fruit weight both in the presence and absence of silicon when it is related to the yield corresponded to a non-linear model, specifically to a Hill sigmoidal function. Conclusion: Through multiple linear regression, it was shown that both yield component variables, i.e. fruit weight and number of fruits per plant, have a greater influence on agricultural yield when fertilized with silicon.

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“…The harm caused by the massive use of fungicides has begun to attract people's attention. Fungicides have genotoxicity and cytotoxicity, 18 which can lead to apoptosis 19 and affect immune cells. 20 Procymidone (PRO) [N- (3,5-dichlorophenyl)-1,2-dimethylcyclopropane dicarboximide] is a dicarboximide fungicide.…”

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confidence: 99%

Effects of maternal exposure to procymidone on hepatic metabolism in the offspring of mice

Wang

Jin

et al. 2023

Environmental Toxicology

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As an effective fungicide widely used in agricultural production, the excessive procymidone (PRO) residue has been detected in the environment and food. Our previous study demonstrated that PRO could destroy the intestinal barrier in mice and has a joint toxic effect. To explore the cross-generational impact of maternal exposure, 10-week-old C57BL/6 female mice were orally administrated to 10 and 100 mg/kg body weight/day of PRO during pregnancy and lactation. The offspring obtained nutrients from the maternal through the placenta and breast milk, and PRO residues were detected in the liver, intestine, and feces of F1 generation. Fecal examination found that the residual PRO had been completely metabolized when the offspring mice grew to 35 days. The drug residue of F1 generation male mice was higher than that of female mice. We attributed this result to the difference in cytochrome P450 (CYP450) enzyme expression between male and female mice. The transcriptional levels of CYP1A1, CYP1A2, CYP2D9, and CYP3A4, and CYP450 protein expression levels, were higher in female mice. Furthermore, targeted MS of plasma revealed abnormal amino acid levels. In addition, PRO-induced hepatic metabolite changes in F0 and F1-7w mice. KEGG pathway analysis further showed that PRO jointly changed the amino acid biosynthesis pathway of the maternal and offspring. In summary, these results indicated that maternal exposure to PRO during a special period would interfere with self metabolism, and offspring will also have metabolic disorders.

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DNA damage and cell apoptosis induced by fungicide difenoconazole in mouse mononuclear macrophage RAW264.7

Cited by 11 publications

References 51 publications

Protective Effect of Rutin on Triethylene Glycol Dimethacrylate-Induced Toxicity through the Inhibition of Caspase Activation and Reactive Oxygen Species Generation in Macrophages

Protective Effect of Rutin on Triethylene Glycol Dimethacrylate-Induced Toxicity through the Inhibition of Caspase Activation and Reactive Oxygen Species Generation in Macrophages

Relations Between the Mass of the Fruits, the Number of Fruits Per Plant and the Agricultural Yield in Pepper, With Increasing Silicon Doses

Effects of maternal exposure to procymidone on hepatic metabolism in the offspring of mice

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