<scp>DNA</scp> barcoding of tuberous Orchidoideae: a resource for identification of orchids used in Salep

Ghorbani, Abdolbaset; Gravendeel, Barbara; Selliah, Sugirthini; Zarré, Shahin; Boer, Hugo de

doi:10.1111/1755-0998.12615

Cited by 37 publications

(33 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All collected individuals belonged to three genera ( Anacamptis, Himantoglossum, and Ophrys ). The high frequency of harvested Himantoglossum individuals (39.5%), and the low frequency of Orchis (0%) within our sample of harvested individuals, contrasts with a previous study from Iran (Ghorbani et al., ). There are several, mutually nonexclusive explanations for this discrepancy.…”

Section: Discussioncontrasting

confidence: 99%

“…Harvesting tubers of terrestrial orchids to obtain a hot winter beverage (“ salep” ) or a special type of ice cream (“ salepi dondurma ”) is a century‐old, widespread practice in Turkey (Sezik, ,b; Tamer, Karaman, & Copur, ) and the Balkans (Kreziou, de Boer, & Gravendeel, ; Matović, Nikolić, Đelić, & Marković, ) and is recently booming in Iran as a result of increased demands from Turkey (Ghorbani, Gravendeel, Naghibi, & de Boer, ; Ghorbani, Gravendeel, Selliah, Zarré, & de Boer, ).…”

Section: Introductionmentioning

confidence: 99%

“…The increased wealth of the middle class, and the growing western export resulted in increased demand for salep, and its price also increased substantially (Ghorbani et al., , ). As a consequence, the unsustainable collection of tubers threatens wild orchid populations (cultivating terrestrial orchids for salep production is not known).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Turkish graveyards as refuges for orchids against tuber harvest

Molnár

Nagy

Löki

et al. 2017

Ecology and Evolution

View full text Add to dashboard Cite

Harvest of orchid tubers for salep production is widespread in southwestern Asia and the Balkans and constitutes a major conservation risk for wild orchid populations. Synanthropic habitats, such as graveyards, are important refuges for orchids and other organisms and could offer protection from salep harvesting because of their special cultural role. However, little is known about the occurrence and factors influencing harvesting of salep in graveyards. During field surveys of 474 graveyards throughout Turkey, we observed 333 graveyards with orchids, 311 graveyards with tuberous orchids, and salep harvest in 14 graveyards. Altogether, 530 individuals of 17 orchid species were collected, representing 9% of the individuals recorded. Harvesting intensity was relatively low, and populations were usually not wholly destroyed. However, some species were clearly more affected than others. Salep harvesting risk of orchid species was significantly associated with flowering time, with early‐flowering species being more affected. A marginally significant positive relationship between harvesting risk and species‐specific tuber size was also detected. Our data suggest that graveyards might offer some protection against salep harvesting in Turkey, but they also show that some orchid taxa are much more affected than others. Overall, our observations add more weight to the conservation value of these special habitats.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Turkish graveyards as refuges for orchids against tuber harvest

Molnár

Nagy

Löki

et al. 2017

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

“…Setting the clustering threshold to 100%, on the other hand, likely resulted in more than one MOTU being formed for a single taxonomic species as a result of infraspecific variation and/or sequencing errors between reads. Here we followed previous studies in using a 99% clustering threshold (Ghorbani et al, 2016; Raclariu et al, 2017; Veldman et al, 2017). Furthermore, to limit the impact of sequencing errors, which are known to affect the Ion Torrent sequencing platform (Loman et al, 2012; Salipante et al, 2014) and which could lead to the formation of false MOTUs, we used only the clusters that contained a minimum 10 reads.…”

Section: Resultsmentioning

confidence: 99%

Veronica officinalis Product Authentication Using DNA Metabarcoding and HPLC-MS Reveals Widespread Adulteration with Veronica chamaedrys

et al. 2017

Self Cite

View full text Add to dashboard Cite

Studying herbal products derived from local and traditional knowledge and their value chains is one of the main challenges in ethnopharmacology. The majority of these products have a long history of use, but non-harmonized trade and differences in regulatory policies between countries impact their value chains and lead to concerns over product efficacy, safety and quality. Veronica officinalis L. (common speedwell), a member of Plantaginaceae family, has a long history of use in European traditional medicine, mainly in central eastern Europe and the Balkans. However, no specified control tests are available either to establish the quality of derived herbal products or for the discrimination of its most common substitute, V. chamaedrys L. (germander speedwell). In this study, we use DNA metabarcoding and high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) to authenticate sixteen V. officinalis herbal products and compare the potential of the two approaches to detect substitution, adulteration and the use of unreported constituents. HPLC-MS showed high resolution in detecting phytochemical target compounds, but did not enable detection of specific plant species in the products. DNA metabarcoding detected V. officinalis in only 15% of the products, whereas it detected V. chamaedrys in 62% of the products. The results confirm that DNA metabarcoding can be used to test for the presence of Veronica species, and detect substitution and/or admixture of other Veronica species, as well as simultaneously detect all other species present. Our results confirm that none of the herbal products contained exactly the species listed on the label, and all included substitutes, contaminants or fillers. This study highlights the need for authentication of raw herbals along the value chain of these products. An integrative methodology can assess both the quality of herbal products in terms of target compound concentrations and species composition, as well as admixture and substitution with other chemical compounds and plants.

show abstract

“…In Turkey, tubers of the terrestrial orchids have been collected for centuries, and used for making a hot winter beverage (salep) and ice-cream (salepi dondurma), and are still used for those purposes [13,14]. Salep collection is considered one of the most important threats to orchids in the Balkans [15], in the Southwestern Asia [16,17], and especially in Turkey [13,[18][19][20][21][22][23][24]. Germany is one of the major importers of salep in Europe: the number of excavated, exported orchid individuals just for salep in Germany is approximately 3 or 4 million individuals per year [19].…”

Section: Introductionmentioning

confidence: 99%

Using traditional ecological knowledge in discovery of rare plants: a case study from Turkey

et al. 2017

View full text Add to dashboard Cite

Sustainable (and adaptive) management of natural resources is usually based on long term local experiences with nature. Local traditional communities often possess rich ecological knowledge connected to nature and traditional resource use and management. This knowledge can provide unexpected new information for researchers, and show new opportunities and ways for professionals in conserving rare and threatened species.We found significant new populations of the rare Ophrys lesbis in a private area next to the settlement of Çamlık, Muğla, and Orchis punctulata in the graveyard of Kadılar, Antalya with the help of local rural people. We firstly report the replanting of some orchid species (Orchis papilionacea, O. italica, and Barlia robertiana) in kitchen gardens of Çamlık and Bayır, in Muğla Province.The presence of significant orchid populations (e.g., the biggest ever found for Ophrys lesbis) in an area, where local owners have been actively harvesting salep from year to year for decades suggests that the moderate salep harvesting can be sustainable for long run. Based on our observations, Turkish salep harvesters can help botanists and conservationists find new locations of rare threatened orchid populations, and therefore indirectly help in conserve these populations.

show abstract

DNA barcoding of tuberous Orchidoideae: a resource for identification of orchids used in Salep

Cited by 37 publications

References 73 publications

Turkish graveyards as refuges for orchids against tuber harvest

Turkish graveyards as refuges for orchids against tuber harvest

Veronica officinalis Product Authentication Using DNA Metabarcoding and HPLC-MS Reveals Widespread Adulteration with Veronica chamaedrys

Using traditional ecological knowledge in discovery of rare plants: a case study from Turkey

Contact Info

Product

Resources

About