<scp>DNA</scp> barcoding and morphological identification of neotropical ichthyoplankton from the Upper Paraná and São Francisco

Becker, RA; Sales, Naiara Guimarães; Santos, Gilmar B.; Carvalho, DC

doi:10.1111/jfb.12707

Cited by 53 publications

(45 citation statements)

References 28 publications

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“…The use of more modern techniques, particularly biotelemetry, in recent years has made it possible to more accurately monitor migratory movements of fishes in different river basins across the continent (Godinho & Kynard, 2008;Hahn, 2012;Suzuki, Dunham, Silva, Alves, & Pompeu, 2017). Because most Neotropical migratory fish species share many similarities in egg and larval morphology, genetic analyses may be useful for identifying their ichthyoplankton (Becker, Sales, Santos, Santos, & Carvalho, 2015). Because most Neotropical migratory fish species share many similarities in egg and larval morphology, genetic analyses may be useful for identifying their ichthyoplankton (Becker, Sales, Santos, Santos, & Carvalho, 2015).…”

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“…Egg and larval sampling also has been increasingly used in Neotropical river basins for determining potential spawning sites (Nakatani et al, 2001;Reynalte-Tataje et al, 2012;Suzuki & Pompeu, 2016). Because most Neotropical migratory fish species share many similarities in egg and larval morphology, genetic analyses may be useful for identifying their ichthyoplankton (Becker, Sales, Santos, Santos, & Carvalho, 2015). The combination of these different techniques-biotelemetry, egg and larval sampling, and genetics-can provide an integrated view of migratory and reproductive dynamics of the targeted species.…”

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The critical importance of an undammed river segment to the reproductive cycle of a migratory Neotropical fish

Lopes

Pompeu

Alves

et al. 2018

Ecology of Freshwater Fish

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Biotelemetry, ichthyoplankton and genetic data can provide detailed information about the migratory dynamics and reproductive cycle of freshwater fishes. However, few studies have combined these techniques in Neotropical systems. The objective of this study was to examine the migratory and reproductive dynamics of Prochilodus costatus in the São Francisco River watershed, south‐east Brazil, by comparing the ecological importance of two rivers to the species, an undammed segment of the São Francisco River and a dammed segment of one of its main tributaries, the Pará River. In total, 215 fish were radio‐tagged over three years (2014–16). Eggs and larvae were sampled at seven locations and analysed by PCR to identify Prochilodus spp. ichthyoplankton. Most radio‐tagged individuals (97%) used the undammed segment of the São Francisco River as spawning migration route, even those captured and released in the Pará River. Fish migrated to spawn from late September to late November with the arrival of the rains and returned to feeding sites from December to May after spawning. The highest densities of fish eggs and larvae were recorded in the upper reaches of the São Francisco River during months of peak river discharge. Returning fish showed high fidelity to sites occupied before spawning migration. Fish spent roughly 71% of the year at feeding sites, 25% at spawning sites and 4% moving between them. This study provides novel information about the migratory dynamics of Neotropical fishes and underscores the key role of undammed river segments for the conservation of Neotropical migratory fish species.

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The critical importance of an undammed river segment to the reproductive cycle of a migratory Neotropical fish

Lopes

Pompeu

Alves

et al. 2018

Ecology of Freshwater Fish

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“…Analyses on 254 species of fish from the Upper Parana River Basin in Brazil correctly identified 252 species using their barcode sequences, including a large number of closely related species [179]. Moreover, comparative analyses using traditional morphological taxonomy and DNA barcoding of Neotropical ichthyoplankton from the Upper Paraná and São Francisco Rivers showed no conflicting results between the two techniques [180].…”

Section: Identification Of Neotropical Cryptic Speciesmentioning

confidence: 91%

Genetic Applications in the Conservation of Neotropical Freshwater Fish

Mastrochirico-Filho¹,

Freitas²,

Ariede³

et al. 2018

Biological Resources of Water

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Neotropical fish correspond to approximately 30% of all fish species worldwide. The diversity of fish species found in Neotropical basins reflects variations in life-history strategies and exhibition of particular morphological, physiological and ecological attributes. These attributes are mainly related to different forms of feeding, life maintenance and reproduction. Today, fish populations are being threatened by anthropogenic actions that are having a visible impact on the natural state of continental aquatic ecosystems. The main causes are overfishing, non-native species introduction, reservoir-dam systems, mining, pollution and deforestation. The biology and population dynamics of the species are still unclear due to lack of research. Genetic tools can be useful resources for the conservation of Neotropical fish species in several ways. Molecular genetic markers are considered powerful tools to identify cryptic and hybrid fish and also allow the evaluation of the genetic variability and structure of populations of Neotropical ichthyofauna. Several analyses of molecular markers have been performed on Neotropical fish, including allozyme analysis, restriction fragment length polymorphisms in regions of DNA (RFLP), randomly amplified polymorphic DNA (AFLP), randomly amplified polymorphic DNA (RAPD), microsatellites, single nucleotide polymorphisms (SNPs) and mitochondrial DNA (mtDNA) markers. In order to analyse a high number of markers, next generation sequencing has allowed researchers to generate a large amount of genomic information that can be applied to the conservation of Neotropical fish.Keywords: molecular markers, genetic conservation, Neotropical ichthyofauna, overfishing, dam © 2018 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Rivers of the Neotropical regionThe distribution of freshwater fish around the world was mediated by historical climatic and geological events at different time points. Today, each global region has distinct patterns of distribution due to physical barriers obstructing species dispersion, representing different tolerances to environmental variables [1]. The tropics of the American continent are well known for their high biodiversity. This is due to habitat heterogeneity and a complex geological history. The Neotropical region is a biogeographic region that comprises Central America (including the southern part of Mexico and the peninsula of Baja California), the south of Florida, the Caribbean and South America. The origin and evolution of the Neotropical region arose through a process of synergism between its fauna that experienced local rainfall variations and gradual climate change resulting in a mosaic of habitats controlled by river migrations, sea-level fluctuations, local dryness and local uplifts [2,3].Regional geo...

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“…Larval fish have been successfully identified using DNA barcoding in Australia (Pegg et al, 2006), among coral reefs in the Indo-Pacific (Hubert et al, 2010), along the Caribbean coast of Panama (Victor et al, 2009), and along the Yucatan Peninsula, Mexico (Valdez-Moreno et al, 2010). However, few empirical studies have been undertaken to compare the effectiveness and efficiency of visual and DNA barcoding identification techniques (e.g., Ko et al, 2013;Becker et al, 2015;Puncher et al, 2015).…”

Section: Introductionmentioning

confidence: 98%

Increased taxonomic resolution of Laurentian Great Lakes ichthyoplankton through DNA barcoding: A case study comparison against visual identification of larval fishes from Stokes Bay, Lake Huron

Overdyk

Holm

Crawford

et al. 2016

Journal of Great Lakes Research

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DNA barcoding and morphological identification of neotropical ichthyoplankton from the Upper Paraná and São Francisco

Cited by 53 publications

References 28 publications

The critical importance of an undammed river segment to the reproductive cycle of a migratory Neotropical fish

The critical importance of an undammed river segment to the reproductive cycle of a migratory Neotropical fish

Genetic Applications in the Conservation of Neotropical Freshwater Fish

Increased taxonomic resolution of Laurentian Great Lakes ichthyoplankton through DNA barcoding: A case study comparison against visual identification of larval fishes from Stokes Bay, Lake Huron

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