<scp>DELLA</scp> proteins physically interact with <scp>CONSTANS</scp> to regulate flowering under long days in <i>Arabidopsis</i>

Xu, Feng; Li, Ting; Xu, Pengbo; Li, Ling; Du, Shanshan; Li, Hongli; Yang, Huey‐Lang

doi:10.1002/1873-3468.12076

Cited by 92 publications

(74 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2B). In agreement with our results, Xu et al (2016) showed the DELLA protein GAI physically interacts with CO in yeast cells and binds to CO in vitro.…”

Section: Della Physically Interact With Cosupporting

confidence: 93%

“…Consistent with previous studies, we also found that CO transcription and CO protein abundance were not regulated by the GA pathway (Supplemental Fig. S1; Galvão et al, 2012;Xu et al, 2016). Recently, several transcription factors have been found to physically interact with DELLA repressors to regulate various GA-mediated physiological processes (Yu et al, 2012;Davière et al, 2008;de Lucas et al, 2008;Feng et al, 2008;Hong et al, 2012;Wild et al, 2012).…”

Section: Della Physically Interact With Cosupporting

confidence: 91%

“…Very recently, Xu et al (2016) reported that DELLA proteins physically interact with CO, indicating a direct association between the photoperiod and GA pathways. However, the biological significance of the DELLA-CO physical interactions remains largely unclear.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The DELLA-CONSTANS Transcription Factor Cascade Integrates Gibberellic Acid and Photoperiod Signaling to Regulate Flowering

et al. 2016

View full text Add to dashboard Cite

Gibberellin (GA) and photoperiod pathways have recently been demonstrated to collaboratively modulate flowering under long days (LDs). However, the molecular mechanisms underlying this collaboration remain largely unclear. In this study, we found that GA-induced expression of FLOWERING LOCUS T (FT) under LDs was dependent on CONSTANS (CO), a critical transcription factor positively involved in photoperiod signaling. Mechanistic investigation revealed that DELLA proteins, a group of crucial repressors in GA signaling, physically interacted with CO. The DELLA-CO interactions repressed the transcriptional function of CO protein. Genetic analysis demonstrated that CO acts downstream of DELLA proteins to regulate flowering. Disruption of CO rescued the earlier flowering phenotype of the gai-t6 rga-t2 rgl1-1 rgl2-1 mutant (dellap), while a gain-of-function mutation in GA INSENSITIVE (GAI, a member of the DELLA gene) repressed the earlier flowering phenotype of CO-overexpressing plants. In addition, the accumulation of DELLA proteins and mRNAs was rhythmic, and REPRESSOR OF GA1-3 protein was noticeably decreased in the long-day afternoon, a time when CO protein is abundant. Collectively, these results demonstrate that the DELLA-CO cascade inhibits CO/FT-mediated flowering under LDs, which thus provide evidence to directly integrate GA and photoperiod signaling to synergistically modulate flowering under LDs.

show abstract

“…2B). In agreement with our results, Xu et al (2016) showed the DELLA protein GAI physically interacts with CO in yeast cells and binds to CO in vitro.…”

Section: Della Physically Interact With Cosupporting

confidence: 93%

Section: Della Physically Interact With Cosupporting

confidence: 91%

See 1 more Smart Citation

The DELLA-CONSTANS Transcription Factor Cascade Integrates Gibberellic Acid and Photoperiod Signaling to Regulate Flowering

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Mutations in GA signaling or the depletion of active GA pools cause late flowering with lower expression of FT in long days. It was recently demonstrated that GA signaling can be integrated into the photoperiodic pathway partly through physical interaction of DELLA with CO (Xu et al, 2016;Yu et al, 2016;Fig. 2).…”

Section: Transcriptional Regulation Of Ftmentioning

confidence: 45%

“…Co-expression of DELLA protein attenuates CO-dependent activation of the FT promoter in Arabidopsis protoplasts . This suppression could be attained by dosage-dependent inhibition of DELLA proteins on CO-NF-Y complex formation, as an increasing amount of DELLA proteins results in a decreased amount of CO and NF-Y interaction (Xu et al, 2016). The expression profile of REPRESSOR OF GA1-3 protein, a member of the DELLA protein family, diurnally oscillates with a trough in the afternoon when CO induces FT expression.…”

Section: Transcriptional Regulation Of Ftmentioning

confidence: 99%

Circadian Clock and Photoperiodic Flowering in Arabidopsis: CONSTANS Is a Hub for Signal Integration

2016

View full text Add to dashboard Cite

Plants sense changes in day length (= photoperiod) as a reliable seasonal cue to regulate important developmental transitions such as flowering. Integration of various external light information into the circadian clock-controlled mechanisms enables plants to precisely measure photoperiod changes in the surrounding environment. The core mechanism of photoperiodic measurement is regulation of CONSTANS (CO) activity, which takes place in phloem companion cells in leaves. Until recently, it remained unclear whether plants possess specific variations of the clock for this regulation. Now it is known that a specific circadian timing mechanism in the vascular tissue is essential for photoperiodic flowering. In addition to spatial tissue-specific regulation, temporal regulation of CO activity is also important. The identification and characterization of multiple regulators that physically interact with CO and influence its function in the morning in long days are two recent advances in photoperiodic regulation of flowering time. It seems that CO acts as a network hub to integrate various external and internal signals into the photoperiodic flowering pathway. CO regulates the amount of FLOWERING LOCUS T (FT) transcripts and FT protein moves from companion cells of leaf phloem to the shoot apical meristem. The protein that helps long-distance transport of FT protein was also identified recently. Here, we introduce recent advances in tissue-specific variations of the circadian clock and the emerging picture of the intricate connections of transcriptional regulators through CO in the morning, which all facilitate plants knowing when to flower.Properly timing the floral transition is crucial for reproductive success. It can also influence the early development of offspring. To optimize this timing, plants constantly monitor changes in the surrounding environment. Among various environmental factors, observing changes in day length (= photoperiod) is the most reliable way for many organisms to know the specific time of year. Therefore, photoperiodic regulation is one of the major flowering time mechanisms and it has been studied since it was first reported in 1920 (Song et al., 2015). Arabidopsis (Arabidopsis thaliana), as it flowers mainly in spring, can sense lengthening days to induce flowering and became a suitable model to study photoperiodic flowering regulation. (Andrés and Coupland, 2012;Song et al., 2013;Pajoro et al., 2014;Shim and Imaizumi, 2015).In principle, photoperiodic flowering mechanisms can be divided into three parts: light input, circadian clock, and output. Light information is integrated into innate photoperiodic timing mechanisms governed by the circadian clock to induce genes that trigger flowering.

show abstract

DELLAs directed gibberellins responses orchestrate crop development: A brief review

et al. 2023

View full text Add to dashboard Cite

Gibberellins (GAs) are a pervasive group of diterpene phytohormones. They can dominate various aspects of plant development and modulate plant phenotypic plasticity by crosstalking with multiple phytohormones. Studies on GA signaling illustrate that master negative regulator DELLA proteins regulate GAs and nearly all phytohormones’ growth responses to sustain plant growth and defense under relentless biotic and abiotic stresses. The molecular mechanism of DELLA proteins in regulating GA‐sensitive growth in an integrated manner with multiple phytohormones became apparent recently. These studies describe the interaction of interaction DELLA proteins with a diverse group of transcriptional regulators and factors as well as nontranscriptional proteins in directing GA‐stimulated plant growth. In this review, we address the extensive recent work to delineate DELLA proteins and their interacting partners and summarize the latest evidence that DELLA proteins have a unique function in suppressing the GA‐related responses to sustain the tension among growth and defense.

show abstract

DELLA proteins physically interact with CONSTANS to regulate flowering under long days in Arabidopsis

Cited by 92 publications

References 39 publications

The DELLA-CONSTANS Transcription Factor Cascade Integrates Gibberellic Acid and Photoperiod Signaling to Regulate Flowering

The DELLA-CONSTANS Transcription Factor Cascade Integrates Gibberellic Acid and Photoperiod Signaling to Regulate Flowering

Circadian Clock and Photoperiodic Flowering in Arabidopsis: CONSTANS Is a Hub for Signal Integration

DELLAs directed gibberellins responses orchestrate crop development: A brief review

Contact Info

Product

Resources

About