<scp>d</scp>-α-tocopheryl Polyethylene Glycol 1000 Succinate: A View from FTICR MS and Tandem MS

Wei, Juan; Bristow, Anthony W. T.; McBride, Eileen; Kilgour, David P. A.; O’Connor, Peter B.

doi:10.1021/ac403195f

Cited by 16 publications

(28 citation statements)

References 35 publications

(59 reference statements)

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“…In EID, ions are irradiated with fast electrons (6-20 eV) in order to induce electronic excitation, form radical specials, and initiate dissociation. EID is usually applied to analysis of small molecules, which are not able to form multiple charges, such as glycosaminoglycan (Wolff et al, 2008), polyketides (Wills et al, 2012), amino acids (Lioe & O'Hair, 2007), non-ribosomal peptide (Wills & O'Connor, 2014), small pharmaceuticals (Mosely et al, 2011;Prakash et al, 2012), ions generated from matrix-assisted laser desorption/ionization (MALDI) sources (Nielsen et al, 2003), or larger molecules like chlorophyll-a (Wei et al, 2013) or even polymeric excipients (Wei et al, 2014).…”

Section: B Other Ion-electron Reaction-based Techniquesmentioning

confidence: 99%

Electron-based fragmentation methods in mass spectrometry: An overview

Volmer

2015

Mass Spec Rev

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Tandem mass spectrometry (MS/MS) provides detailed information for structural characterization of biomolecules. The combination of electron capture dissociation (ECD) techniques with Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) often provides unique ion-electron reactions and fragmentation channels in MS/MS. ECD is often a complimentary, sometimes even a superior tool to conventional MS/MS techniques. This article is aimed at providing a short overview of ECD-based fragmentation techniques (ExD) and optimization of ECD experiments for FTICR mass analyzers. Most importantly, it is meant to pique the interest of potential users for this exciting research field. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 36:4-15, 2017.

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Section: B Other Ion-electron Reaction-based Techniquesmentioning

confidence: 99%

Electron-based fragmentation methods in mass spectrometry: An overview

Volmer

2015

Mass Spec Rev

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“…Figure shows the results of the two-dimensional mass spectrometry analysis of TPGS performed with IRMPD and ECD as fragmentation techniques. The nomenclature for the fragmentation of TPGS is reported in this figure (top-right) as proposed in previous studies Figure a shows the 2D mass spectrum of TPGS with IRMPD as the fragmentation mode.…”

Section: Resultsmentioning

confidence: 99%

“…Two-dimensional analysis of the TPGS polymeric mixture in pure acetonitrile and Na 2 SO 4 with IRMPD and ECD as fragmentation techniques and proposed nomenclature . (a) IRMPD 2D mass spectrum with highlights on the most abundant fragmentations in the mixture.…”

Section: Resultsmentioning

confidence: 99%

“…Kaczorowska and Cooper reported the structural characterization of polyphosphoesters using infrared multiphoton dissociation (IRMPD) and electron capture dissociation (ECD) FTICR MS/MS . Finally, FTICR MS has been used to analyze the composition of different common polymeric excipients, such as Gelucire 44/14, Polysorbate 80, and TPGS . In particular for the last case, a detailed ECD and collisionally activated dissociation (CAD) MS/MS characterization of TPGS was performed, stepping up from an until-then underdeveloped structural characterization of synthetic polymers due to difficulties in their fragmentation and the necessity of different approaches for data analysis of different polymers types …”

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confidence: 99%

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Polymer Analysis in the Second Dimension: Preliminary Studies for the Characterization of Polymers with 2D MS

Floris

Vallotto

Chiron³

et al. 2017

Anal. Chem.

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Two-dimensional Fourier transform ion cyclotron resonance mass spectrometry (2D FTICR MS or 2D MS) allows direct correlation between precursor and fragment ions without isolation prior to fragmentation. The method has been optimized for the analysis of complex mixtures and used so far for the analysis of small molecules and peptides obtained by tryptic digestion of proteins and entire proteins. In this work, a 2D MS method is developed to characterize complex mixtures of polymers using infrared multiphoton decay (IRMPD) and electron capture dissociation (ECD) as fragmentation techniques, and D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS), Polysorbate 80, and poly(methyl methacrylate) (PMMA) as analytes. The use of 2D MS allowed generation of fragment m/z values for all the compounds in the mixture at once and allowed tandem mass spectrometry of species very close in m/z that would have been difficult to isolate with a quadrupole for standard MS/MS. Furthermore, the use of unique features of 2D MS such as the extraction of neutral-loss lines allowed the successful assignment of peaks from low abundant species that would have been more difficult with standard MS/MS. For all the samples, the amount of information obtained with 2D MS was comparable with what obtained with multiple 1D MS/MS experiments targeted on each individual component within each mixture but required a single experiment of about 20-40 min.

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“…79 Recently, Wei et al applied CID and ECD to study the composition and structure of TPGS mixtures as well as the influence of different metal cation adducts (K + , Na + , Ag + and Li + ) on fragmentation behavior. 80 It was found that dissociation of the charge carriers from the polymer mostly depended on the binding strength and that introduction of strongly binding adducts increased the fragmentation. For ECD, the loss of adducts seemed to correlate with the metal ion's reduction potential: metals with higher reduction potential were more effective for electron capture.…”

Section: Methodsmentioning

confidence: 99%

Structural analysis of small to medium-sized molecules by mass spectrometry after electron-ion fragmentation (ExD) reactions

Volmer

2016

Analyst

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Electron capture dissociation (ECD) is a tandem mass spectrometry (MS/MS) method that utilizes the interaction of ions and electrons. Its unique ability to preserve labile bonds distinguishes it from conventional threshold-based MS/MS methods, the most important of which is collision-induced dissociation (CID). During the last decade, ECD has opened up several new venues in protein analyses, for example top-down sequencing, identification of post-translational modifications, and characterization of protein-protein interactions. In recent years, a number of related dissociation techniques, so-called ExD techniques, particularly electron transfer dissociation (ETD), electron detachment dissociation (EDD), electron induced dissociation (EID), and negative electron transfer dissociation (NETD), have emerged and have extended the application range of ion-electron dissociations further. Importantly, ExD techniques have been applied beyond protein analyses, which is the focus of the current paper. This short introduction describes the application of ExD to small and medium-sized molecules and reviews important applications to natural products, biomedical compounds, synthetic molecules, crude oils, and environmental toxins.

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d-α-tocopheryl Polyethylene Glycol 1000 Succinate: A View from FTICR MS and Tandem MS

Cited by 16 publications

References 35 publications

Electron-based fragmentation methods in mass spectrometry: An overview

Electron-based fragmentation methods in mass spectrometry: An overview

Polymer Analysis in the Second Dimension: Preliminary Studies for the Characterization of Polymers with 2D MS

Structural analysis of small to medium-sized molecules by mass spectrometry after electron-ion fragmentation (ExD) reactions

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