<scp>D</scp>-GLYCERO-<scp>D</scp>-MANNO-HEPTOSE AS A COMPONENT OF LIPOPOLYSACCHARIDES FROM GRAM-NEGATIVE BACTERIA

Adams, G. A.; Quadling, C.; Perry, Malcolm B.

doi:10.1139/m67-210

Cited by 44 publications

(25 citation statements)

References 9 publications

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“…1). Previous studies (Adams, Quadling and Perry, 1967;Dmitriev et al, 1971) indicate that this peak might be the alditol acetate of D-glycero-D-mannoheptose. The alditol acetate derived from an authentic sample of D-glycero-D-mannoheptose gave a R glu value of 2.10.…”

Section: Studies On the Lipopolysaccharide (Lps)mentioning

confidence: 81%

Immunochemical investigations on lipopolysaccharides and acidic polysaccharides from serum-sensitive and serum-resistant strains of Escherichia coli isolated from urinary-tract infections

Taylor

1976

Journal of Medical Microbiology

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ESCHERICHIA COLI LIPOPOL YSACCHARIDE 407Sensitivity to bacteriophages. Acidic polysaccharides and LPS 0 side-chains are known to interfere with the attachment of rough-specific phages. The phage sensitivity of the bacterial strains was therefore determined with six phages (nos. FO, Br2, BrlO, Fpl, 6SR and C21) which are actively lytic against certain R forms of E. coli (Schmidt et al., 1969). Phages were propagated on suitable host strains and bacterial sensitivity was determined by applying drops of phage containing approximately 108 p.f.u. per ml on to surface-inoculated nutrientagar plates. The phages and their host strains were supplied by Dr G. Schmidt.Preparation of LPS and acidic polysaccharides. The bacteria were grown in 4 x 700-ml amounts of Nutrient Broth (Oxoid no. 2) in 2.5-litre conical flasks. The cultures were incubated in an orbital incubator (120 orbits per min.) at 37°C for 16 h, harvested by centrifugation (O' C), washed twice with 0.4 % NaCl and dried to constant weight over PzOS.Two batches of each strain were prepared. One was extracted with 45 % phenol at 68°C (Westphal and Jann, 1965) and the other with 0.9 % NaCl at 60°C as described by Jann et al.

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Section: Studies On the Lipopolysaccharide (Lps)mentioning

confidence: 81%

Immunochemical investigations on lipopolysaccharides and acidic polysaccharides from serum-sensitive and serum-resistant strains of Escherichia coli isolated from urinary-tract infections

Taylor

1976

Journal of Medical Microbiology

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“…The symbol rfaE has been proposed for mutants deficient in the addition of the proximal heptose unit and rfaF for those deficient in formation of the distal heptose unit (Makela & Stocker, 1969). Note that this usage does not imply that the affected loci specify corresponding heptosyl transferases, since the defects may be in biosynthesis of the unknown precursor or precursors of the heptose units of the LPS core, which may be of two different types (Adams, Quadling & Perry, 1967). Uncommon nucleotides have been detected in cell extracts of some of these mutants: UDPrhamnose in the rfaE mutant S L I I O~ (Ginsburg, 1966) and ADPmannitol in the rfizFmutants S L I I~I and ~~1 1 8 2 (Scher & Ginsburg, 1968).…”

Section: Discussionmentioning

confidence: 99%

Non-smooth Mutants of Salmonella typhimurium: Differentiation by Phage Sensitivity and Genetic Mapping

Wilkinson

Gemski

Stocker

1972

Journal of General Microbiology

296

231

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S U M M A R YNon-smooth mutants of Salmonella typhimurium strain L T~ with known lipopolysaccharide (LPS) defects were tested for sensitivity to smooth-specific phages (P22 and P22h and a newly isolated phage, 9NA, active on P22 lysogens); Felix 0 phage; and several rough-specific phages including C21. Smooth strains were sensitive only to the smooth-specific and Felix 0 phages. Six rfb mutants (unable to make 0 chains) were sensitive to Felix 0 and all the rough-specific phages except C21 (pattern R-sens). Of nine rfa mutants (presumed to have LPS core defects) four were R-sens, six were resistant to Felix 0 and some rough-specific phages (pattern R-res-I), and one was also resistant to phage Br2 (pattern R-res-a). The phage sensitivity of phosphomannoisomerase (prni) mutants was the same as that of rfb mutants, except that they were partly sensitive to P22h. UDPgalactose-epimerasenegative mutants were sensitive to C21 and various rough-specific phages including Br2 (pattern Epi-I). An rfc mutant (unable to polymerize 0 repeat units) was sensitive only to Felix 0 and P221 (pattern Zsr). A part-rough mutant of class D (with abnormally few 0 chains) was incompletely resistant to smooth-specific phages, resistant to Felix 0 but sensitive to all rough-specific phages except C21 (pattern D-I).Spontaneous and mutagen-induced non-smooth mutants were isolated from LT2 strains with appropriate markers by selection with Felix 0 and/or P22 phage. (One parent strain used was non-lysogenic for Fels 2, for which LT2 wild-type is lysogenic. Lysogeny for Fels 2 did not affect sensitivity to the other phages.) Some mutants gave new sensitivity patterns. Mutants of these and of previously unmapped classes were crossed with smooth Hfr strains. The rfc loci of two mutants and pmi loci of two others were located in the gal-trp segment. Three mutants of pattern R-sens yielded 0-specific hapten but mapped near his; they are believed to be unable to transfer 0 chains from antigen carrier lipid to the LPS core as a result of mutation at rfbT. Six mutants of pattern R-sens were smooth in cultural and serological properties; they mapped near his and are probably leaky rfb mutants. Many mutants had the class D part-rough phenotype, divisible by phage sensitivities into patterns D-I, D-2 and D-3. Mutants of all three classes mapped near xyl; they are likely to be rfa mutants, perhaps leaky, with LPS core defects which hinder but do not prevent attachment of 0 chains. Two classes were sensitive to C21 (Wilkinson & Stocker, 1968): rfaH mutants, of pattern Epi-I, unable to add the main-chain galactose unit of the core; and rfaG mutants, resistant to Br2 (pattern Epi-a), unable to add the proximal glucose unit. Both loci mapped in or near the strA-xyl-metA segment. Several non-smooth mutants did not grow in the presence of bile-salts. Three mutants (rfaF) made LPS deficient of the distal heptose unit; one mutant (rfaE) was unable to add the proximal heptose unit. Both these loci mapped in or near the strA-metA segment. I N T R O D U C T I O N...

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“…The trans relationship of the hydroxyl groups on C-5 and C-6 of the glycero-D-munno-heptose unequivocally defined the aldose as L-glycero-D-manno-heptose. This heptose is a well known constituent of the endotoxins extracted from Enterobacteriae [33] but by no means the only one identified in microorganisms [34] : in particular u-gfycevo-D-murzno-heptose has been shown to be present in the endotoxin of a great number of bacterial species, and in particular, in that of the genus Xanthomonas [35].…”

Section: Discussionmentioning

confidence: 99%

7-O-(2-Amino-2-deoxy-alpha-d-glucopyranosyl) l-glycero-d-manno-heptose. A Constituent of the Endotoxin of Bordetella pertussis

Chaby

Szabó

1976

Eur J Biochem

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Hydrolysis of the Bordetella pertussis endotoxin, extracted from both ‘phase I’ and ‘phase IV’ bacteria, with 4 M HCL for 1 h at 100°C, released the disaccharide named in the title; it was isolated by paper electrophoresis or by ion‐exchange chromatography in about 1 % yield (w/w). The structure of the heptose could be rigorously established by chemical degradation; the facts that the glucosaminidic linkage was hydrolysed by an enzyme preparation containing both, α and β‐N‐acetylglucosaminidase activities, whereas it was resistant to cleavage by pure β‐N‐acetyl‐glucosaminidase strongly support the assumption that the disaccharide contains an α‐d‐glucosaminide linkage.

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D-GLYCERO-D-MANNO-HEPTOSE AS A COMPONENT OF LIPOPOLYSACCHARIDES FROM GRAM-NEGATIVE BACTERIA

Cited by 44 publications

References 9 publications

Immunochemical investigations on lipopolysaccharides and acidic polysaccharides from serum-sensitive and serum-resistant strains of Escherichia coli isolated from urinary-tract infections

Immunochemical investigations on lipopolysaccharides and acidic polysaccharides from serum-sensitive and serum-resistant strains of Escherichia coli isolated from urinary-tract infections

Non-smooth Mutants of Salmonella typhimurium: Differentiation by Phage Sensitivity and Genetic Mapping

7-O-(2-Amino-2-deoxy-alpha-d-glucopyranosyl) l-glycero-d-manno-heptose. A Constituent of the Endotoxin of Bordetella pertussis

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