<scp>Cip2A</scp> modulates osteogenic differentiation via the <scp>ERK‐Runx2</scp> pathway in <scp>MG63</scp> cells

Son, Hyo-Eun; Jang, Won‐Gu

doi:10.1002/biof.1760

Cited by 7 publications

(1 citation statement)

References 44 publications

(51 reference statements)

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“… 37 Moreover, many studies have indicated that PP2A is a negative factor in osteoblast differentiation. 38 Consistent with the previous findings, PP2A activity was reduced in OM, SGMS1 overexpression further inhibited PP2A activity, and Cer treatment partially reversed SGMS1 inhibition, thereby reducing PP2A activity. Moreover, SGMS1 knockdown increased PP2A activity that was decreased by OM, and SM treatment again decreased PP2A activity.…”

Section: Discussionsupporting

confidence: 91%

SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway

Yang,

Luan,

Wang

et al. 2024

iScience

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Section: Discussionsupporting

confidence: 91%

SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway

Yang,

Luan,

Wang

et al. 2024

iScience

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Human osteoprogenitor cells obtained from traumatic heterotopic ossification samples showed enhanced osteogenic differentiation potential and ERK/hedgehog signaling than that from normal bone

Zhang

Sun

et al. 2022

IUBMB Life

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Traumatic heterotopic ossification (HO) refers to the abnormal ectopic osteogenesis following trauma, causing limb dysfunction and seriously lowering the life quality of patients. Aberrant osteogenic behavior of progenitor cells that ectopically accumulated within the soft tissues are believed to be responsible for HO formation. However, the detailed mechanism still remained to be clarified. Here in this study, we successfully isolated osteoprogenitors from human heterotopic ossification tissues (HO-ops) and identified their stemness and multi-directional differentiation potential. Using alkaline phosphatase staining together with alizarin red staining, we confirmed that the HO-ops in the heterotopic ossified tissues gained greater osteogenic potential than the normal human bone marrow mesenchymal stem cells (HBMSCs). RT-qPCR also indicated that HO-ops obtained more gene transcriptions of critical osteogenic determinators than HBMSCs. In addition, through Western blot, we proved that ERK signaling pathway and hedgehog signaling pathway were significantly activated in the HO-ops. When U0126 and cyclopamine were used to inhibit ERK signaling and hedgehog signaling respectively, the osteogenic potential of HO-ops decreased significantly. The hedgehog signaling and ERK signaling also showed cross-talk in HO-ops during osteogenic differentiation in HO-ops during osteogenic differentiation. The elevated ERK signaling and hedgehog signaling were further confirmed in the human traumatic HO

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KAP1 modulates osteogenic differentiation via the ERK/Runx2 cascade in vascular smooth muscle cells

et al. 2023

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Background Osteoblast phenotypic transition in vascular smooth muscle cells (VSMCs) has been unveiled as a common cause of vascular calci cation (VC). Krüppel-Associated Box (KRAB)-Associated Protein 1(KAP1) is a transcriptional corepressor that modulates various intracellular pathological processes from gene expression to DNA repair to signal transduction. However, the function and mechanism of KAP1 on the osteoblastic differentiation of VSMCs have not been evaluated yet. Methods and resultsWe demonstrate that the expression of KAP1 in VSMCs is signi cantly enhanced in vivo and in vitro calci cation models. Downregulating the expression of KAP1 suppresses the osteoblast phenotypic transition of VSMCs, which is indicated by a decrease in the expression of osteoblast marker collagenase type I (COL I) and an increase in the expression of VSMC marker α-smooth muscle actin (α-SMA). Conversely, exogenous overexpression of KAP1 could promote osteoblast phenotypic transition of VSMCs. Moreover, KAP1 upregulated the expression of RUNX family transcription factor 2 (Runx2), an inducer of osteoblast that positively regulates many osteoblast-related genes, such as COL I. Evaluation of the potential mechanism demonstrated that KAP1 promoted osteoblast phenotypic transition of VSMCs by activating the extracellular regulated protein kinases (ERK) signaling pathway, which could activate Runx2. In support of this nding, KAP1-induced cell osteoblast phenotypic transition is abolished by treatment with PD0325901, a speci c ERK inhibitor.Conclusions The present study suggested that KAP1 participated in the osteoblast differentiation of VSMCs via the ERK/Runx2 cascade and served as a potential diagnostics and therapeutics target for vascular calci cation.

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Cip2A modulates osteogenic differentiation via the ERK‐Runx2 pathway in MG63 cells

Cited by 7 publications

References 44 publications

SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway

SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway

Human osteoprogenitor cells obtained from traumatic heterotopic ossification samples showed enhanced osteogenic differentiation potential and ERK/hedgehog signaling than that from normal bone

KAP1 modulates osteogenic differentiation via the ERK/Runx2 cascade in vascular smooth muscle cells

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