CHD5 gene (rs9434741) might be a genetic risk factor for infertility in non‐obstructive azoospermia and severe oligozoospermia

Abstract

Inability to conceive is one of the health concerns. Chromodomain helicase DNA-binding protein5 (CHD5) gene is a major regulator in the replacement of histone proteins with protamines, the chromatin remodelling in spermatogenesis process. Thus, functional SNPs in this gene can disrupt sperm development. This study aimed to investigate the relationship between CHD5 polymorphism (rs9434741) and male infertility. This case-control study was conducted on 103 infertile and 121 fertile men. CHD5 polymorphism rs9434741 was tested using T-ARMS-PCR to investigate its association with male infertility. The presence of G allele caused 1.52 fold increase (OR = 1.52, 95% CI = 1.09-2.31 and p = 0.019) in infertility susceptibility in the patient group. GG genotype and (AG+GG) were significantly related to the increased risk of infertility (OR = 3.13, 95% CI = 1.26-7.76, p = 0.013; OR = 2.72, 95% CI = 1.35-5.47), respectively. Significant differences were observed between genotypes in NOA and SO groups compared to the control group (p = 0.029). Sperm count and total motility were significantly different among three genotypes in infertile men and the control group (p < 0.001). Analysis of genotypes and alleles frequency indicated statistically significant differences between the patient and control groups (p < 0.05). This study showed that CHD5 polymorphism (rs9434741) could be associated with the risk of male infertility. It is recommended to conduct further studies on different populations.

An artificial neural network model to diagnose non-obstructive azoospermia based on RNA-binding protein-related genes

“…Therefore, the surgical removal of the blockage and the testicular puncture are valid in OA, while the current treatment strategies for NOA often fail [ 4 ]. Seeking the underlying biological mechanisms of NOA has attracted increasing attention in the past decades, and some critical genes were uncovered, such as VASA [ 5 ], CHD5 [ 6 ], and MEIOB [ 7 ], providing possible therapeutic targets. Nevertheless, our understandings of the genetic alternation of NOA remain limited.…”

Reply to the Letter to the Editor “Considerations for incorporating circulating miRNA into reproductive medicine—A letter regarding Naeimi et al. study”

“…2 Our previous study showed that the distribution of CHD5 genotypes and frequency of alleles were statistically significant between infertile men and control groups and proposed that CHD5 polymorphism (rs9434741) could be associated with the risk of male infertility. 3 We discussed that the CHD5 gene plays a role in sperm density and there was shown a close connection between miR-211 and the CHD5 gene expression. Thus, we decided to study miR-211 expression and compare it between the two groups.We also mentioned that many miRNAs in testicular tissue and semen samples could be investigated.…”