<scp>CD</scp>44<sup>high</sup>/<scp>ALDH</scp>1<sup>high</sup> head and neck squamous cell carcinoma cells exhibit mesenchymal characteristics and <scp>GSK</scp>3β‐dependent cancer stem cell properties

Seino, Sayaka; Shigeishi, Hideo; Hashikata, Miho; Higashikawa, Koichiro; Tobiume, Kei; Uetsuki, Ryo; Ishida, Yasushi; Sasaki, Kazuki; Naruse, Takako; Rahman, MK; Ono, Shigehiro; Simasue, Hiroshi; Ohta, Kouji; Sugiyama, Masaru; Takechi, Masaaki

doi:10.1111/jop.12348

Cited by 29 publications

(50 citation statements)

References 46 publications

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“…Our early study and other groups had reported that Lin28B can promote cell malignancy, 10,35 cancer progression, and inflammation-induced carcinogenesis, 33 BMI-1 with oral carcinogenesis, 13 and ALDH1+ cells show cancer stem cell characteristics. 14,15,17 Considering the above mentioned points, we speculated potential relation of TRAF1 with stem cell subpopulations in oral cancer, which needs to be investigated further by molecular mechanism studies.…”

Section: Discussionmentioning

confidence: 99%

“…33 Stem cell markers, including BMI-1, ALDH1, and Lin28B, are associated with head and neck tumorigenesis. 6,7,17 About 40.9% of oral leukoplakia patients with BMI-1 positivity develop oral cancer. 12 ALDH1-high cancer cells exhibit a high tumor sphere-forming ability.…”

Section: Discussionmentioning

confidence: 99%

“…12 ALDH1-high cancer cells exhibit a high tumor sphere-forming ability. 17 The reprogramming factor Lin28B promotes cancer stem-like properties in oral cancer. 7 Inflammation site cytokines, such as transforming growth factor (TGF)-β, leukemia inhibitory factor, (LIF), basic fibroblast growth factor (b-FGF), and interleukin (IL)-6, can directly or indirectly upregulate the stem cell marker characteristics, including Lin28, Sox2, Nanog, and Oct4.…”

Section: Discussionmentioning

confidence: 99%

“…[14][15][16] Current studies have shown that ALDH1 is a cancer stem cell marker in lung, pancreatic, prostate, and head and neck cancers. 17 To date, no study has performed a comprehensive analysis of the expression of TRAF1 in human OSCC and its associations with Lin28B, BMI-1, and ALDH1 expression in OSCC and patients' clinical outcome. In this study, we examined the expression of TRAF1 in OSCC and its associations with Lin28B, ALDH1, and BMI-1 in OSCC by employing tissue microarrays.…”

Section: Introductionmentioning

confidence: 99%

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Expression and associations of TRAF1, BMI-1, ALDH1, and Lin28B in oral squamous cell carcinoma

et al. 2017

Tumour Biol.

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Tumor necrosis factor receptor-associated factor 1, an adaptor protein of tumor necrosis factor 2, is involved in classical nuclear factor (NF)-κB activation and lymphocyte recruitment. However, less is known about the expression and association of tumor necrosis factor receptor-associated factor 1 with cancer stem cell markers in oral squamous cell carcinoma. This study aimed to investigate the expression of tumor necrosis factor receptor-associated factor 1 and stem cell characteristic markers (lin28 homolog B, B cell-specific Moloney murine leukemia virus integration site 1, and aldehyde dehydrogenase 1) in oral squamous cell carcinoma and analyze their relations. Paraffin-embedded tissues of 78 oral squamous cell carcinomas, 39 normal oral mucosa, and 12 oral dysplasia tissues were employed in tissue microarrays, and the expression of tumor necrosis factor receptor-associated factor 1, B cell-specific Moloney murine leukemia virus integration site 1, aldehyde dehydrogenase 1, and lin28 homolog B was measured by immunohistostaining and digital pathological analysis. The expression of tumor necrosis factor receptor-associated factor 1 was higher in the oral squamous cell carcinoma group as compared with the expression in the oral mucosa (p < 0.01) and oral dysplasia (p < 0.001) groups. In addition, the expression of tumor necrosis factor receptor-associated factor 1 was associated with those of B cell-specific Moloney murine leukemia virus integration site 1, aldehyde dehydrogenase 1, and lin28 homolog B (p = 0.032, r 2 = 0.109; p < 0.0001, r 2 = 0.64; and p < 0.001, r 2 = 0.16) in oral squamous cell carcinoma. The patient survival rate was lower in the highly expressed tumor necrosis factor receptor-associated factor 1 group, although the difference was not significant. The clustering analysis showed that tumor necrosis factor receptor-associated factor 1 was most related to aldehyde dehydrogenase 1. These findings suggest that tumor necrosis factor receptor-associated factor 1 has potential direct/indirect regulations with the cancer stem cell markers in oral squamous cell carcinoma, which may help in further analysis of the cancer stem cell characteristics.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression and associations of TRAF1, BMI-1, ALDH1, and Lin28B in oral squamous cell carcinoma

et al. 2017

Tumour Biol.

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“…These cells are present in HNSCC, and overexpress CD44 and aldehyde dehydrogenase (ALDH) proteins, which are currently regarded as HNSCC CSCs' markers [127][128][129]. Moncharmont and colleagues reported that consecutive cell sorting was performed to isolate SQ20B/ CSCs from SQ20B parental population utilizing Side Population (SP).…”

Section: Carbon Ion Irradiation Counteracts Cancer Stem Cells' Migratmentioning

confidence: 99%

The impact of melatonin and carbon ion irradiation on cancer stem cells

Liu¹,

Reíter²

2017

Nucl Med Biomed Imaging

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Aim: Cancer stem cells (CSCs) exhibited an excessive migratory and invasive potential. Melatonin may inhibit multiple crucial signals associated with tumor stem cell self-renewal, viability, invasiveness, tumor growth, and therapy resistance. Carbon ion irradiation may be a promising therapeutic modality in both non-stem-like and stem-like tumor cells in contrast to photon irradiation. A comprehensive understanding of the mechanisms and molecular pathways associated with invasive properties of CSCs is essential in developing novel treatment options for cancer therapy that target CSCs. Materials and methods:A systematic review of the existing literature was conducted using the following search terms: 'melatonin', 'X-ray irradiation', 'charged particle irradiation', 'carbon ion irradiation', 'cancer stem cells', 'tumor-initiating cells' and 'cancer stem-like cells'. The search used PubMed and spanned the period from January 2000 to December 2016. Conclusion:Cancer stem cells possess the capacity of self-renewal and pluripotency, generating all cells within a tumor, and are responsible for tumor growth, therapy resistance and metastasis. Melatonin attenuated AKT activation, EZH2 S21 phosphorylation, EZH2-STAT3 interactions and altered histone modifications to reduce tumor initiation and propagation of brain tumor stem cells (BTSC). Melatonin increases the efficacy of chemotherapeutic agents, targeting both the tumor bulk and BTSCs through the regulation of the expression and function of the ABCG2/BCRP transporter by inducing the methylation of its promoter. Melatonin treatment induced cell death with ultrastructural characteristics of autophagy. Breast cancer stem cells (BCSCs) are responsive to melatonin treatment by way of reducing the viability and the invasiveness of breast cancer mammospheres as well as regulating the expression of OCT4, N-cadherin and vimentin proteins associated with epithelial mesenchymal transition in BCSCs. Carbon irradiation is effective in brain tumor stem cell (BTSC) elimination with relative biologic effectiveness (RBE) in the range of 1.87-3.44. Carbon ion irradiation may be a promising therapeutic modality because it reduces migration and invasion processes in both head and neck squamous cell carcinoma and cancer stem cells in contrast to photon irradiation. Low LET X-ray irradiation may essentially eradicate the non-stem-like tumor cells, consequently the radioresistant cancer stem-like cell population is obviously enriched. In contrast, carbon ion irradiation may eradicate both non-stemlike and stem-like tumor cells at the same time. Carbon ion irradiation is a promising tool to eradicate putative colon cancer stem-like cells. Further investigation to elucidate the mechanisms and molecular pathways involved in cancer stem cells particularly associated with melatonin and carbon ion irradiation certainly is warranted.

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The molecular markers of cancer stem cells in head and neck tumors

Cirillo

2019

Journal Cellular Physiology

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Head and neck cancer (HNC) is the six most common malignancy worldwide leading to more than 350,000 deaths annually. Despite recent advances in treatment modalities for these patients, there has been only a slight improvement of prognosis. As cancer stem cells (CSCs) have been implicated in tumor cell survival, progression, and response to therapy, the identification of this tumor subpopulation would have important therapeutic and prognostic implications. In this structured appraisal of the literature, Embase, PubMed, and Ovid were searched for publications that investigated CSC markers of HNC in humans. The search was conducted under the PRISMA guidelines with clear inclusion and exclusion criteria for articles published in the last two decades.The review process resulted in the identification of some key CSC-associated molecules such as CD44, ALDH1, CD133, Oct3/4, Nanog, and Sox2, although a single common CSC sorting marker could not be found. These biomarkers were identified in a range of HNCs but the most common one was squamous cell carcinoma (SCC), predominantly oral SCC. Patient cohorts were of variable size (3-195 individuals) and the most common technique used for detection was immunohistochemistry. Some of the molecules were associated with poor prognosis and may be able to inform the choice of appropriate treatment for these patients. K E Y W O R D SALDH1, cancer stem cells, CD133, CD44, head and neck cancer, oral squamous cell carcinoma

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CD44^high/ALDH1^high head and neck squamous cell carcinoma cells exhibit mesenchymal characteristics and GSK3β‐dependent cancer stem cell properties

Abstract: Our results show that GSK3β plays a major role in maintenance of stemness of CD44(high) /ALDH1(high) HNSCC cells. Additionally, they indicate a close relationship between CSC and mesenchymal characteristics in HNSCC.

Cited by 29 publications

References 46 publications

Expression and associations of TRAF1, BMI-1, ALDH1, and Lin28B in oral squamous cell carcinoma

Expression and associations of TRAF1, BMI-1, ALDH1, and Lin28B in oral squamous cell carcinoma

The impact of melatonin and carbon ion irradiation on cancer stem cells

The molecular markers of cancer stem cells in head and neck tumors

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CD44high/ALDH1high head and neck squamous cell carcinoma cells exhibit mesenchymal characteristics and GSK3β‐dependent cancer stem cell properties

Abstract: Our results show that GSK3β plays a major role in maintenance of stemness of CD44(high) /ALDH1(high) HNSCC cells. Additionally, they indicate a close relationship between CSC and mesenchymal characteristics in HNSCC.

Cited by 29 publications

References 46 publications

Expression and associations of TRAF1, BMI-1, ALDH1, and Lin28B in oral squamous cell carcinoma

Expression and associations of TRAF1, BMI-1, ALDH1, and Lin28B in oral squamous cell carcinoma

The impact of melatonin and carbon ion irradiation on cancer stem cells

The molecular markers of cancer stem cells in head and neck tumors

Contact Info

Product

Resources

About

CD44^high/ALDH1^high head and neck squamous cell carcinoma cells exhibit mesenchymal characteristics and GSK3β‐dependent cancer stem cell properties