<scp>BSK</scp>s are partially redundant positive regulators of brassinosteroid signaling in <scp>A</scp>rabidopsis

Sreeramulu, Shivakumar; Mostizky, Yana; Sunitha, Sukumaran; Shani, Eilon; Nahum, Hadas; Salomon, Dor; Hayun, Liat Ben; Gruetter, C.; Rauh, Daniel; Ori, Naomi; Sessa, Guido

doi:10.1111/tpj.12175

Cited by 152 publications

(183 citation statements)

References 66 publications

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“…We revealed a possible mechanism with the identification of RLP44 as a modifier of BR signaling, which interacts with the regulatory receptor-like kinase BAK1. Possibly, interaction with RLP44 could favor BAK1-BRI1 interaction or stabilize the interactions in the signaling complex with downstream components, such as receptor-like cytoplasmic kinases (26,27,40).…”

Section: Discussionmentioning

confidence: 99%

A receptor-like protein mediates the response to pectin modification by activating brassinosteroid signaling

Wolf

Does

Ladwig

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

148

187

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The brassinosteroid (BR) signaling module is a central regulator of plant morphogenesis, as indicated by the large number of BRresponsive cell wall-related genes and the severe growth defects of BR mutants. Despite a detailed knowledge of the signaling components, the logic of this auto-/paracrine signaling module in growth control remains poorly understood. Recently, extensive cross-talk with other signaling pathways has been shown, suggesting that the outputs of BR signaling, such as gene-expression changes, are subject to complex control mechanisms. We previously provided evidence for a role of BR signaling in a feedback loop controlling the integrity of the cell wall. Here, we identify the first dedicated component of this feedback loop: a receptor-like protein (RLP44), which is essential for the compensatory triggering of BR signaling upon inhibition of pectin de-methylesterification in the cell wall. RLP44 is required for normal growth and stress responses and connects with the BR signaling pathway, presumably through a direct interaction with the regulatory receptor-like kinase BAK1. These findings corroborate a role for BR in controlling the sensitivity of a feedback signaling module involved in maintaining the physicochemical homeostasis of the cell wall during cell expansion.brassinosteroids | cell wall integrity | pectin

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Section: Discussionmentioning

confidence: 99%

A receptor-like protein mediates the response to pectin modification by activating brassinosteroid signaling

Wolf

Does

Ladwig

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

148

187

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“…The node with the most edges in this subnetwork was a cell wall-related gene, KOR (GRMZM2G099101), and was coexpressed with eight TFs: three bHLH TFs, GRMZM2G074438, GRMZM2G128807, and GRMZM2G158281, the latter showing orthology to Arabidopsis LONESOME HIGHWAY, which is required for establishing and maintaining the normal vascular cell number and pattern in roots (Ohashi-Ito and Bergmann, 2007); two SET domain TFs, GRMZM2G409224 and GRMZM2G318803, the latter being a homolog of Arabidopsis SUVR4; E2F/DP (GRMZM2G462623) and GRF (GRMZM2G099862), both having homologs in Arabidopsis with known roles in leaf development (De Veylder et al, 2002;Kim et al, 2003); and an AGO gene with homology to Arabidopsis AGO4 (GRMZM2G589579). Other genes coexpressed in this subnetwork with know annotations include the cell cycle gene CYCLIN A2 (GRMZM2G017081), involved in vein development in Arabidopsis (Donner and Scarpella, 2013), and BRASSINOSTEROID SIGNALING KINASE2 (GRMZM2G054634), affecting growth through its role in the initial steps of brassinosteroid signal transduction in Arabidopsis (Sreeramulu et al, 2013). Still other genes in this subnetwork were primarily linked to metabolic processes and/or are not well described to date.…”

Section: Toward a Robust Growth Regulatory Networkmentioning

confidence: 99%

Combined Large-Scale Phenotyping and Transcriptomics in Maize Reveals a Robust Growth Regulatory Network

et al. 2016

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Leaves are vital organs for biomass and seed production because of their role in the generation of metabolic energy and organic compounds. A better understanding of the molecular networks underlying leaf development is crucial to sustain global requirements for food and renewable energy. Here, we combined transcriptome profiling of proliferative leaf tissue with indepth phenotyping of the fourth leaf at later stages of development in 197 recombinant inbred lines of two different maize (Zea mays) populations. Previously, correlation analysis in a classical biparental mapping population identified 1,740 genes correlated with at least one of 14 traits. Here, we extended these results with data from a multiparent advanced generation intercross population. As expected, the phenotypic variability was found to be larger in the latter population than in the biparental population, although general conclusions on the correlations among the traits are comparable. Data integration from the two diverse populations allowed us to identify a set of 226 genes that are robustly associated with diverse leaf traits. This set of genes is enriched for transcriptional regulators and genes involved in protein synthesis and cell wall metabolism. In order to investigate the molecular network context of the candidate gene set, we integrated our data with publicly available functional genomics data and identified a growth regulatory network of 185 genes. Our results illustrate the power of combining in-depth phenotyping with transcriptomics in mapping populations to dissect the genetic control of complex traits and present a set of candidate genes for use in biomass improvement

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“…S9B); Therefore, those BZR1-associated proteins that were identified by affinity purification but did not show an interaction in yeast might interact indirectly through other BZR1-interacting proteins, and thus still have a function in BR-regulated processes. The copurification of BSK1 with BZR1 suggests that BR-signaling components exist in a multi-protein complex, possibly involving BIN2 interaction with BRI1, BSU1 (15), and BSK1 (51).…”

Section: Functional Proteomics Is An Effective Approach For Identifyimentioning

confidence: 99%

Identification of BZR1-interacting Proteins as Potential Components of the Brassinosteroid Signaling Pathway in Arabidopsis Through Tandem Affinity Purification

Wang

Shang

Chen

et al. 2013

Molecular & Cellular Proteomics

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Brassinosteroids (BRs) are essential phytohormones for plant growth and development. BRs are perceived by the cell surface receptor kinase BRI1, and downstream signal transduction through multiple components leads to activation of the transcription factors BZR1 and BZR2/BES1. BZR1 activity is highly controlled by BR through reversible phosphorylation, protein degradation, and nucleocytoplasmic shuttling. To further understand the molecular function of BZR1, we performed tandem affinity purification of the BZR1 complex and identified BZR1-associated proteins using mass spectrometry. These BZR1-associated proteins included several known BR signaling components, such as BIN2, BSK1, 14 -3-3, and PP2A, as well as a large number of proteins with previously unknown functions in BR signal transduction, including the kinases MKK5 and MAPK4, histone deacetylase 19, cysteine proteinase inhibitor 6, a DEAD-box RNA helicase, cysteine endopeptidases RD21A and RD21B, calmodulin-binding transcription activator 5, ubiquitin protease 12, cyclophilin 59, and phospholipidbinding protein synaptotagmin A. Their interactions with BZR1 were confirmed by in vivo and in vitro assays. Furthermore, MKK5 was found to phosphorylate BZR1 in vitro. This study demonstrates an effective method for purifying proteins associated with low-abundance transcription factors, and identifies new BZR1-interacting proteins with potentially important roles in BR response. Molecular & Cellular Proteomics 12:

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BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis

Cited by 152 publications

References 66 publications

A receptor-like protein mediates the response to pectin modification by activating brassinosteroid signaling

A receptor-like protein mediates the response to pectin modification by activating brassinosteroid signaling

Combined Large-Scale Phenotyping and Transcriptomics in Maize Reveals a Robust Growth Regulatory Network

Identification of BZR1-interacting Proteins as Potential Components of the Brassinosteroid Signaling Pathway in Arabidopsis Through Tandem Affinity Purification

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