<scp>B</scp>ayesian modelling to estimate the test characteristics of coprology, coproantigen <scp>ELISA</scp> and a novel real‐time <scp>PCR</scp> for the diagnosis of taeniasis

Praet, Nicolas; Verweij, Jaco J.; Mwape, Kabemba E.; Phiri, Isaac K.; Muma, John Bwalya; Zulu, Gideon; Lieshout, Lisette van; Rodríguez‐Hidalgo, Richar; Benítez‐Ortiz, Washington; Dorny, Pierre; Gabriël, Sarah

doi:10.1111/tmi.12089

Cited by 73 publications

(78 citation statements)

References 29 publications

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“…Recently, Bayesian modeling was used to estimate and compare the performances of microscopy, coproantigen ELISA, and multiplex realtime PCR for the detection of Taenia carriers by using stool samples (n ϭ 871) collected in two Zambian communities where the disease is endemic. Specificities were 99.9%, 92.0%, and 99.0% and sensitivities were 82.5%, 84.5%, and 82.7% for microscopy, ELISA, and PCR, respectively (402). In a study comparing LAMP and a conventional multiplex PCR based on the cox1 sequence using 43 known positive fecal samples, LAMP was more sensitive than PCR, 88.4% and 37.2%, respectively (403).…”

Section: Cestodesmentioning

confidence: 99%

Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections

2014

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SUMMARY Over the past few decades, nucleic acid-based methods have been developed for the diagnosis of intestinal parasitic infections. Advantages of nucleic acid-based methods are numerous; typically, these include increased sensitivity and specificity and simpler standardization of diagnostic procedures. DNA samples can also be stored and used for genetic characterization and molecular typing, providing a valuable tool for surveys and surveillance studies. A variety of technologies have been applied, and some specific and general pitfalls and limitations have been identified. This review provides an overview of the multitude of methods that have been reported for the detection of intestinal parasites and offers some guidance in applying these methods in the clinical laboratory and in epidemiological studies.

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Section: Cestodesmentioning

confidence: 99%

Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections

2014

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“…It is particularly significant when atypical forms are found, which are not differentiable from other species under the microscope. There are many studies in which researches use or compare different methods for identification and differentiation of T. solium from other species (4,6,7,15). It seems molecular tools can be considered as the methods of final differentiation of suspicious samples.…”

Section: Discussionmentioning

confidence: 99%

Optimisation and comparison of three PCR procedures for molecular identification of Taenia solium

Karamon

Sroka

Cencek

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was to optimise selected PCR methods for identification of T. solium, and to compare their effectiveness and usefulness. The investigation concerned three PCR methods described earlier: PCR I (specific to oncospherespecific protein Tso31 gene), PCR II (specific to large subunit rRNA gene), and PCR III (cytochrome c oxidases ubunit 1 gene). Each of them needed optimisation in connection with some changes in the procedures. Among the examined procedures, PCR I was found to be the most useful, requiring the least corrections during optimisation -only a higher concentration of polymerase was necessary. Testing an optimised PCR II method showed strong unspecific reactions with E. granulosus and T. saginata. This method was not considered diagnostically useful in distinguishing T. solium. PCR III method yielded products only when annealing temperature was lowered by 2 C. Under such conditions, there were no unspecific reactions with three others Taenidae parasites; however, annealing at a temperature only 1 o C lower generated a distinct unspecific PCR product from T. saginata DNA. Therefore, this method was of limited usefulness. Comparison of the effectiveness of the two selected methods (PCR I and III) in detection of T. solium in successive DNA dilutions showed a large difference between them: in the same DNA sample, PCR I showed positive results in a sample diluted 1:3200, while PCR III failed at dilutions greater than 1:50. The results showed that among the three different methods used in the investigations, the most specific and effective for identification of T. solium was PCR I.

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“…51 In addition, diagnostic tools such as coprology, coproantigen ELISA, and molecular methods (such as polymerase chain reactions) vary widely in their performance in identification of taeniasis cases (with or without accompanying cysticercosis). 52 Coprology methods struggle with sensitivity and species identification; this is especially problematic in SEA where sympatric infections with other Taenia species are common. Coproantigen method is usually only specific to the genus level, although species-specific assays for T. solium have been developed.…”

Section: Diagnostic Methods and Impact On Epidemiologymentioning

confidence: 99%

Taenia solium Taeniasis and Cysticercosis in Southeast Asia

Aung¹,

Spelman²

2016

The American Journal of Tropical Medicine and Hygiene

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Abstract. Human taeniasis/cysticercosis caused by the pork tapeworm Taenia solium has been identified as a potentially eradicable disease by the International Task Force for Disease Eradication of the World Health Organization. In southeast Asia, T. solium taeniasis/cysticercosis is considered one of the major neglected tropical diseases afflicting the region. In the last few decades, a considerable effort has been invested toward establishing the epidemiology and burden of disease in several southeast Asian countries. Moreover, further evidence is emerging as to understanding the dynamics of disease transmission and cultural, political, and socioeconomic factors influencing the success of control and eradication efforts within the region. However, despite major collaborations by several champion groups, advances have been slow and little remains known about the complete epidemiology of taeniasis/cysticercosis and the barriers to programmatic success. This review article aims to address the above issues with a further focus on the challenges to control and eradicate taeniasis/cysticercosis within the southeast Asia region.

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Bayesian modelling to estimate the test characteristics of coprology, coproantigen ELISA and a novel real‐time PCR for the diagnosis of taeniasis

Cited by 73 publications

References 29 publications

Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections

Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections

Optimisation and comparison of three PCR procedures for molecular identification of Taenia solium

Taenia solium Taeniasis and Cysticercosis in Southeast Asia

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