<scp>ASVCP</scp> guidelines: Allowable total error hematology

Nabity, Mary B.; Harr, Kendal E.; Camus, Melinda S.; Flatland, Bente; Vap, Linda M.

doi:10.1111/vcp.12583

Cited by 68 publications

(115 citation statements)

References 28 publications

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“…QCM-based analysis did not allow for the evaluation of HCT or HGB TE obs (since the QCM does not contain RBCs), although the ASVCP TE a guidelines for these measurands are available. 9 Calculation of TE obs for HCT and HGB was based on camelid venous blood data (CV from camelid blood repeatability data and %bias from the method comparison data) and resulted in large TE obs values that likely overestimate the EPOC error. Omitting bias from the TEobs calculation and basing TE obs on CV alone yielded predictably much smaller TE obs results; these did meet TEa recommendations from ASVCP (10% for HCT and 10% for HGB).…”

Section: Discussionmentioning

confidence: 99%

“…Omitting bias from the TEobs calculation and basing TE obs on CV alone yielded predictably much smaller TE obs results; these did meet TEa recommendations from ASVCP (10% for HCT and 10% for HGB). 9 Incidental puncture of the carotid artery has been described as a common occurrence when attempting jugular venipuncture in alpacas and llamas, largely because of their relative lack of neck musculature and the absence of a well-defined jugular groove. 24 results.…”

Section: Discussionmentioning

confidence: 99%

“…TE obs was also calculated using zero for bias. TE obs was compared with ASVCP TE a recommendations for hematology measurands …”

Section: Methodsmentioning

confidence: 99%

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Verification of the Heska Element Point‐of‐Care blood gas instrument for use with venous blood from alpacas and llamas, with determination of reference intervals

Viesselmann

Videla

Flatland

2018

Veterinary Clinical Pathol

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Verification of the Heska Element Point‐of‐Care blood gas instrument for use with venous blood from alpacas and llamas, with determination of reference intervals

Viesselmann

Videla

Flatland

2018

Veterinary Clinical Pathol

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“…The use of TE a as a basis for comparability of results has been previously published by Camus et al, in which an acceptance criterion could be that a sample result is within ± TE a of the reference method result for that sample . The acceptable TE a ranges for the RBC values were based on the American Society for Veterinary Clinical Pathology (ASVCP) guidelines for hematology measurands, which recommend a TE a of 10% for HCT/PCV, RBC, and MCHC, and 7% for MCV …”

Section: Methodsmentioning

confidence: 99%

Evaluation of automated erythrocyte methodology in new world camelids using the ADVIA 2120 hematology analyzer

Viesselmann

Flatland

Stokol

et al. 2019

Veterinary Clinical Pathol

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Background: Accurate erythrocyte measurements with ADVIA hematology analyzers require isovolumetric cell sphering in one reaction and hemolysis in another.However, camelid erythrocytes are resistant to sphering and osmotic lysis, and no published evaluation of ADVIA methods for camelids exists. Objectives:The objectives were to demonstrate whether camelid erythrocytes sphere in the ADVIA red blood cell/platelet (RBC/PLT) reagent and lyse in the ADVIA cyanide HGB reagent, and to determine optimal ADVIA settings for camelids.Methods: Camelid and canine blood were diluted to 1:625 in RBC/PLT reagent and evaluated microscopically for erythrocyte sphering. A camelid sample was incubated with the hemoglobin (HGB) reagent at varying dilutions to evaluate hemolysis.The RBC, hematocrit (HCT), mean cell volume (MCV), and mean corpuscular hemoglobin concentration (MCHC) using three ADVIA species settings (equine, bovine, and caprine) were compared to their respective reference methods: Z2 Coulter impedance counter, packed cell volume, calculated MCV (PCV × 10/Coulter RBC), and calculated MCHC (HGB × 100/PCV). Reference MCV was also compared to MCV calculated using the ADVIA equine RBC count. Comparisons were assessed using Passing-Bablok regression and Bland-Altman difference plots.Results: Camelid erythrocytes did not sphere in the RBC/PLT reagent, but did lyse in the HGB reagent. The ADVIA equine setting RBC count was acceptably close to the Coulter count. Hematocrit, MCV, and MCHC from all settings were significantly different from the reference methods. Mean cell volumes calculated using the equine setting RBC counts were acceptably close to the reference MCV. Conclusions:Camelid ADVIA erythrogram results should be reported as follows: RBC counts and HGB concentrations using the equine setting, spun PCVs, MCVs calculated using the PCV and equine setting RBC, and MCHCs calculated using the PCV and equine setting HGB. K E Y W O R D Sallowable total error, alpaca, bias, llama, red blood cells | INTRODUC TI ONCamelid erythrocytes are small, flat, and elliptical, with rigid cell membranes. 1-3 Small and flat erythrocytes have a higher surface area to volume ratio than larger and thicker erythrocytes. This high surface to volume ratio allows for more efficient gas exchange at the cell surface, which can be important for New World camelids in the high altitude, decreased oxygen environment of the Andes

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“…The mean and standard deviation (SD) of the 3 measurements of each horse were calculated to determine the individual horse CV, dividing the SD by the mean. 7,14 Overall CV was calculated by averaging the individual CVs.…”

Section: Research-article2019mentioning

confidence: 99%

Assessment of the CoaguChek-XS portable prothrombin time point-of-care analyzer for horses

Berlin¹,

Kelmer²,

Segev³

et al. 2019

J VET Diagn Invest

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In horses, gastrointestinal (GI) disorders are the main cause of disseminated intravascular coagulation (DIC), a potentially life-threatening condition. Large colon volvulus, colitis, and strangulating small intestinal lesions are the most common GI conditions that cause DIC in horses, 4,12 but DIC is also reported in septicemia, snakebites, rhabdomyolysis, and diffuse clostridial myonecrosis. 11,13,16,19,21,22 Significantly prolonged PT was reported in 58% of horses with acute GI crisis-associated DIC, 12 and was significantly associated with strangulating GI lesions. 3,4 Early diagnosis of subclinical DIC is crucial, and depends on laboratory testing of hemostasis. 3 Prothrombin time (PT) is commonly measured in horses to assess the status of hemostasis, specifically the extrinsic and common pathways. 21 Monitoring changes in PT over time is of diagnostic and prognostic value, as well as a clinical tool to help guide treatment. 3,4 Equine medicine is often practiced at a distance from reference laboratories. Delays in receiving hemostasis test results may be detrimental in treating critically ill horses. A reliable, cost-effective, point-of-care (POC) test to assess hemostasis, such as PT measurement, would be useful under field conditions. The CoaguChek-XS (Roche Diagnostics, Mannheim, Germany) is a small, portable POC PT analyzer, commonly used in human medicine for home monitoring of warfarin therapy, that is suitable for both venous and capillary blood samples, providing results within minutes. 17,20 Studies have assessed the reliability, precision, and suitability of the CoaguChek-XS

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ASVCP guidelines: Allowable total error hematology

Cited by 68 publications

References 28 publications

Verification of the Heska Element Point‐of‐Care blood gas instrument for use with venous blood from alpacas and llamas, with determination of reference intervals

Verification of the Heska Element Point‐of‐Care blood gas instrument for use with venous blood from alpacas and llamas, with determination of reference intervals

Evaluation of automated erythrocyte methodology in new world camelids using the ADVIA 2120 hematology analyzer

Assessment of the CoaguChek-XS portable prothrombin time point-of-care analyzer for horses

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