<scp>A</scp>ccelerated skin wound healing using electrospun nanofibrous mats blended with mussel adhesive protein and polycaprolactone

Kim, Bum Jin; Cheong, Hogyun; Choi, Eunhwa; Yun, So Hee; Choi, Bong‐Hyuk; Park, Ki-Soo; Kim, Ick Soo; Park, Dae-Hwan; Joon, Hyung

doi:10.1002/jbm.a.35903

Cited by 58 publications

(22 citation statements)

References 38 publications

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“…When used as an external dressing material, nanofibers could be used as a release vehicle for the chemical antiseptic chlorohexidine [12]. Multifunctional electrospun nanofibrous mats of mussel adhesive protein and PCL accelerated regeneration in a rat skin wound-healing model [13].…”

Section: Discussionmentioning

confidence: 99%

Radially patterned polycaprolactone nanofibers as an active wound dressing agent

Shin

Kim²,

Yang

et al. 2019

Arch Plast Surg

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BackgroundThe objectives of this study were to design polycaprolactone nanofibers with a radial pattern using a modified electrospinning method and to evaluate the effect of radial nanofiber deposition on mechanical and biological properties compared to non-patterned samples.MethodsRadially patterned polycaprolactone nanofibers were prepared with a modified electrospinning method and compared with randomly deposited nanofibers. The surface morphology of samples was observed under scanning electron microscopy (SEM). The tensile properties of nanofibrous mats were measured using a tabletop uniaxial testing machine. Fluorescence-stained human bone marrow stem cells were placed along the perimeter of the radially patterned and randomly deposited. Their migration toward the center was observed on days 1, 4, and 7, and quantitatively measured using ImageJ software.ResultsOverall, there were no statistically significant differences in mechanical properties between the two types of polycaprolactone nanofibrous mats. SEM images of the obtained samples suggested that the directionality of the nanofibers was toward the central area, regardless of where the nanofibers were located throughout the entire sample. Florescence images showed stronger fluorescence inside the circle in radially aligned nanofibers, with significant differences on days 4 and 7, indicating that migration was quicker along radially aligned nanofibers than along randomly deposited nanofibers.ConclusionsIn this study, we successfully used modified electrospinning to fabricate radially aligned nanofibers with similar mechanical properties to those of conventional randomly aligned nanofibers. In addition, we observed faster migration along radially aligned nanofibers than along randomly deposited nanofibers. Collectively, the radially aligned nanofibers may have the potential for tissue regeneration in combination with stem cells.

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Section: Discussionmentioning

confidence: 99%

Radially patterned polycaprolactone nanofibers as an active wound dressing agent

Shin

Kim²,

Yang

et al. 2019

Arch Plast Surg

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“…Electrospinning is a versatile technique and has been used for many tissue engineering applications, including (1) skin tissue, using poly(lactic-co-glycolic acid) (PLGA) scaffolds (Ru et al 2015 ), (2) bone tissue using polycaprolactone (PCL), PLLA, silk, and collagen (Khajavi et al 2016 ), (3) corneal tissue, using poly- l -lactic acid (PLLA) PCL, PLGA, gelatine, silk, and collagen (Kong and Mi 2016 ), (4) cardiac tissue using PLGA scaffolds (P Prabhakaran et al 2011 ), (5) drug delivery (Mirjalili and Zohoori 2016 ) (6) wound healing (Askari et al 2016 )and (7) detection of metal ions (Kim et al 2017 ).…”

Section: Scaffold Fabrication Techniquesmentioning

confidence: 99%

New generation of bioreactors that advance extracellular matrix modelling and tissue engineering

et al. 2018

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Bioreactors hold a lot of promise for tissue engineering and regenerative medicine applications. They have multiple uses including cell cultivation for therapeutic production and for in vitro organ modelling to provide a more physiologically relevant environment for cultures compared to conventional static conditions. Bioreactors are often used in combination with scaffolds as the nutrient flow can enhance oxygen and diffusion throughout the 3D constructs to prevent the formation of necrotic cores. A variety of scaffolds have been fabricated to achieve a structural architecture that mimic native extracellular matrix. Future developments of in vitro models will incorporate the ability to non-invasively monitor the cellular microenvironment to enhance the understanding of in vitro conditions. This review details current advancements in bioreactor and scaffold systems and provides insight on how in vitro models can be augmented for future biomedical applications.

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“…Electrohydrodynamic atomisation (EHDA), more specifically electrospinning was utilised in this instance to engineer fibrous coatings for contact lens surfaces. The on-demand, cost effective process has already shown its potential in an array of applications [7] including wound management [8][9][10][11][12][13], drug delivery [14][15][16][17][18][19][20][21][22] bioengineering [23][24][25][26] and theranostics [27]. Here, electrospun fibrous coatings were engineered to assess the ex vivo release and permeation of TM through freshly excised cornea as an extension of previous work [18].…”

Section: Introductionmentioning

confidence: 99%

Assessing the ex vivo permeation behaviour of functionalised contact lens coatings engineered using an electrohydrodynamic technique

et al. 2018

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In vitro testing alone is no longer considered sufficient evidence presented solely with respect to drug release and permeation testing. These studies are thought to be more reliable and representative when using tissue or animal models; as opposed to synthetic membranes. The release of anti-glaucoma drug timolol maleate from electrically atomised coatings was assessed here using freshly excised bovine corneal tissue. Electrohydrodynamic processing was utilised to engineer functionalised fibrous polyvinylpyrrolidone-Poly (N-isopropylacrylamide) coatings on the outer side of commercial silicone contact lenses. Benzalkonium chloride, ethylenediaminetetraacetic acid, Brij ® 78 and borneol were employed as permeation enhancers to see their effect on ex vivo permeation of timolol maleate through the cornea. Formulations containing permeation enhancers showed a vast improvement with respect to cumulative amount of drug permeating through the cornea as shown by a six fold decrease in lag time compared to enhancer-free formulations. Most drug delivery systems require the drug to pass or permeate through a tissue or biological membrane. This study has shown that to fully appreciate and understand how a novel drug delivery system will behave not only within the device but with the external environment or tissue, it is imperative to have in vitro and ex vivo data in conjunction.

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Accelerated skin wound healing using electrospun nanofibrous mats blended with mussel adhesive protein and polycaprolactone

Cited by 58 publications

References 38 publications

Radially patterned polycaprolactone nanofibers as an active wound dressing agent

Radially patterned polycaprolactone nanofibers as an active wound dressing agent

New generation of bioreactors that advance extracellular matrix modelling and tissue engineering

Assessing the ex vivo permeation behaviour of functionalised contact lens coatings engineered using an electrohydrodynamic technique

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