<scp>3D</scp> bioprinting of <scp>photo‐crosslinkable</scp> silk methacrylate (<scp>SilMA</scp>)‐polyethylene glycol diacrylate (<scp>PEGDA</scp>) bioink for cartilage tissue engineering

Bandyopadhyay, Ashutosh; Mandal, Biman B.; Bhardwaj, Nandana

doi:10.1002/jbm.a.37336

Cited by 55 publications

(24 citation statements)

References 63 publications

(153 reference statements)

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“…PEGDA and PEGMA have been mixed with other polymers, such as alginate, to increase viscosity. In particular, PEGDA is a promising biomaterial for CTE since it can be injectable less invasively and can differentiate the MSCs into chondrocyte-like cells in in vitro conditions . Skaalure et al developed a new biodegradable cartilage-specific PEG-based hydrogel and encapsulated bovine chondrocytes for cartilage tissue engineering .…”

Section: Discussionmentioning

confidence: 99%

“…In particular, PEGDA is a promising biomaterial for CTE since it can be injectable less invasively and can differentiate the MSCs into chondrocytelike cells in in vitro conditions. 58 Skaalure et al developed a new biodegradable cartilage-specific PEG-based hydrogel and encapsulated bovine chondrocytes for cartilage tissue engineering. 59 De France et al have designed an in situ gelling nanocomposite hydrogel based on PEGMA and rigid rodlike cellulose nanocrystals.…”

Section: Discussionmentioning

confidence: 99%

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3D Bioprintable Hypoxia-Mimicking PEG-Based Nano Bioink for Cartilage Tissue Engineering

Ravi

Chokkakula

Giri

et al. 2023

ACS Appl. Mater. Interfaces

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As hypoxia plays a significant role in the formation and maintenance of cartilage tissue, aiming to develop native hypoxia-mimicking tissue engineering scaffolds is an efficient method to treat articular cartilage (AC) defects. Cobalt (Co) is documented for its hypoxic-inducing effects in vitro by stabilizing the hypoxia-inducible factor-1α (HIF-1α), a chief regulator of stem cell fate. Considering this, we developed a novel three-dimensional (3D) bioprintable hypoxia-mimicking nano bioink wherein cobalt nanowires (Co NWs) were incorporated into the poly(ethylene glycol) diacrylate (PEGDA) hydrogel system as a hypoxia-inducing agent and encapsulated with umbilical cord-derived mesenchymal stem cells (UMSCs). In the current study, we investigated the impact of Co NWs on the chondrogenic differentiation of UMSCs in the PEGDA hydrogel system. Herein, the hypoxia-mimicking nano bioink (PEGDA+Co NW) was rheologically optimized to bioprint geometrically stable cartilaginous constructs. The bioprinted 3D constructs were evaluated for their physicochemical characterization, swelling-degradation behavior, mechanical properties, cell proliferation, and the expression of chondrogenic markers by histological, immunofluorescence, and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) methods. The results disclosed that, compared to the control (PEGDA) group, the hypoxia-mimicking nano bioink (PEGDA+Co NW) group outperformed in print fidelity and mechanical properties. Furthermore, live/dead staining, double-stranded DNA (dsDNA) content, and glycosaminoglycans (GAGs) content demonstrated that adding low amounts of Co NWs (<20 ppm) into PEGDA hydrogel system supported UMSC adhesion, proliferation, and differentiation. Histological and immunofluorescence staining of the PEGDA+Co NW bioprinted structures revealed the production of type 2 collagen (COL2) and sulfated GAGs, rendering it a feasible option for cartilage repair. It was further corroborated by a significant upregulation of the hypoxia-mediated chondrogenic and downregulation of the hypertrophic/osteogenic marker expression. In conclusion, the hypoxia-mimicking hydrogel system, including PEGDA and Co 2+ ions, synergistically directs the UMSCs toward the chondrocyte lineage without using expensive growth factors and provides an alternative strategy for translational applications in the cartilage tissue engineering field.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

3D Bioprintable Hypoxia-Mimicking PEG-Based Nano Bioink for Cartilage Tissue Engineering

Ravi

Chokkakula

Giri

et al. 2023

ACS Appl. Mater. Interfaces

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“…In addition to printing technologies, the formulation of bio-inks, usually in the combination of supportive biomaterials and specific cell types, is critical for the success of bioprinting. The biomaterials used in bioprinting can be roughly divided into two subtypes: (1) natural biomaterials, such as alginate [ 14 , 35 , 36 , 37 ], agarose [ 35 , 38 ], collagen [ 39 , 40 ], and nanocellulose [ 41 ]; and (2) synthetic biomaterials, such as polyethylene glycol diacrylate (PEDGA) [ 42 ], and Pluoronic ® [ 43 , 44 ]. Each material has unique mechanical (e.g., printability) and biological properties (e.g., the ability to support long-term cell adhesion and growth).…”

Section: Bioprinting: Methods and Materialsmentioning

confidence: 99%

3D Bioprinting for Next-Generation Personalized Medicine

Lam

Zhu

et al. 2023

IJMS

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In the past decade, immense progress has been made in advancing personalized medicine to effectively address patient-specific disease complexities in order to develop individualized treatment strategies. In particular, the emergence of 3D bioprinting for in vitro models of tissue and organ engineering presents novel opportunities to improve personalized medicine. However, the existing bioprinted constructs are not yet able to fulfill the ultimate goal: an anatomically realistic organ with mature biological functions. Current bioprinting approaches have technical challenges in terms of precise cell deposition, effective differentiation, proper vascularization, and innervation. This review introduces the principles and realizations of bioprinting with a strong focus on the predominant techniques, including extrusion printing and digital light processing (DLP). We further discussed the applications of bioprinted constructs, including the engraftment of stem cells as personalized implants for regenerative medicine and in vitro high-throughput drug development models for drug discovery. While no one-size-fits-all approach to bioprinting has emerged, the rapid progress and promising results of preliminary studies have demonstrated that bioprinting could serve as an empowering technology to resolve critical challenges in personalized medicine.

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“…In recent years various types of hydrogels based on natural and synthetic polymers were employing multiple different cross-linking mechanisms. − It has been shown that the appropriate choice of chemical cross-linking approach leads to the generation of hydrogel networks with properties tailored to the desired clinical or tissue engineering application. − Among all types of hydrogels, peptide- and protein-based hydrogels have been widely studied as biocompatible matrices for numerous biomedical applications. ,− These molecules typically form safe and effective biomaterials due to their ability to form stable networks under mild conditions, their excellent intrinsic biocompatibility, as well as tunable biochemical and biophysical properties. ,,,− Their physical and biochemical properties depend on the composition, the polymerization method, and the type and degree of cross-linking . The utilization of cross-linking approaches has led to the development of many applications for these hydrogels in biomedical science, especially in designing injectable cell and drug cargo vehicles. ,, Moreover, they have become an integral part of 3D culture research to investigate the cellular proliferation and migration as well as interactions between encapsulated cells (cell–cell) and cells with the matrix (cell–material). , Since they mimic the structure and characteristics of extracellular matrix (ECM), they appear as ideal mimicking biomaterials for many biomedical applications. ,,,, …”

Section: Introductionmentioning

confidence: 99%

“…This component is widely utilized in the preparation of photo-cross-linkable hydrogels that are cured using light. In addition, PEGDA hydrogels are bioinert, nontoxic, nonimmunogenic, possess tunable physical and chemical properties, and have been shown to be injectable. , Bandyopadhyay et al have developed a bioink made of composite bioink in order to promote cartilage regeneration (Figure B) . SFMA, PEGDA, and gelatin have been used as the main components of their bioink.…”

Section: Chemical Modification Of the Sf For Introducing The Methacry...mentioning

confidence: 99%

Versatile Potential of Photo-Cross-Linkable Silk Fibroin: Roadmap from Chemical Processing Toward Regenerative Medicine and Biofabrication Applications

Amirian,

Wychowaniec,

Amel Zendehdel

et al. 2023

Biomacromolecules

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Over the past two decades, hydrogels have come to the forefront of tissue engineering and regenerative medicine due to their biocompatibility, tunable degradation and low immunogenicity. Due to their porosity and polymeric network built up, it is possible to incorporate inside drugs, bioactive molecules, or other biochemically active monomers. Among biopolymers used for the fabrication of functional hydrogels, silk fibroin (SF) has received considerable research attention owing to its known biocompatibility and tunable range of mechanical properties. However, its relatively simple structure limits the potential usability. One of the emerging strategies is a chemical functionalization of SF, allowing for the introduction of methacrylate groups. This allows the versatile processing capability, including photocross-linking, which makes SF a useful polymer as a bioink for 3D printing. The methacrylation reaction has been done using numerous monomers such as methacrylic anhydride (MA), 2-isocyanatoethyl methacrylate (IEM), or glycidyl methacrylate (GMA). In this Review, we summarize the chemical functionalization strategies of SF materials and their resulting physicochemical properties. More specifically, a brief explanation of the different functionalization methods, the cross-linking principles, possibilities, and limitations of methacrylate compound functionalization are provided. In addition, we describe types of functional SF hydrogels and link their design principles to the performance in applications in the broad fields of biofabrication, tissue engineering, and regenerative medicine. We anticipate that the provided guidelines will contribute to the future development of SF hydrogels and their composites by providing the rational design of new mechanisms linked to the successful realization of targeted biomedical application.

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3D bioprinting of photo‐crosslinkable silk methacrylate (SilMA)‐polyethylene glycol diacrylate (PEGDA) bioink for cartilage tissue engineering

Cited by 55 publications

References 63 publications

3D Bioprintable Hypoxia-Mimicking PEG-Based Nano Bioink for Cartilage Tissue Engineering

3D Bioprintable Hypoxia-Mimicking PEG-Based Nano Bioink for Cartilage Tissue Engineering

3D Bioprinting for Next-Generation Personalized Medicine

Versatile Potential of Photo-Cross-Linkable Silk Fibroin: Roadmap from Chemical Processing Toward Regenerative Medicine and Biofabrication Applications

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