Scotopic electroretinography in fishing cat (<i>Prionailurus viverrinus</i>) and leopard cat (<i>Prionailurus bengalensis</i>)

Sussadee, Metita; Vorawattanatham, Narathip; Pinyopummin, Anuchai; Phavaphutanon, Janjira; Thayananuphat, Aree

doi:10.1111/vop.12414

Cited by 4 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Before inducing dark adaptation, an illuminance meter (TES‐1339; TES Electrical Electronic Crop) was used to measure background luminance under the shading cloth to confirm that the dark adaptation background luminance is 0 lux. There has been no report on the optimal dark adaptation time for ERG tests in giant pandas, but the recommended dark adaptation time in dogs, cats, and horses is 20 minutes, 6‐8 so we adopted a dark adaptation time of 20 minutes for giant pandas in this study.…”

Section: Methodsmentioning

confidence: 99%

Electroretinography (ERG) in the wild giant panda (Ailuropoda melanoleuca)

Jin

Wei

et al. 2021

Veterinary Ophthalmology

View full text Add to dashboard Cite

Purpose Using a quick electroretinography (ERG) protocol for rapid assessment of the retinal function of wild giant pandas (Ailuropoda melanoleuca) performed in field conditions to demonstrate the range of ERG recordings in giant pandas of unknown retinal status. Animals studied Nine free range giant pandas. Procedure All the giant pandas were anesthetized using an intramuscular dexMTZ injection, which is a combination of dexmedetomidine and tiletamine‐zolazepam. After 20 mins of dark adaptation, scotopic ERGs were obtained by using three flash intensities: −25 dB (0.0087 cd·s/m2), 0 dB (2.75 cd·s/m2), and +5 dB (8.7 cd·s/m2). Next, photopic ERGs were acquired using a single flash protocol with a flash intensity of 3.0 cd·s/m2 after 10 minutes of light adaptation. Results In scotopic ERG at 0.0087 cd·s/m2, mean b‐wave amplitude and peak time were 82.26 µV (SD ± 16.65 and 95% CI 68.33‐96.18) and 66.97 ms (SD ± 10.86 and 95% CI 57.90‐76.05), respectively. This flash intensity was below a‐wave threshold and resulted in b waves with greater peak times compared to those with higher intensities. At 2.75 cd·s/m2, the mean a‐wave amplitude and peak time were 53.95 µV (SD ± 11.63 and 95% CI 44.23‐63.67) and 16.13 ms (SD ± 2.62 and 95% CI 13.94‐18.31), and mean b‐wave amplitude and peak time were 119.57 µV (SD ± 15.54 and 95% CI 106.57‐132.56) and 32.00 ms (SD ± 6.47 and 95% CI 26.59‐37.41). At 8.7 cd·s/m2, the mean a‐wave amplitude and peak time were 58.85 µV (SD ± 14.90 and 95% CI 46.39‐71.31) and 15.59 ms (SD ± 2.63 and 95% CI 13.40‐17.79), and the mean b‐wave amplitude and peak time were 132.97 µV (SD ± 22.11 and 95% CI 114.48‐151.46) and 32.66 ms (SD ± 6.87 and 95% CI 26.91‐38.40). In photopic ERG at 2.75 cd·s/m2, the mean a‐wave amplitude and peak time were 62.08 µV (SD ± 16.61 and 95% CI 48.19‐75.97) and 16.28 ms (SD ± 0.90 and 95% CI 15.53‐17.03), and the mean b‐wave amplitude and peak time were 214.93 µV (SD ± 70.41 and 95% CI 156.07‐273.80) and 33.09 ms (SD ± 1.27 and 95% CI 32.03‐34.15). Conclusion Using a portable ERG system with a brief ERG protocol to perform electroretinographies in wild giant pandas is a practical, useful, and reliable method for the rapid assessment of their retinal function.

show abstract

Section: Methodsmentioning

confidence: 99%

Electroretinography (ERG) in the wild giant panda (Ailuropoda melanoleuca)

Jin

Wei

et al. 2021

Veterinary Ophthalmology

View full text Add to dashboard Cite

show abstract

“…They inhabit Eurasia, the Americas, and potentially northernmost Africa (Duarte and Gonzalez, 2010). In Brazil, many species of neotropical deer have been exhibiting sharp population decline, among other serious problems, due to habitat loss, disease, and hunting (Tomas et al, 1997). Knowledge of the morpho-functionality of deer body structures is a critical part of their conservation research programs.…”

Section: Introductionmentioning

confidence: 99%

Electroretinography in eight species of neotropical deer

Crivelaro

Thiesen

Aldrovani

et al. 2018

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

The aim of this study was to establish normal baseline ERG values of 23 anesthetized deer belonging to 8 neotropical species (Mazama americana, Mazama nemorivaga, Mazama gouazoubira, Mazama nana, Mazama bororo, Ozotocerus bezoarticus, Odocoileus virginianus and Blastocerus dichotomus). Only right eyes were studied. Chemical restraint was performed using xylazine associated with ketamine, IM, for M. americana, M. gouazoubira, M. nemorivaga, M. nana, M. bororo, O. bezoarticus and O. virginianus. A combination of tiletamine/zolazepam diluted in xylazine 2% was used for B. dichotomus individuals. After 20min of dark adaptation, electroretinograms were obtained using a handheld electroretinography (ERG) machine using the QuickRetCheck Protocol at three different light intensities: 0.01cd.s/m2, 3cd.s/m2, and 10cd.s/m2. After light adaptation, photopic phase was recorded. A-wave amplitude recorded during pattern mixed rod/cone response in M. americana was significantly lower when compared to B. dichotomus. No other differences were observed between the species studied. ERG in Neotropical deer is applicable using a portable ERG system and did not show differences among species in relation to the retinal response at different light intensities. Therefore, the lifestyle of the species is more dictated by the selection pressure of the environment than by physiological factors.

show abstract