Scorpion stings in Mexico and their treatment

Calderón-Aranda, Emma S.; Dehesa-Dávila, Manuel; Chávez-Haro, Alfredo Luis; Possani, Lourival D.

doi:10.1016/0041-0101(96)83688-9

Cited by 11 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antivenom currently used in Mexico consists of bivalent F(ab 0 ) 2 fragments obtained from hyper-immunized horses with a water extract from venomous glands of Centruroides scorpions. 2 The monoclonal antibody BCF2 neutralizes the deadly effects of Cn2 toxin, which is present in the venom of one of the most dangerous species of Mexican scorpions (Centruroides noxius Hoffman). 3 Cn2 is one of the most abundant (6.8% of total venom), and toxic (LD 50 Z0.25 mg/20 g mouse) peptides of this venom.…”

Section: Introductionmentioning

confidence: 99%

Directed Evolution, Phage Display and Combination of Evolved Mutants: A Strategy to Recover the Neutralization Properties of the scFv Version of BCF2 a Neutralizing Monoclonal Antibody Specific to Scorpion Toxin Cn2

Juárez-González¹,

Riaño-Umbarila²,

Quintero-Hernández³

et al. 2005

Journal of Molecular Biology

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Directed Evolution, Phage Display and Combination of Evolved Mutants: A Strategy to Recover the Neutralization Properties of the scFv Version of BCF2 a Neutralizing Monoclonal Antibody Specific to Scorpion Toxin Cn2

Juárez-González¹,

Riaño-Umbarila²,

Quintero-Hernández³

et al. 2005

Journal of Molecular Biology

View full text Add to dashboard Cite

“…The state of Morelos ranks among the first places of scorpion sting incidence rates in Mexico, with an average of 1778 cases per 100 000 inhabitants. This makes scorpion stings the fifth mortality cause in the state (Secretaría de Salud, 2002, 2003, 2004, 2005, 2006, 2007, 2008a, 2009, 2010, probably due to the presence of C. limpidus limpidus in 60% of the region in Morelos (Calderon-Aranda et al, 1996;Santibañez-López et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…The most relevant abiotic factors for scorpion activity are pluvial precipitation and surface ambient temperature. The combination of temperature decrease and precipitation result in a reduction of scorpion surface activity (Polis, 1980;Bradley, 1988;Calderon-Aranda et al, 1996;Araujo et al, 2010;Nime et al, 2013). However, precipitation alone can stimulate scorpion surface activity (Araujo et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Influence of increasing temperature on the scorpion sting incidence by climatic regions

Hurtado-Díaz

Guzmán-Ontiveros²,

Arias-Medellín

et al. 2017

Intl Journal of Climatology

View full text Add to dashboard Cite

Scorpion stings are a major public health concern representing a frequent cause of intoxication in many countries. The present study aims to examine the impact of climate variability in different climatic regions of the state of Morelos in Mexico on the incidence of scorpion stings. An ecological study was conducted using a retrospective time‐series analysis. Weekly reported scorpion sting cases from the Unique System of Information for Epidemiological Surveillance (SUIVE) and daily temperature and precipitation from the National Meteorological Service were obtained from 1999 to 2007. The geographical region of study was divided according three climatic conditions into Hot region (HR), Warm region (WR) and Cold region (CR). Generalized Linear Models were used to estimate the percent change [95% confidence intervals (CI)] of scorpion sting cases associated to an increase of 1 °C of temperature and 1 cm of precipitation in each climatic region. During the period of study there were 281 076 scorpion sting cases reported in the State of Morelos, Mexico. Positive correlations among temperature and scorpion sting cases were found (R = 0.59 and 0.70 in the HR and WR, respectively). Regions with the hottest temperatures had the greatest effect, showing a 9.8% (CI 95%: 8.30–11.30) increase in scorpion sting cases per 1 °C increased in temperature. Increase in minimum and maximum temperatures have a delayed effect on scorpion stings cases and these may vary by climatic region.

show abstract

“…In many parts of the world scorpion stings represent an important public health problem. In Mexico, several species of the genus Centruroides are medically important [1,2]. The molecules responsible for the toxicity of the scorpion venom are polypeptides of 4–8 kDa (reviewed in [3]).…”

mentioning

confidence: 99%

Mapping of an epitope recognized by a neutralizing monoclonal antibody specific to toxin Cn2 from the scorpion Centruroides noxius, using discontinuous synthetic peptides

Calderón-Aranda

Selisko

York

et al. 1999

European Journal of Biochemistry

View full text Add to dashboard Cite

The Na + -channel-affecting toxin Cn2 represents the major and one of the most toxic components of the venom of the Mexican scorpion Centruroides noxius Hoffmann. A monoclonal antibody BCF2 raised against Cn2 has been shown previously to be able to neutralize the toxic effect of Cn2 and of the whole venom of C. noxius. In the present study the epitope was mapped to a surface region comprising the N-and C-terminal segments of Cn2, using continuous and discontinuous synthetic peptides, designed on the basis of the sequence and a threedimensional model of Cn2. The study of peptides of varying length resulted in the identification of segments 5±14 and 56±65 containing residues essential for recognition by BCF2. The peptide (abbreviated SP7) with the highest affinity to BCF2 (IC 50 = 5.1 mm) was a synthetic heterodimer comprising the amino acid sequence from position 3±15 (amidated) of Cn2, bridged by disulfide to peptide from position 54±66, acetylated and amidated. Similar affinity was found with peptide SP1 [heterodimer comprising residues 1±14 (amidated) of Cn2, bridged with synthetic peptide 52±66 (acetylated)]. SP1 and SP7 were used to induce anti-peptide antibodies in mouse and rabbit. Both peptides were highly immunogenic. The sera obtained were able to recognize Cn2 and to neutralize Cn2 in vitro. The most efficient protection (8.3 mg Cn2 neutralized per mL of serum) was induced by rabbit anti-SP1 serum.

show abstract

Scorpion stings in Mexico and their treatment

Cited by 11 publications

References 0 publications

Directed Evolution, Phage Display and Combination of Evolved Mutants: A Strategy to Recover the Neutralization Properties of the scFv Version of BCF2 a Neutralizing Monoclonal Antibody Specific to Scorpion Toxin Cn2

Directed Evolution, Phage Display and Combination of Evolved Mutants: A Strategy to Recover the Neutralization Properties of the scFv Version of BCF2 a Neutralizing Monoclonal Antibody Specific to Scorpion Toxin Cn2

Influence of increasing temperature on the scorpion sting incidence by climatic regions

Mapping of an epitope recognized by a neutralizing monoclonal antibody specific to toxin Cn2 from the scorpion Centruroides noxius, using discontinuous synthetic peptides

Contact Info

Product

Resources

About