Scorpine, an anti‐malaria and anti‐bacterial agent purified from scorpion venom

Condé, Renaud; Zamudio, Fernando Z.; Rodrı́guez, Mario H.; Possani, Lourival D.

doi:10.1016/s0014-5793(00)01384-3

Cited by 236 publications

(178 citation statements)

References 14 publications

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“…Scorpine decreased in a dose-dependent manner the fecundation of P. berghei parasites (measured as number of rosettes) and the formation of ookinetes (Conde et al 2000). The inhibition of ookinetes formation in P. berghei was confirmed by Carballar-Lejarazù and colleagues, who also demonstrated the inhibition of asexual P. falciparum parasites in vitro (Carballar-Lejarazú et al 2008).…”

Section: Scorpinementioning

confidence: 74%

See 1 more Smart Citation

Beyond Lysozyme: Antimicrobial Peptides Against Malaria

D’Alessandro

Tullio

Giribaldi

2014

Human and Mosquito Lysozymes

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Antimicrobial peptides (AMPs) are short amino acidic sequences with less than 100 residues. They are the components of the innate immune system not only in humans but also in plants, insects, and primitive multicellular organisms. Their role is to counteract the microorganisms, which could be potentially pathogenic for the host. AMPs active against viruses, bacteria, fungi, and parasites have been described. Among the antiparasitic AMPs reported so far, some peptides affect Plasmodium development in different phases of the biological cycle, from asexual blood stages to sexual stages in the mosquito, where AMPs can block ookinetes viability or oocyst formation. AMPs with antimalarial activity derive from different organisms, especially insects, as well as amphibians. In malaria research, AMPs have been mainly proposed for the engineering of mosquitoes or parasites to reduce or interrupt the malaria parasite transmission. In this chapter, the different classes of antimalarial AMPs (defensins, cecropins, dermaseptins) or single peptides (scorpine, melittin, gambicin) are described. 91

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Section: Scorpinementioning

confidence: 74%

“…It was tested for the first time against Plasmodium due to its similarity, in the peptide sequence, to cecropins and defensins, already known for their antimalarial activity (Conde et al 2000).…”

Section: Scorpinementioning

confidence: 99%

Beyond Lysozyme: Antimicrobial Peptides Against Malaria

D’Alessandro

Tullio

Giribaldi

2014

Human and Mosquito Lysozymes

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“…Just like the AMPs found in mammal skin, the AMPs found in scorpion venom are also important and diverse. Since the first two antimicrobial peptides hadrurin (47) and scorpine (11) were isolated from the scorpion Hadrurus aztecus and the scorpion Pandinus imperator, respectively, some AMPs were isolated and identified from the venoms of different scorpion species. The venom gland cDNA library of the scorpion Chaerilus tricostatus was constructed first.…”

Section: Discussionmentioning

confidence: 99%

“…It has long been widely believed that scorpion venom is a mixture of natural bioactive substances (35). At present, some antimicrobial peptides have been found in scorpion venom and hemolymph (8,20), such as hadrurin (47), scorpine (11), opistoporins, parabutoporin (33), IsCTs (14), pandinins (12), mucroporin (13), imcroporin (53), and StCT1 (51), indicating that scorpion venom is also a rich resource of crude antimicrobial peptides.…”

mentioning

confidence: 99%

Ctriporin, a New Anti-Methicillin-Resistant Staphylococcus aureus Peptide from the Venom of the Scorpion Chaerilus tricostatus

Fan

Cao

et al. 2011

Antimicrob Agents Chemother

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Antibiotic-resistant microbes, such as methicillin-resistant Staphylococcus aureus, seriously threaten human health. The outbreak of "superbugs" in recent years emphasizes once again the need for the development of new antimicrobial agents or resources. Antimicrobial peptides have an evident bactericidal effect against multidrug-resistant pathogens. In the present study, a new antimicrobial peptide, ctriporin, was cloned and characterized from the venom of the scorpion Chaerilus tricostatus, an animal which has not yet been explored for toxic peptide resources. The MICs of ctriporin against Staphylococcus aureus, Bacillus thuringiensis, Bacillus subtilis, Micrococcus luteus, and Candida albicans are 5 to 20 g/ml. Meanwhile, it MIC against clinical antibiotic-resistant bacterial strains is 10 g/ml. Furthermore, the potential for ctriporin to be used as a topical antibiotic for treating staphylococcal skin infections was investigated. External use of the peptide ctriporin dramatically decreased the bacterial counts and cured skin infections in mice. In addition, ctriporin demonstrates antimicrobial efficacy via the bactericidal mechanism of rapid cell lysis. Together, these results suggest the potential of developing ctriporin as a new topical antibiotic.

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“…Basic molecular studies of mosquitoes reveal remarkable adaptations to facilitate hematophagy, reproduction, olfaction, and immune responses to pathogen challenge, as well as genetic bases for insecticide resistance (Attardo et al, 2005;Enayati et al, 2005;Meister et al, 2004;Ranson and Hemingway, 2005;Ribeiro and Francischetti, 2003;Rützler and Zwiebel, 2005;Zwiebel and Takken, 2004). Both applied and basic science investigations benefit from advances in mosquito transgenesis technologies that include the discovery and development of transposable elements for germline integration of exogenous DNA, suitable marker genes such as fluorescent proteins, standardization of microinjection techniques, and characterization of promoters that drive tissue-, sex-, and stagespecific expression (Atkinson and James, 2002;Catteruccia et al, 2000Catteruccia et al, , 2005 ; Coates et al, 1999;Conde et al, 2000;Horn et al, 2002;Jasinskiene et al, 1998;Kim et al, 2004;Kokoza et al, 2001b;Lobo et al, 2006). Furthermore, the availability of whole genome sequence information for three mosquito species, Anopheles gambiae, Aedes aegypti, and Culex quinquefasciatus, allows global characterization of sequence conservation and genome structure through comparative and functional analyses by which patterns of evolution in gene and protein families are detected Nene et al, 2007;Waterhouse et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Gene Expression Studies in Mosquitoes

Chen¹,

Mathur²,

James³

2008

Advances in Genetics

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Research on gene expression in mosquitoes is motivated by both basic and applied interests. Studies of genes involved in hematophagy, reproduction, olfaction, and immune responses reveal an exquisite confluence of biological adaptations that result in these highly-successful life forms. The requirement of female mosquitoes for a bloodmeal for propagation has been exploited by a wide diversity of viral, protozoan and metazoan pathogens as part of their life cycles. Identifying genes involved in hostseeking, blood feeding and digestion, reproduction, insecticide resistance and susceptibility/ refractoriness to pathogen development is expected to provide the bases for the development of novel methods to control mosquito-borne diseases. Advances in mosquito transgenesis technologies, the availability of whole genome sequence information, mass sequencing and analyses of transcriptomes and RNAi techniques will assist development of these tools as well as deepen the understanding of the underlying genetic components for biological phenomena characteristic of these insect species.

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Scorpine, an anti‐malaria and anti‐bacterial agent purified from scorpion venom

Cited by 236 publications

References 14 publications

Beyond Lysozyme: Antimicrobial Peptides Against Malaria

Beyond Lysozyme: Antimicrobial Peptides Against Malaria

Ctriporin, a New Anti-Methicillin-Resistant Staphylococcus aureus Peptide from the Venom of the Scorpion Chaerilus tricostatus

Gene Expression Studies in Mosquitoes

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