SCO-spondin is evolutionarily conserved in the central nervous system of the chordate phylum

Biosynthesis and molecular biology of the secretory proteins of the subcommissural organ

“…Northern blot analyses showed the expression of a high molecular weight mRNA (10 -14 kb) only in the SCO (Fig. 9) (Gobron et al, 1999;Nualart et al, 1998). 3.…”

“…SCO-Spondin is evolutionarily conserved in the central nervous system. Gobron et al (1999) Immunohistochemical and electrophoretic analysis of the chick embryos SCO and metencephalic floor plate.…”

SCO-spondin, a glycoprotein of the subcommissural organ/reissner's fiber complex: Evidence of a potent activity on neuronal development in primary cell cultures

“…By Southern blot analysis, it was shown that the SCO-spondin gene was present as a single copy in the cattle genome (Creveaux et al, 1998). Also, an ortholog to the cattle SCO-spondin gene was revealed in the genome of various species of the chordate phylum (Gobron et al, 1999).…”

“…During recent years, we applied new methodological procedures, such as molecular biology, for the characterization of the SCO/RF secretions, which could help to uncover the putative biological function of these proteins. The analysis of the complete sequence of a novel glycoprotein called SCO-spondin, specifically expressed in the SCO Gobron et al, 1996Gobron et al, , 1999Creveaux et al, 1998), favours a developmental role for the SCO/RF complex through promoting axonal pathfinding. In parallel, we developed "in vitro" biological assays to test the role of Reissner's fiber and peptides derived from SCO-spondin on the differentiation of neuronal cells in primary cell cultures (Monnerie et al, 1995(Monnerie et al, , 1997a(Monnerie et al, ,b, 1998, and to correlate the molecular and functional features of SCO-spondin.…”

Human subcommissural organ, with particular emphasis on its secretory activity during the fetal life

“…Immunochemical and biochemical studies indicate that the nature of the glycoproteins secreted by the SCO is rather well conserved throughout evolution (Gobron et al, 1999;Rodríguez et al, 1984;Sterba et al, 1982). On the other hand, the SCO differentiates very early in ontogeny, and may represent one of the first secretory structures of the brain to develop (Naumann, 1986;Schöbitz et al, 1986).…”