SCO-Spondin Derived Peptide NX210 Induces Neuroprotection In Vitro and Promotes Fiber Regrowth and Functional Recovery after Spinal Cord Injury

Sakka, Laurent; Delétage, Nathalie; Lalloué, Fabrice; Duval, Amélie; Chazal, J.; Lemaire, Jean‐Jacques; Meiniel, Annie; Monnerie, Hubert; Gobron, Stéphane

doi:10.1371/journal.pone.0093179

Cited by 21 publications

(32 citation statements)

References 62 publications

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“…SSPO, an evolutionarily conserved gene in the central nervous systems of chordates, has a neuroprotective effect by promoting neuronal differentiation and growth in spinal cord injuries. 46 , 47 Our results showed that the downregulation of SSPO could be triggered by LPS infection in amphioxus. Therefore, we propose that the differential expression of these key neuro-related genes may be involved in neuro-inflammation, implying that a rudimentary, active neuro-immune system probably existed in basal chordates and acted as an ancient immune system in the ancestor of vertebrates.…”

Section: Discussionmentioning

confidence: 54%

Genome-wide gene expression analysis of amphioxus (Branchiostoma belcheri) following lipopolysaccharide challenge using strand-specific RNA-seq

et al. 2017

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Amphioxus is the closest living proxy for exploring the evolutionary origin of the immune system in vertebrates. To understand the immune responses of amphioxus to lipopolysaccharide (LPS), 5 ribosomal RNA (rRNA)-depleted libraries of amphioxus were constructed, including one control (0 h) library and 4 treatment libraries at 6, 12, 24, and 48 h post-injection (hpi) with LPS. The transcriptome of Branchiostoma belcheri was analyzed using strand-specific RNA sequencing technology (RNA-seq). A total of 6161, 6665, 7969, and 6447 differentially expressed genes (DEGs) were detected at 6, 12, 24, and 48 hpi, respectively, compared with expression levels at 0 h. We identified amphioxus genes active during the acute-phase response to LPS at different time points after stimulation. Moreover, to better visualize the resolution phase of the immune process during immune response, we identified 6057 and 5235 DEGs at 48 hpi by comparing with 6 and 24 hpi, respectively. Through real-time quantitative PCR (qRT-PCR) analysis of 12 selected DEGs, we demonstrated the accuracy of the RNA-seq data in this study. Functional enrichment analysis of DEGs demonstrated that most terms were related to defense and immune responses, disease and infection, cell apoptosis, and metabolism and catalysis. Subsequently, we identified 1330, 485, 670, 911, and 1624 time-specific genes (TSGs) at 0, 6, 12, 24, and 48 hpi. Time-specific terms at each of 5 time points were primarily involved in development, immune signaling, signal transduction, DNA repair and stability, and metabolism and catalysis, respectively. As this is the first study to report the transcriptome of an organism with primitive immunity following LPS challenge at multiple time points, it provides gene expression information for further research into the evolution of immunity in vertebrates.

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Section: Discussionmentioning

confidence: 54%

Genome-wide gene expression analysis of amphioxus (Branchiostoma belcheri) following lipopolysaccharide challenge using strand-specific RNA-seq

et al. 2017

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“…The homologous segment contains vWFD, C8, TIL and LDLrA domains, while the remainder features numerous copies of TSP-1, SCO-spondin region repeats (SCOR), TIL and a CTCK [ 76 ]. Interestingly, the TSP-1-rich segment from SCO-spondin that is absent in P-vulgata_3 is specifically implicated in promoting neuronal development [ 82 , 83 ], which appears to be a superfluous function for adhesive proteins. Furthermore, in situ hybridization localized P-vulgata_3 expression to the pedal sole, ruling out contamination as a possible source of SCO-spondin-like peptides.…”

Section: Discussionmentioning

confidence: 99%

Molecular insights into the powerful mucus-based adhesion of limpets ( Patella vulgata L.)

et al. 2020

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Limpets ( Patella vulgata L.) are renowned for their powerful attachments to rocks on wave-swept seashores. Unlike adult barnacles and mussels, limpets do not adhere permanently; instead, they repeatedly transition between long-term adhesion and locomotive adhesion depending on the tide. Recent studies on the adhesive secretions (bio-adhesives) of marine invertebrates have expanded our knowledge on the composition and function of temporary and permanent bio-adhesives. In comparison, our understanding of the limpets' transitory adhesion remains limited. In this study, we demonstrate that suction is not the primary attachment mechanism in P. vulgata ; rather, they secrete specialized pedal mucus for glue-like adhesion. Through combined transcriptomics and proteomics, we identified 171 protein sequences from the pedal mucus. Several of these proteins contain conserved domains found in temporary bio-adhesives from sea stars, sea urchins, marine flatworms and sea anemones. Many of these proteins share homology with fibrous gel-forming glycoproteins, including fibrillin, hemolectin and SCO-spondin. Moreover, proteins with potential protein- and glycan-degrading domains could have an immune defence role or assist degrading adhesive mucus to facilitate the transition from stationary to locomotive states. We also discovered glycosylation patterns unique to the pedal mucus, indicating that specific sugars may be involved in transitory adhesion. Our findings elucidate the mechanisms underlying P. vulgata adhesion and provide opportunities for future studies on bio-adhesives that form strong attachments and resist degradation until necessary for locomotion.

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“…SCO-spondin is expressed in the subcommissural organ (SCO) and plays a role in neuronal development [ 51 ]. It also promotes commissural fiber regrowth and functional recovery after spinal cord injury [ 52 ]. The DNA polymerase ε catalytic subunit encoded by Pole contributes to DNA repair and chromosomal DNA replication [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

RNA Sequencing Analysis Reveals Interactions between Breast Cancer or Melanoma Cells and the Tissue Microenvironment during Brain Metastasis

Sato

Nakano

Hosonaga

et al. 2017

BioMed Research International

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Metastasis is the main cause of treatment failure and death in cancer patients. Metastasis of tumor cells to the brain occurs frequently in individuals with breast cancer, non–small cell lung cancer, or melanoma. Despite recent advances in our understanding of the causes and in the treatment of primary tumors, the biological and molecular mechanisms underlying the metastasis of cancer cells to the brain have remained unclear. Metastasizing cancer cells interact with their microenvironment in the brain to establish metastases. We have now developed mouse models of brain metastasis based on intracardiac injection of human breast cancer or melanoma cell lines, and we have performed RNA sequencing analysis to identify genes in mouse brain tissue and the human cancer cells whose expression is associated specifically with metastasis. We found that the expressions of the mouse genes Tph2, Sspo, Ptprq, and Pole as well as those of the human genes CXCR4, PLLP, TNFSF4, VCAM1, SLC8A2, and SLC7A11 were upregulated in brain tissue harboring metastases. Further characterization of such genes that contribute to the establishment of brain metastases may provide a basis for the development of new therapeutic strategies and consequent improvement in the prognosis of cancer patients.

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SCO-Spondin Derived Peptide NX210 Induces Neuroprotection In Vitro and Promotes Fiber Regrowth and Functional Recovery after Spinal Cord Injury

Cited by 21 publications

References 62 publications

Genome-wide gene expression analysis of amphioxus (Branchiostoma belcheri) following lipopolysaccharide challenge using strand-specific RNA-seq

Genome-wide gene expression analysis of amphioxus (Branchiostoma belcheri) following lipopolysaccharide challenge using strand-specific RNA-seq

Molecular insights into the powerful mucus-based adhesion of limpets ( Patella vulgata L.)

RNA Sequencing Analysis Reveals Interactions between Breast Cancer or Melanoma Cells and the Tissue Microenvironment during Brain Metastasis

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