Sclerotium rolfsii lectin expressed in tobacco confers protection against Spodoptera litura and Myzus persicae

Vanti, Gulamnabi L.; Vishwanathreddy, H; Venkat, Hemalatha; Bhat, Ganapati; Padmanabhan, Venkatesh; Jayaprakash, N.S.; Hiremath, Vamadevaiah; Katageri, I. S.; Gonchigar, Sathisha J.; Swamy, Bale M.

doi:10.1007/s10340-015-0704-9

Cited by 5 publications

(2 citation statements)

References 51 publications

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“…Rhizoctonia solani agglutinin (RSA; β-trefoil-type lectin) and S. rolfsii lectin (SRL; actinoporin-type lectin), which show substantial toxicity against agriculturally important herbivorous insects such as cotton leafworms Spodoptera littoralis and S. litura [ 41 , 42 ]. Interestingly, SRL-expressing plants showed also resistance to sucking and chewing insects S. litura and Myzus persicae [ 43 ]. Accordingly, SRL-transgenic cotton plants showed high resistance to Aphis gossypii (69 % reduction in population) and S. litura (100 % larval mortality) [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Marasmius oreades agglutinin enhances resistance of Arabidopsis against plant-parasitic nematodes and a herbivorous insect

et al. 2021

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Background Plant-parasitic nematodes and herbivorous insects have a significant negative impact on global crop production. A successful approach to protect crops from these pests is the in planta expression of nematotoxic or entomotoxic proteins such as crystal proteins from Bacillus thuringiensis (Bt) or plant lectins. However, the efficacy of this approach is threatened by emergence of resistance in nematode and insect populations to these proteins. To solve this problem, novel nematotoxic and entomotoxic proteins are needed. During the last two decades, several cytoplasmic lectins from mushrooms with nematicidal and insecticidal activity have been characterized. In this study, we tested the potential of Marasmius oreades agglutinin (MOA) to furnish Arabidopsis plants with resistance towards three economically important crop pests: the two plant-parasitic nematodes Heterodera schachtii and Meloidogyne incognita and the herbivorous diamondback moth Plutella xylostella. Results The expression of MOA does not affect plant growth under axenic conditions which is an essential parameter in the engineering of genetically modified crops. The transgenic Arabidopsis lines showed nearly complete resistance to H. schachtii, in that the number of female and male nematodes per cm root was reduced by 86–91 % and 43–93 % compared to WT, respectively. M. incognita proved to be less susceptible to the MOA protein in that 18–25 % and 26–35 % less galls and nematode egg masses, respectively, were observed in the transgenic lines. Larvae of the herbivorous P. xylostella foraging on MOA-expression lines showed a lower relative mass gain (22–38 %) and survival rate (15–24 %) than those feeding on WT plants. Conclusions The results of our in planta experiments reveal a robust nematicidal and insecticidal activity of the fungal lectin MOA against important agricultural pests which may be exploited for crop protection.

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Section: Discussionmentioning

confidence: 99%

Marasmius oreades agglutinin enhances resistance of Arabidopsis against plant-parasitic nematodes and a herbivorous insect

et al. 2021

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“…These drugs' therapeutic index is also very narrow so even a slight accumulation of these drugs can cause lethal effects on the body. And SRL with its highly specific nature will not cause this issue thereby decreasing its toxicity level [144].…”

Section: Lectins As Diagnostic and Therapeutic Agentsmentioning

confidence: 99%

Lectins as a promising therapeutic agent for breast cancer: A review

Singh,

Agrawal,

Gupta

et al. 2024

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Efficient treatment of cancer has been a subject of research by scientists for many years. Current treatments for cancer, such as radiotherapy, chemotherapy and surgery have been used in traditional combination therapy, but they have major setbacks like non-specificity, non-responsiveness in certain cancer types towards treatment, tumor recurrence, etc. Epidemiological data has shown that breast cancer accounts for 14% of cancer cases occurring in Indian women. In recent years, scientists have started to focus on the use of natural compounds like lectins obtained from various sources to counter the side effects of traditional therapy. Lectins like Sambucus nigra Agglutinin, Maackia amurensis lectin, Okra lectins, Haliclona caerulea lectin, Sclerotium rolfsii lectin, etc., have been discovered to have both diagnostic and therapeutic potential for breast cancer patients. Lectins have been found to have inhibitory effects on various cancer cell activities such as neo-angiogenesis, causing cell cycle arrest at the G1 phase, and inducing apoptosis. The major idea behind the use of lectins in cancer diagnostics and therapeutics is their capability to bind to glycosylated proteins that are expressed on the cell surface. This review focuses on an exploration of the roles of post-translational modification in cancer cells, especially glycosylation, and the potential of lectins in cancer diagnosis and therapeutics.

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