SCL, GATA‐2 and Lmo2 expression in neurogenesis

Abstract

SCL, Lmo2 and GATA factors form common transcription complexes during hematopoietic differentiation. The overlapping expression of SCL with GATA-2 and GATA-3 in the developing brain indicated that these factors might collaborate also in the course of neural tissue differentiation. The expression pattern of Lmo2 in the developing CNS, however, is not well understood. Here, we show that neural cells in the early embryonic chick mid- and hindbrain express SCL and GATA-2, while Lmo2 is expressed only in vascular elements. The lack of Lmo2 transcripts in neural cells demonstrated that SCL and GATA-2 cannot form common complexes with Lmo2 in the developing brain. In the course of neural tissue genesis, GATA-2 mRNA appeared prior to the SCL transcript. While GATA-2 expression decreased with maturation, SCL expression persisted at a high level also in post-neurogenic periods. The temporal pattern of SCL and GATA-2/3 expression was investigated also in vitro, in the course of induced neurogenesis by NE-4C neural stem cells. While GATA-2 expression increased from the very beginning of differentiation, SCL expression appeared only in more differentiated cells expressing proneural genes. GATA-3 expression, on the other hand, was detected only in advanced stages of the neuronal maturation, which were characterised by the activation of the Math2 neuronal gene. Similarly to the hematopoietic differentiation, GATA-2 expression precedes the activation of both SCL and GATA-3, and may play roles in the activation of the SCL gene in neuronal development. In contrast to hematopoietic differentiation, however, our results failed to demonstrate co-assembling of GATA factors or SCL with Lmo2. While overlapping expression of GATA-2/3 and SCL was detected, Lmo2 activation could not be demonstrated in neural cells in the investigated period of neuronal development.

Temporal regulation of Cre‐recombinase activity in Scl‐positive neurons of the central nervous system

“…Our finding underlines the previously reported observation that Scl expression is restricted to postmitotic neuroprogenitor cells of the developing brain (Herberth et al, 2005;van Eekelen et al, 2003).…”

Retinoid Machinery in Distinct Neural Stem Cell Populations with Different Retinoid Responsiveness

“…2B-D Undifferentiated NE-4C cells display many embryonic stem cell features (Oct4, Nanog) with complete inactivity of tissue-specifying bHLH (Ngn2, Mash1, Math2) or radial glia specifying (Pax6, RC2) markers [32,57,58]. Their neural commitment is indicated by the expression of Sox2 [59] and by the fact that they could develop exclusively to neural cell types [60]. The lack of Oct4 and Nanog and the presence of RGl (RC2, GLAST) immunoreactivities together with the expression of proneural bHLH (Ngn and Mash1) genes indicate that noninduced RGl cells represent a more advanced progenitor state than NE-4C cells [35].…”

The Transcription Factor LMO2 Is a Robust Marker of Vascular Endothelium and Vascular Neoplasms and Selected Other Entities

“…In developing chick brain, LMO2 mRNA is limited to the vasculature, 15 although in adult mice, LMO2 mRNA expression has been demonstrated in specific portions of the thalamus and hippocampus. 16 We confirmed that in human cerebral tissue, in fetuses and adults, LMO2 protein expression is largely limited to the vasculature.…”