Scion-to-Rootstock Mobile Transcription Factor CmHY5 Positively Modulates the Nitrate Uptake Capacity of Melon Scion Grafted on Squash Rootstock

Hou, Shuan; Zhu, Yulei; Wu, Xiaofang; Xin, Ying; Guo, Jieying; Wu, Fang; Yu, Hanqi; Sun, Zhizeng; Xu, Chuanqiang

doi:10.3390/ijms24010162

Cited by 6 publications

(4 citation statements)

References 86 publications

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“…In the other hand, the shoot-derived auxin and ELONGATED HYPOCOTYL5 (HY5) might also function as the shoot-root mobile signal to balance the shoot-root growth by coupling shoot growth and carbon assimilation with root growth and nutrient uptake (Ljung et al, 2015;Chen et al, 2016). In the melon-pumpkin grafts, the scion-to-rootstock mobile HY5 signal positively modulated the nitrate uptake capacity of rootstock roots (Hou et al, 2022). Also, and for obtaining the energy necessary for meristem proliferation (Xiong et al, 2013;Li et al, 2017a).…”

Section: Discussionmentioning

confidence: 99%

Unveiling the mechanism of source‐sink rebalancing in cucumber‐pumpkin heterografts: the buffering roles of rootstock cotyledon

Pu,

Wen,

Sun

et al. 2024

Physiologia Plantarum

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Grafting onto pumpkin rootstock is widely applied in cucumber production to improve growth and yield, as well as to overcome soil‐borne diseases and enhance resistance to abiotic stresses. In this study, we constructed the cucumber‐pumpkin heterografts with the one‐cotyledon grafting method, and examined the effects of heterografting on biomass allocation and sugar partitioning, with cucumber and pumpkin self‐grafts used as control. Compared with cucumber self‐grafts, heterografting onto pumpkin rootstock promoted photosynthesis in cucumber scion, and led to higher sucrose contents in the 1st true leaf (source) and newly emerged leaf (sink). Thereby, the scion part of heterografts accumulated more biomass than cucumber self‐grafts. In contrast, when compared to pumpkin self‐grafts, grafting with cucumber scion reduced root vigor and biomass but promoted cotyledon growth in pumpkin rootstock. The roots (sink) of heterografts contained less sucrose and hexoses, and showed reduced sucrose synthase (SuSy) and hexokinase (HXK) activities. However, the rootstock cotyledon (source) contained more sucrose and starch, and showed higher activities of HXK, cell‐wall invertase (CWIN), and enzymes for starch synthesis and degradation. Furthermore, removal or shade of rootstock cotyledon led to reduced growth of root and scion. Silencing of CmoMEX1a gene in rootstock cotyledon inhibited maltose export and reduced root growth of heterografts. These results indicated that rootstock cotyledon, especially its starch content, played a buffering role in the growth regulation of cucumber‐pumpkin heterografts. Taken together, our results provided a major contribution to our understanding of source‐sink sugar partitioning and scion‐rootstock growth balancing in cucumber‐pumpkin heterografts.

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Section: Discussionmentioning

confidence: 99%

Unveiling the mechanism of source‐sink rebalancing in cucumber‐pumpkin heterografts: the buffering roles of rootstock cotyledon

Pu,

Wen,

Sun

et al. 2024

Physiologia Plantarum

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“…For example, along with the cultivation, participate soil organic-matter changes accordingly [23]. Scion and rootstock transportation modulates nitrate uptake capacity [24]. Biomass partitioning of Macadamia with high manganese and low phosphorus concentrations showed that dry weight of roots, stems, branches and leaves accounted for 14-20%, 19-30%, 36-52%, and 12-18% of total plant weight, respectively [17].…”

Section: Discussionmentioning

confidence: 99%

Effects of Substrate Composition on the Growth Traits of Grafting Seedling in Macadamia Nuts

Tan,

Zhou,

et al. 2024

Preprint

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Macadamia nut plantings in China are expanding year by year. In order to further promote the superior varieties, this study analyzed the effects of different varieties of rootstock and scion on the survival rate as well as the effects of 18 different ratios of substrate composition on seedling growth were analyzed. The results showed that: The survival rate of HAES788 varieties as rootstock and Guire No.1 as scion was the highest, reaching 96%. The optimal grafting time in December was better than that in March. Substrate formulations 12, 13, 15 and 16 had agglomerated soil and well-developed root systems compared to the controls. Plant height, stem diameter, leaf length, leaf width, and dry weight of aboveground and underground parts were significantly higher than those of the control. In addition, the substrate formulations 12, 13, 15 and 16 significantly improved the organic matter, total nitrogen, and total potassium content of the substrate soils, but little on total phosphorus content. All in all, macadamia grafting times are best in December, with HAES788 and Guire No.1 being the best rootstock and scion. Substrate formulations 12, 13, 15 and 16 are the optimal formula. This study provides a solid foundation to produce high-quality macadamia seedlings.

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“…Since it is known that the scion can also affect the rootstock [ 78 , 79 ], we analyzed the possible movement in both directions. β-glucuronidase activity was stable in the transgenic parts of the grafted plants for 10 years in the greenhouse and in the field, but no transfer through grafting in any direction was detected.…”

Section: Discussionmentioning

confidence: 99%

Impact of Intron and Retransformation on Transgene Expression in Leaf and Fruit Tissues of Field-Grown Pear Trees

Lebedev

2023

IJMS

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Stable and high expression of introduced genes is a prerequisite for using transgenic trees. Transgene stacking enables combining several valuable traits, but repeated transformation increases the risk of unintended effects. This work studied the stability and intron-mediated enhancement of uidA gene expression in leaves and different anatomical parts of pear fruits during field trials over 14 years. The stability of reporter and herbicide resistance transgenes in retransformed pear plants, as well as possible unintended effects using high-throughput phenotyping tools, were also investigated. The activity of β-glucuronidase (GUS) varied depending on the year, but silencing did not occur. The uidA gene was expressed to a maximum in seeds, slightly less in the peel and peduncles, and much less in the pulp of pear fruits. The intron in the uidA gene stably increased expression in leaves and fruits by approximately twofold. Retransformants with the bar gene showed long-term herbicide resistance and exhibited no consistent changes in leaf size and shape. The transgenic pear was used as rootstock and scion, but grafted plants showed no transport of the GUS protein through the graft in the greenhouse and field. This longest field trial of transgenic fruit trees demonstrates stable expression under varying environmental conditions, the expression-enhancing effect of intron and the absence of unintended effects in single- and double-transformed woody plants.

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Scion-to-Rootstock Mobile Transcription Factor CmHY5 Positively Modulates the Nitrate Uptake Capacity of Melon Scion Grafted on Squash Rootstock

Cited by 6 publications

References 86 publications

Unveiling the mechanism of source‐sink rebalancing in cucumber‐pumpkin heterografts: the buffering roles of rootstock cotyledon

Unveiling the mechanism of source‐sink rebalancing in cucumber‐pumpkin heterografts: the buffering roles of rootstock cotyledon

Effects of Substrate Composition on the Growth Traits of Grafting Seedling in Macadamia Nuts

Impact of Intron and Retransformation on Transgene Expression in Leaf and Fruit Tissues of Field-Grown Pear Trees

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