Scintigraphic tracking of 99mTechnetium-labelled equine peripheral blood-derived mesenchymal stem cells after intravenous, intramuscular, and subcutaneous injection in healthy dogs

Beerts, Charlotte; Brondeel, Carlien; Pauwelyn, Glenn; Depuydt, Eva; Tack, Liesa; Duchateau, Luc; Xu, Yangfeng; Saunders, Jimmy; Peremans, Kathelijne; Spaas, Jan H.

doi:10.1186/s13287-021-02457-9

Cited by 8 publications

(9 citation statements)

References 23 publications

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“…In line with the observation in recent studies of our group ( 16 ), no clinical abnormalities or ectopic tissue formations were detected throughout the study. Additionally, after study completion, the investigator remained in contact with the dog owner and no clinical abnormalities were reported since.…”

Section: Discussionsupporting

confidence: 92%

“…Our group recently reported the tracking of radiolabelled ePB-MSCs following intravenous, intramuscular and subcutaneous administration in healthy dogs. In that study, radiopharmaceutical uptake was predominantly seen in the heart, lung, liver and bladder ( 16 ). To the author's knowledge, no study has reported the tracking of MSCs in dogs suffering from orthopedic lesions.…”

Section: Introductionmentioning

confidence: 86%

“…The technique of 99m Tc labelling the ePB-MSCs was described in a previous study performed by our group ( 16 ). In a first step, stannous chloride powder (Sigma Aldrich, US) was liquefied in sterile basic water (pH 8.5).…”

Section: Methodsmentioning

confidence: 99%

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Homing of radiolabelled xenogeneic equine peripheral blood-derived MSCs towards a joint lesion in a dog

et al. 2022

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Osteoarthritis (OA) is a highly prevalent condition in dogs, causing a substantial reduction in quality of life and welfare of the animals. Current disease management focusses on pain relief but does not stop the progression of the disease. Therefore, mesenchymal stem cells (MSCs) could offer a promising disease modifying alternative. However, little is known about the behavior and the mode of action of MSCs following their administration. In the current case report, 99mTechnetium labelled xenogeneic equine peripheral blood-derived MSCs were intravenously injected in a 9 year old dog suffering from a natural occurring cranial cruciate ligament rupture. The biodistribution of the MSCs was evaluated during a 6-h follow-up period, using a full body scintigraphy imaging technique. No clinical abnormalities or ectopic tissue formations were detected throughout the study. A radiopharmaceutical uptake was present in the liver, heart, lung, spleen, kidneys and bladder of the dog. Furthermore, homing of the radiolabelled MSCs to the injured joint was observed, with 40.61 % higher uptake in the affected joint in comparison with the healthy contralateral joint. Finally, a local radioactive hotspot was seen at a part of the tail of the dog that had been injured recently. The current study is the first to confirm the homing of xenogeneic MSCs to a naturally occurring joint lesion after IV administration.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 86%

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Homing of radiolabelled xenogeneic equine peripheral blood-derived MSCs towards a joint lesion in a dog

et al. 2022

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“…As an adult stem cell, they exist in organ stroma and connective tissue with strong proliferation ability [ 7 ]. When in a suitable environment, they can differentiate into a variety of cells, such as osteoblasts [ 8 , 9 ]. Therefore, they also show a certain multidirectional differentiation potential and can be separated from bone marrow and other tissues [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Human Adipose Derived Mesenchymal Stem Cells in Promoting Skin Wound Healing

Zhou

Wang

Yao³

et al. 2022

Journal of Healthcare Engineering

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Background. The aim of this pilot clinical study is to evaluate the efficacy of human adipose derived mesenchymal stem cells (HAMSCs) treatment for the wound healing with patients. Methods. This study was a clinical trial to investigate the efficacy of human adipose derived mesenchymal stem cells treatment for the wound healing with patients. 346 patients with skin wounds attending the central hospital of Yue Yang were enrolled in the study, setting in the period from January 2016 to January 2021. Patients were randomly allocated into two groups: experimental group received treatment with human adipose derived mesenchymal stem cells for each 10 cm2 of wound and control group received conventional dressing with normal saline for each 10 cm2 of wound. Results. No adverse events were recorded during the period of treatment. The granulation tissue coverage rate and thickness of granulation tissue after 10 days of treatment in experimental group were significantly improved compared with control group. Furthermore, the occurrence of bleeding of wound and suppurative wounds between two groups had significant difference ( P < 0.05 ). Conclusion. The data in this pilot study indicated that human adipose derived mesenchymal stem cells may be a safe and effective alternative therapy for wound healing. Moreover, larger, placebo-controlled, perspective studies are necessity to evaluate the efficacy and safety of human adipose derived mesenchymal stem cells treatment for wound healing patients.

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“…Mesenchymal stem cells (MSCs) are multipotent stromal cells from adult tissues that have the capability of differentiating in different cell lineages [ 7 ]. Umbilical Cord MSCs (UCMSCs) is one of the ideal sources of MSCs, which are attractive for clinical use [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

MiR-455-5p upregulation in umbilical cord mesenchymal stem cells attenuates endometrial injury and promotes repair of damaged endometrium via Janus kinase/signal transducer and activator of transcription 3 signaling

Sun¹,

Jiang

Chen

et al. 2021

Bioengineered

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Umbilical cord mesenchymal stem cells (UCMSCs) are regarded as an ideal source for clinical use. Increasing evidence has suggested that microRNAs (miRNAs) work as a crucial regulator in the development of plentiful diseases, including intrauterine adhesions (IUA). Herein, we investigated the specific impacts of UCMSCs overexpressing miR-455-5p in IUA. UCMSCs were cocultured with endometrial stromal cells (ESCs). Thirty-two female mice were divided into four different treated groups: sham, model, model + UCMSC-miR-NC and model + UCMSC-miR-455-5p. Mice in model groups were induced by uterine curettage. MiR-455-5p overexpressed UCMSCs facilitated the proliferation and cell cycle progression of ESCs according to 5-ethynyl-2′-deoxyuridine assay and flow cytometry analysis. Hematoxylin-eosin and Masson staining revealed that miR-455-5p upregulation in UCMSCs increased the number of endometrial glands and suppressed endometrial fibrosis in murine uterine tissues. Western blotting displayed that miR-455-5p overexpressed UCMSCs promoted the activation of Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) signaling in ESCs and murine uterine tissues. Mechanistically, miR-455-5p targeted 3’ untranslated region of suppressor of cytokine signaling 3 (SOCS3), which was confirmed by luciferase reporter assay. Reverse transcription quantitative polymerase chain reaction demonstrated that miR-455-5p was lowly expressed and SOCS3 was highly expressed in murine uterine tissues of IUA model. Moreover, Pearson correlation analysis showed that their expression was inversely correlated. Rescue assays suggested that inhibiting JAK/STAT3 signaling reversed effects of miR-455-5p on the behaviors of ESCs. The results indicated that miR-455-5p overexpression in UCMSCs helps to attenuate endometrial injury and repair damaged endometrium by activating SOCS3-mediated JAK/STAT3 signaling.

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Scintigraphic tracking of 99mTechnetium-labelled equine peripheral blood-derived mesenchymal stem cells after intravenous, intramuscular, and subcutaneous injection in healthy dogs

Cited by 8 publications

References 23 publications

Homing of radiolabelled xenogeneic equine peripheral blood-derived MSCs towards a joint lesion in a dog

Homing of radiolabelled xenogeneic equine peripheral blood-derived MSCs towards a joint lesion in a dog

Efficacy of Human Adipose Derived Mesenchymal Stem Cells in Promoting Skin Wound Healing

MiR-455-5p upregulation in umbilical cord mesenchymal stem cells attenuates endometrial injury and promotes repair of damaged endometrium via Janus kinase/signal transducer and activator of transcription 3 signaling

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