Scientific validation and formulation of three Indian Folklore medicinal plants

Ganapaty, S.; Maddi, Ramaiah; prudhivi, Ramakrishna; Reddy, Daka Nagarjuna

doi:10.1016/j.jopr.2013.08.012

Cited by 3 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to its anti-oxidant properties, we selected this medicinal plant to evaluate its effect on L-glutamate-induced damage in C6 cells. Ganapaty et al also reported the anti-oxidant and free radical scavenging properties of C. epithymum depend on the doses (22).…”

Section: Discussionmentioning

confidence: 94%

Cuscuta epithymum Murr. crude extract pre-conditioning inhibits cell apoptosis in glutamate-induced cytotoxic condition

Pourhadi

Niknam

Ghasemi

et al. 2022

Preprint

View full text Add to dashboard Cite

Cuscuta epithymum Murr. (C. epithymum), as a herbal medication, has shown anticancerous effects in some of in vitro studies, but its neuroprotective and anti-apoptotic possible effects have not been considered in research. Here, we aimed to show the protective effects of C. epithymum seeds crude extract and different fractions (n-hexane, dichloromethane, and methanol) on rat glioblastoma cells (C6) in L-glutamate exposure. Initially, the total phenolic content of C. epithymum crude extract and the fractions (all produced by maceration method) was determined. Subsequently, C6 cells were pre-treated with various doses of crude extract and fractions 24 hours before L-glutamate exposure. Likewise, C6 cells were treated with the same extract and fractions concentrations 24 hours after exposure L-glutamate. Besides, flow cytometry testing was used to prove that C. epithymum extract protects C6 cells from glutamate toxicity, as evidenced by morphological features, cell viability, and oxidative stress markers such as malondialdehyde (MDA) and superoxide dismutase (SODs). The findings suggested that crude extract has concentration-depended toxicity. It has the highest antioxidant activity to significantly increase C6 cell viability in cytotoxic conditions and probably plays the neuroprotective role in reducing cell death by decreasing MDA levels and increasing SODs levels in cells, showing lipid peroxidation prevention and free radical scavenging, respectively. In conclusion, these results suggest the protective role for C. epithymum crude extract against oxidative stress-induced damage. It showed propective attribute in our in vitro study ,although, further research is needed to prove the neuroprotective effects in in vivo oxidative conditions.

show abstract

Section: Discussionmentioning

confidence: 94%

Cuscuta epithymum Murr. crude extract pre-conditioning inhibits cell apoptosis in glutamate-induced cytotoxic condition

Pourhadi

Niknam

Ghasemi

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Due to its antioxidant properties, we selected this medicinal plant to evaluate its effect on L-glutamate-induced damage in C6 cells. Ganapathy et al also reported antioxidant and free radical scavenging properties of C. epithymum depend on the doses [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Cuscuta epithymum Murr. crude extract pre-conditioning protects C6 cells from L-glutamate-induced neurotoxicity

Pourhadi

Niknam

Ghasemi

et al. 2022

BMC Complement Med Ther

View full text Add to dashboard Cite

Background Cuscuta epithymum Murr. (C. epithymum), as an herbal medicine, has played an anti-cancerous role in various studies; however, its possible neuroprotective effects have been neglected. Here, we aimed to investigate the protective effects of C. epithymum seeds crude extract and different fractions on rat glioblastoma cells (C6) in L-glutamate oxidative condition. Methods Initially, the total phenolic content of C. epithymum crude extract and the fractions (all produced by maceration method) was determined. Subsequently, C6 cells were pre-treated with the various concentrations of crude extract and fractions 24 h before L-glutamate exposure. Likewise, C6 cells were treated with the same concentrations of crude extract and fractions 24 h after exposure to L-glutamate. The cell viability and morphology were compared in crude extract and fractions groups, then superoxide dismutase (SODs) activity, reactive oxygen species (ROS), and malondialdehyde (MDA) levels were measured. The flow cytometry test was used to study C. epithymum crude extract's effects on the cell cycle and also to quantify the apoptosis, necrosis, and live cells population in different groups. Results C. epithymum crude extract and fractions (hexanoic, dichloromethanolic, and methanolic) had concentration-dependent cytotoxicity (IC50:126.47, 2101.96, 140.97, and 218.96 µg/ml, respectively). The crude extract and methanolic fraction contained phenolic compounds (55.99 ± 2.795 and 50.80 ± 2.969 mg gallic acid/g extract), while in hexanoic and dichloromethanolic fractions, the phenolic content was undetectable. In the cell viability assay, in comparison to fractions, the crude extract showed a more protective effect against glutamate-induced oxidative condition (P < 0.0001). The crude extract increased the SODs activity (P < 0.001) and decreased MDA and ROS levels (P < 0.0001) in comparison to the glutamate group. The crude extract significantly increased the population of cells in G1 (from 63.04 to 76.29) and decreased the percentage of cells in G2 (from 11.56 to 6.7) and S phase (from 25.4 to 17.01). In addition, it decreased the apoptotic and necrotic cell populations (from 34 to 17.1) and also increased the percentage of live cells (from 66.8 to 83.4 percent) in the flow cytometry test. Conclusion C. epithymum crude extract plays a neuroprotective role by activating the defense mechanisms in cell against the oxidative condition.

show abstract

“…[22] Modern day lifestyle leads to numerous stress conditions, among which depression is a widely prevalent senile neurological disorder. It is mainly caused by decreased brain levels of monoamines like Noradrenaline, Dopamine and Serotonin.…”

Section: Discussionmentioning

confidence: 99%

Screening for Antidepressant-Like Effect of Methanolic Seed Extract of Avena Sativa using Animal Models

K¹,

Maddi²,

Nagaphani³

et al. 2014

Phcog J.

Self Cite

View full text Add to dashboard Cite

Depression affects about up to 20% of the population across the globe.The present study was designed to screen antidepressant activity of methanolic seed extract of Avena sativa (MSEAS). An in-vivo experimental methods were designed such as behavioral models like Forced swim test (FST), Tail suspension test (TST) and based on mechanism of action i.e., Antagonism of Apomorphine induced hypothermia on Swiss male albino mice. MSEAS 100 and 200 mg/kg, p.o were administered daily for 7 days. Fluoxetine 25mg/kg p.o was standard antidepressant drug in behavioral models and Desipramine 20mg/kg p.o in Apomorphine induced hypothermia.The methanolic extract produces a significant antidepressant effect in both FST and TST as they reduce the immobility. It was also found, effective in antagonizing or reversing hypothermia produced by apomorphine. The Anti-depressant activity of methanolic seed extract of Avena sativa was found to be significant at low doses (100mg/kg, po). The present study clearly demonstrated that Avena sativa exerts an antidepressant effect in these two behavioral models. The flavonoid components of MSEAS might be interacting with adrenergic system in mediating the anti depressant effect of Avena sativa.

show abstract

Scientific validation and formulation of three Indian Folklore medicinal plants

Cited by 3 publications

References 14 publications

Cuscuta epithymum Murr. crude extract pre-conditioning inhibits cell apoptosis in glutamate-induced cytotoxic condition

Cuscuta epithymum Murr. crude extract pre-conditioning inhibits cell apoptosis in glutamate-induced cytotoxic condition

Cuscuta epithymum Murr. crude extract pre-conditioning protects C6 cells from L-glutamate-induced neurotoxicity

Screening for Antidepressant-Like Effect of Methanolic Seed Extract of Avena Sativa using Animal Models

Contact Info

Product

Resources

About