Scientific and experimental substantiation of the technology for antigens of candida fungi purification

Rybalkin, M. V.; Стрельніков, Л. С.

doi:10.15587/2519-4852.2017.93905

ScienceRise: Pharmaceutical Science

2017

DOI: 10.15587/2519-4852.2017.93905

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Scientific and experimental substantiation of the technology for antigens of candida fungi purification

M. V. Rybalkin¹,

Л. С. Стрельніков²

Abstract: НАУКОВЕ ТА ЕКСПЕРИМЕНТАЛЬНЕ ОБГРУНТУВАННЯ ТЕХНОЛОГІЇ ОЧИЩЕН-НЯ АНТИГЕНІВ ГРИБІВ

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Cited by 2 publications

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Determination of Candida albicans proteins concentration by enzyme-linked immunosorbent assay method at subcutaneous introduction in candidiasis therapy

Rybalkin¹,

Khokhlenkova²,

Azarenko³

et al. 2020

PHAR

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Therapeutic effect of C. albicans proteins in concentrations 1, 2, 3, 4 and 5 mg/ml has been examined in white mice. Animals were infected i.p. with suspension of C. albicans strain CCM 335–867 in the amount of 20 million cells per 1 ml volume. After 5 days and repeatedly after 14 days mice were injected s.c. in the upper part of right hind paw with proteins of Candida cells of volume 0.2 ml. 14 days after each injection, the determination of the animal body protective functions has been carried out by the titer of specific antibodies during immunoassay. According to the data obtained, it has been found that in the s.c. administration, after the first and second injection with C. albicans protein of concentration 1, 2, 3, 4 and 5 mg/ml antibody titers increased 2 times, indicating that there is no stimulation of immune protection.

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Determination of Candida albicans proteins concentration by enzyme-linked immunosorbent assay method at subcutaneous introduction in candidiasis therapy

Rybalkin¹,

Khokhlenkova²,

Azarenko³

et al. 2020

PHAR

View full text Add to dashboard Cite

show abstract

Determination of Candida albicans fungus proteins concentration by Elisa method at Intramuscular Introduction in Candidiasis Therapy

Rybalkin

Diadiun

Хохленкова

et al. 2021

RJPT

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The objective of this work was to determine the C. albicans fungus protein concentration at intramuscular introduction in therapy of candidiasis. Therapeutic effect of C. albicans fungus proteins in concentrations 1, 2, 3, 4 and 5mg/mL has been examined in white mice. Animals were infected intraperitoneally with suspension of C. albicans fungus strain CCM 335-867 in the amount of 20 million cells per 1mL volume. After 5 days and repeatedly after 14 days mice were injected intramuscularly in the upper back hind paw with proteins of Candida fungus cells of volume 0.2mL. 14 days after each injection, the determination of the protective functions of the animal body has been carried out by the titer of specific C. albicans antibodies during immunoassay. According to the data obtained during studies on the treatment of candidiasis, it has been found that in the intramuscular route of administration after the first injection with C. albicans protein concentration of 3 mg/mL antibody titers increased 2 times, and after the second - 8 times. Proteins of C. albicans fungus at a concentration of 3 mg/mL activate the body's defence mechanisms.

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Scientific and experimental substantiation of the technology for antigens of candida fungi purification

Abstract: НАУКОВЕ ТА ЕКСПЕРИМЕНТАЛЬНЕ ОБГРУНТУВАННЯ ТЕХНОЛОГІЇ ОЧИЩЕН-НЯ АНТИГЕНІВ ГРИБІВ

Cited by 2 publications

References 3 publications

Determination of Candida albicans proteins concentration by enzyme-linked immunosorbent assay method at subcutaneous introduction in candidiasis therapy

Determination of Candida albicans proteins concentration by enzyme-linked immunosorbent assay method at subcutaneous introduction in candidiasis therapy

Determination of Candida albicans fungus proteins concentration by Elisa method at Intramuscular Introduction in Candidiasis Therapy

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