People with biological essentialist beliefs about social groups also tend to endorse biased beliefs about individuals in those groups, including stereotypes, prejudices, and intensified emphasis on the group. These correlations could be due to biological essentialism causing bias, and some experimental studies support this causal direction. Given this prior work, we expected to find that biological essentialism would lead to increased bias compared to a control condition and set out to extend this prior work in a new direction (regarding “value-based” essentialism). But although the manipulation affected essentialist beliefs and essentialist beliefs were correlated with stereotyping (Studies 1, 2a, and 2b), prejudice (Studies 2a), and group emphasis (Study 3), there was no evidence that biological essentialism caused these outcomes. Given these findings, our initial research question became moot, and the present work focuses on reexamining the relationship between essentialism and bias. We discuss possible moderators, reverse causation, and third variables.