Sciatic nerve repair using poly(ε‐caprolactone) tubular prosthesis associated with nanoparticles of carbon and graphene

Assaf, Kyl; Leal, Claudenete Vieira; Derami, Mariana Silveira; Duek, Eliana Aparecida de Rezende; Ceragioli, Helder José; Oliveira, Alexandre Leite Rodrigues de

doi:10.1002/brb3.755

Cited by 27 publications

(17 citation statements)

References 42 publications

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“…Nowadays, synthetic biocompatible and bioresorbable polymers are commonly used in constructing tubular structures for tissue regeneration [2,11,12]. Several works have demonstrated that poly(ε-caprolactone) (PCL) membranes can be successfully used as cell culture platforms for bio-hybrid neuronal models, small caliber blood vessel regeneration, and for the development of vascularized human hepatic tissue [13][14][15][16]. Also, a microfluidic perfusion system was recently developed using a blend of PCL and poly(dl-lactide-co-glycolide) (PLGA) made by freeze-coating a 3D-printed sacrificial template [17] as an ex vivo vascularized neural construct.…”

Section: Introductionmentioning

confidence: 99%

“…The PCL/graphene composites incorporate the benefits of PCL, a proven biodegradable and biocompatible material [ 25 ] and the ability of graphene to provide electrical conductivity that could modulate the bioelectrical activity of neural cells. So far, few works have fabricated PCL/graphene tubular 3D-scaffolds using solvent evaporation techniques [ 14 ] or 3D printing methodologies [ 26 ] for tissue engineering applications. In both cases, the electrically conductive 3D graphene scaffolds promoted peripheral nerve regeneration and remyelination after peripheral nerve injury.…”

Section: Introductionmentioning

confidence: 99%

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Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models

et al. 2020

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There is a huge interest in developing novel hollow fiber (HF) membranes able to modulate neural differentiation to produce in vitro blood–brain barrier (BBB) models for biomedical and pharmaceutical research, due to the low cell-inductive properties of the polymer HFs used in current BBB models. In this work, poly(ε-caprolactone) (PCL) and composite PCL/graphene (PCL/G) HF membranes were prepared by phase inversion and were characterized in terms of mechanical, electrical, morphological, chemical, and mass transport properties. The presence of graphene in PCL/G membranes enlarged the pore size and the water flux and presented significantly higher electrical conductivity than PCL HFs. A biocompatibility assay showed that PCL/G HFs significantly increased C6 cells adhesion and differentiation towards astrocytes, which may be attributed to their higher electrical conductivity in comparison to PCL HFs. On the other hand, PCL/G membranes produced a cytotoxic effect on the endothelial cell line HUVEC presumably related with a higher production of intracellular reactive oxygen species induced by the nanomaterial in this particular cell line. These results prove the potential of PCL HF membranes to grow endothelial cells and PCL/G HF membranes to differentiate astrocytes, the two characteristic cell types that could develop in vitro BBB models in future 3D co-culture systems.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models

et al. 2020

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“…The biodegradable poly(lactic acid) (PLA)/poly(ε‐caprolactone) (PCL) blend is a material already used in the biomedical field . However, the addition of nanoparticles such as CNT and graphene nanoplatelets (GN) can provide it with new properties, allowing for new areas of application, since they produce fully biodegradable polymer blends combining good stiffness, strength, ductility, flammability, and electrical properties . However, as previously mentioned, the control of selective nanoparticle localization is important to define the blend's final properties.…”

Section: Introductionmentioning

confidence: 99%

Mixing‐sequence controlled selective localization of carbon nanoparticles in PLA/PCL blends

Aguiar

Decol

Pachekoski

et al. 2018

Polymer Engineering & Sci

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This work proposes modifying the adding sequence of components during the production of poly(lactic acid) (PLA) and poly(ε‐caprolactone) (PCL) ternary systems with multiwalled carbon nanotubes (CNT) or graphene nanoplatelets (GN), in order to analyze the nanoparticles’ selective localization in terms of the competition between different factors, such as kinetics, thermodynamics, rheology, and geometry. In the studied system, the PLA's viscosity is higher than the PCL's and the difference in interactions between the CNT or the GN/PLA and the CNT or the GN/PCL is small, meaning the viscosity effects could dominate. However, other factors, such as thermodynamics and geometry, can be observed to compete with the rheological factor, depending on the mixing sequences. Depending on the processing time, a competition between thermodynamics and rheological factors could be observed when the nanoparticles had contact first with the thermodynamically favored phase. The competition between geometrical and rheological factors was observed in the presence of GN in the PLA phase, in the system in which the GN was added first to the PLA phase, which is the phase of lowest affinity. POLYM. ENG. SCI., 59:323–329, 2019. © 2018 Society of Plastics Engineers

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“…They proved effective in improving nerve regeneration of a nerve gap [26,27] or nerve coaptation site wrapping [28]. PCL is also combined with other particles, that may facilitate nerve regeneration, such as laminin [29] graphene [30], carbon nanotubes [31], small porcine intestine submucosa [32]. Thus, P(LLA-CL)-COL-PANI may serve as an effective platform for further enhancements and modi cations yet exhibiting considerable proneuroregenerative effect.…”

Section: Discussionmentioning

confidence: 99%

Bioactive Nanofiber-based Conduits in a Peripheral Nerve Gap Management- an Animal Model Study.

Dębski

Kijeńska‐Gawrońska

Zołocińska

et al. 2020

Preprint

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Background: Peripheral nerve injuries (PNI) are trauma with severe squeals for patients’ quality of life. Currently, the gold standard of nerve reconstruction relies on primary coaptation. However, in case of a nerve gap, a reconstructive material for bridging is needed. Nerve conduits are an increasingly popular solution alternative to autografts or processed nerved allografts. The aim of the study was to examine the efficiency of a novel bioactive nanofiber-based tubular scaffold made of poly (L-lactic acid)-co-poly(ϵ-caprolactone), collagen, polyaniline and enriched with adipose-derived stem cells (ASCs) as a nerve conduit in a rat model.Methods: Poly (L-lactic acid)-co-poly(ϵ-caprolactone) scaffold was optimized and enriched with collagen (COL) and polyaniline (PANI) to create final (P(LLA-CL)-COL-PANI), tubular-shaped scaffold, manufactured with electrospinning technology. Parallelly, adipose tissue from 10 Lewis rats was harvested for ASC culture. 28 inbred male Lewis rats were divided into four even groups. Each animal underwent sciatic nerve transection and excision of a 10 mm nerve trunk fragment. In group A, nerve gap remained untouched, in B excised trunk was rotated and used as an autograft, in C nerve stumps were secured with P(LLA-CL)-COL-PANI conduit. In the D group gap was reconstructed with P(LLA-CL)-COL-PANI conduit enriched with ASCs. After 6-months of observation rats were sacrificed. Gastrocnemius muscles (operated and non-operated side) and reconstructed sciatic nerves were harvested for analyses. Muscles were weighted and underwent histological analysis. Nerves were processed and stained immunohistochemically with NF-200 to be analyzed with dedicated software for nerve fiber count. Results: No signs of rejection or excessive fibrosis was noted. Muscle mass ratio was highest in group B (0.77±0.05), then in C (0.74±0,04) and D (0.67± 0.07). Group A showed advanced atrophy of the muscle, each intervention prevented muscle mass decrease (p<0.0001), however, ASC addition decreased efficiency vs autograft (p<0.05). Nerve fiber count revealed a superior effect in the nerve fiber density observed in the group with the use of conduit vs autograft (D vs B p<0.0001, C vs B p<0.001). ASC added to the conduit decreased perineurium hyperplasia.Conclusion: P(LLA-CL)-COL-PANI conduits with ASC showed promising results in managing nerve gap by decreasing muscle atrophy and providing a favorable environment for peripheral nerve regeneration.

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Sciatic nerve repair using poly(ε‐caprolactone) tubular prosthesis associated with nanoparticles of carbon and graphene

Abstract: Introduction: Injuries to peripheral nerves generate disconnection between spinal neu-

Cited by 27 publications

References 42 publications

Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models

Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models

Mixing‐sequence controlled selective localization of carbon nanoparticles in PLA/PCL blends

Bioactive Nanofiber-based Conduits in a Peripheral Nerve Gap Management- an Animal Model Study.

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