Sciatic nerve regeneration in rats subjected to ketogenic diet

Abstract

Regeneration of sciatic nerves was improved in KD - preconditioned rats. These results suggest a neuroprotective effect of KD on peripheral nerves.

Fasting and cancer: molecular mechanisms and clinical application

“…Clinical studies indicate that KDs probably have no substantial therapeutic activity when used as single agents in patients with cancer and suggest that potential benefits of these diets should be sought in combination with other approaches, such as chemotherapy, radiotherapy, antiangiogenic treatments, PI3K inhibitors and FMDs 72,73 . KDs were reported to have neuroprotective effects in peripheral nerves and in the hippocampus 87,88 . However, it remains to be established whether KDs also have pro-regenerative effects similar to fasting or FMDs (BOX 1) and whether KDs also can be used to protect living mammals from the toxicity of chemotherapy.…”

The course of recovery of locomotor function over a 10‐week observation period in a rat model of femoral nerve resection and autograft repair

“…The rat sciatic nerve model is the most frequently used rodent model to investigate peripheral nerve regeneration (Irintchev, ) after axonotmesis (Chen et al, ; Liskiewicz et al, ; Neerven et al, ) or neurotmesis (Bozkurt et al, ; Madison, Zomorodi, & Robinson, ; Varejao et al, ). Methods to assess functional recovery in this particular model via gait analysis are quite numerous (Bozkurt, Tholl, et al, ; Lin, Pan, Hom, Sabbahi, & Shenaq, ; Monte‐Raso, Barbieri, Mazzer, Fonseca, & Barbieri, ; Varejao, Melo‐Pinto, Meek, Filipe, & Bulas‐Cruz, ), but evaluation of locomotor ability via CatWalk is one of the most frequently used methods (Bozkurt et al, ; Deumens et al, ; Vrinten & Hamers, ).…”

The Role of Dietary Nutrients in Peripheral Nerve Regeneration

“…Overall, the lack of standardisation among all three studies in terms of diet composition, employed animal model, and timing of dietary intervention, as can also be derived from Table 1, precludes a synthesis of their outcomes as a whole. However, the coherent positive results reported by Liśkiewicz et al and Li et al [22,24] are noteworthy, because they performed their studies on the same animal model with similar timing and diet composition.…”

“…De Angelis et al, 2020 [25] Chronic constriction injury of sciatic nerve in mouse (CD1 male mice 12 months old) 40% less of daily consumption for 7 days after CCI Coccurello et al, 2018 [26] Chronic constriction injury (CCI) of sciatic nerve (Wild-type CD1 male mice, Ambra1+ transgenic CD1 male mice) 40% less of daily consumption for 7 days after CCI High-fat diet Song et al, 2018 [27] Plantar incision (Long-Evans rats) 40% fat administrated with different timing: a group received a high-fat diet for 6 weeks before injury and 2 weeks after, another group was fed with high-fat diet for 7 weeks and then switched back to standard diet for 2 weeks before to plantar incision, while in the last group, only male rats were switched to high-fat diet 1 week before injury Bekar et al, 2014 [28] Sciatic nerve crush injury (Sprague-Dawley rats) 40% fat for 3 months (started before injury and ended 4 weeks after injury) Liskiewicz et al [24] explored the impact of KD administered before and/or after sciatic nerve crush and observed that regenerating nerves in the preconditioned KD group showed the most similar morphometrical parameters to those of uninjured rats, including myelin thickness, fibre density, and fibre diameter. However, functional analysis based on the CatWalk test showed no significant differences between rats fed with KD and rats subjected to a standard diet.…”