Sciatic Nerve of Diabetic Rat Treated With Epoetin Delta: Effects on C-Fibers and Blood Vessels Including Pericytes

Abstract

In diabetes mellitus (DM) reduced motor and sensory properties of peripheral nerves are linked with the dysfunction of neural vasculature. We investigated C-fibers and microvessels of sciatic nerve of normal, DM, and DM + epoetin delta-treated rats. C-fibers immunoreactive for calcitonin gene-related peptide (CGRP), tyrosine hydroxylase (TH), epoetin receptor (EpoR), and common beta receptor subunit of the interleukin 3 receptor (IL-3Rbeta) were present in all rats, whereas in DM and epoetin-treated rats C-fibers also showed neuronal (nNOS) and inducible (iNOS) nitric oxide synthases. The cross-sectional area of CGRP-positive C-fibers was decreased in DM, but it recovered after epoetin treatment. In all conditions, vascular endothelium showed scarce immunolabeling for endothelial nitric oxide synthase (eNOS); the profound immunoreactivity for eNOS, EpoR, and IL-3Rbeta was in pericytes. Some perivascular autonomic nerves were damaged and IL-3Rbeta positive. Findings are discussed in terms of declined sensory conduction velocity in DM, its improvement after epoetin treatment, and the possible vascular contribution to these phenomena.

Hemoglobin induced NO/cGMP suppression Deteriorate Microcirculation via Pericyte Phenotype Transformation after Subarachnoid Hemorrhage in Rats

“…Although the main source of intense NO after brain ischemia/reperfusion injury are the microvessels 32 , eNOS still mostly came from endothelium and nerve ending. However, Zakoji N, et al and Loesch A, et al demonstrated that pericyte could also express eNOS 33 34 , which could be one of the reasons that pericyte generates NO under inflammatory condition 35 36 . In addition to ipsilateral cortex homogenates, we analyzed the expression and activity of eNOS in cultured pericytes as we showed in Fig.…”

Flipping the molecular switch for innate protection and repair of tissues: Long-lasting effects of a non-erythropoietic small peptide engineered from erythropoietin

“…A host of tissues and cells under stress have been shown to express and co-localize EPOR and βcR, including the central (Brines et al, 2004) and peripheral (Loesch et al, 2010) nervous systems, retina (Colella et al, 2011), heart (Xu et al, 2009), kidney (Kitamura et al, 2008), skeletal muscle (Collino et al, 2014), the endothelium (Su et al, 2011), as well as macrophages and bone marrow derived mesenchymal cells (Bohr et al, 2015).…”

Immunoreactive Endothelin-1 and Endothelin A Receptor in Basilar Artery Perivascular Nerves of Young and Adult Capybaras

“…Similar changes, e.g. cytoplasmic vacuolization, may be observed in Schwann cells in diabetic peripheral nerve pathology [Loesch et al, 2010b] or the occurrence of myelin-like figures in Wallerian-like degeneration; the latter may in fact occur without the presence of macrophages to remove debris, e.g. myelin [Fernandez-Vall et al, 1995].…”