Schwann cells, neurotrophic factors, and peripheral nerve regeneration

Frostick, Simon P.; Yin, Qudong; Kemp, Graham J.

doi:10.1002/(sici)1098-2752(1998)18:7<397::aid-micr2>3.0.co;2-f

Cited by 473 publications

(306 citation statements)

References 79 publications

(77 reference statements)

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“…[30][31][32][33] However, there have been major problems in delivering neurotrophic factors into the nervous system, such as the serum half-life of the recombinant protein may be short and the blood-brain barrier limits access to the central nervous system. This study provided evidence for the beneficial effects of BMP7 gene transfer on the regeneration/protection of the injured sciatic nerves in rats and raised the possibility of developing BMP7 gene transfer as a potential neuroprotective agent for peripheral nerve repair applications.…”

Section: Discussionmentioning

confidence: 99%

Adenoviral gene transfer of bone morphogenetic protein-7 enhances functional recovery after sciatic nerve injury in rats

Pan

et al. 2010

Gene Ther

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Bone morphogenetic proteins (BMPs), members of the transforming growth factor-b subfamily, function as instructive signals for neuronal lineage commitment and promote neuronal differentiation. However, the mechanism of BMP7 action in vivo after peripheral nerve injury is poorly understood. This study examines the efficacy of gene transfer of adenoviral (Ad) BMP7 on peripheral neuropathy. Transgene expression was found in both Ad-infected sciatic nerves and their respective remote neurons, indicating Ad transduction by a retrograde transport. After AdBMP7 infection to nerves, the sciatic nerves were crushed or transected. Hind limb functional behavior, including rotarod test and sciatic functional index, were conducted in rats weekly after nerve injury. Interestingly, enhanced BMP7 expression significantly improved hind limb functional recovery in AdBMP7-transduced rats when compared with AdGFPtransduced nerve-crushed or transected rats. Furthermore, AdBMP7 transduction reduced injury-induced macrophage activation, nerve demyelination and axonal degeneration. By contrast, AdBMP7 infection did not affect the hyperalgesia paw-withdrawal latency after nerve injury. We further examined the effect of AdBMP7 infection on sciatic nerve explant and Schwann cell cultures. Enhanced cell proliferation was significantly increased by AdBMP7 transduction in both cultures. Taken together, BMP7 overexpression by Ad gene transfer was beneficial in both nerves and Schwann cells on functional recovery after sciatic nerve injury in rats.

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Section: Discussionmentioning

confidence: 99%

Adenoviral gene transfer of bone morphogenetic protein-7 enhances functional recovery after sciatic nerve injury in rats

Pan

et al. 2010

Gene Ther

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“…In less severe nerve injuries, this may prevent regenerating axons from growing into epineurial tissue and thereby guide them toward the distal endoneurial tubes. There, Schwann cells produce a wide range of neurotrophic factors (Frostick et al, 1998), eventually leading to successful regeneration. The neuroma material used in this study, however, was obtained from patients without clinical signs of spontaneous functional recovery.…”

Section: Discussionmentioning

confidence: 99%

Human Neuroma Contains Increased Levels of Semaphorin 3A, Which Surrounds Nerve Fibers and Reduces Neurite ExtensionIn Vitro

Tannemaat

Korecka²,

Ehlert³

et al. 2007

J. Neurosci.

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Neuroma formation after peripheral nerve injury is detrimental to functional recovery and is therefore a significant clinical problem. The molecular basis for this phenomenon is not fully understood. Here, we show that the expression of the chemorepulsive protein semaphorin 3A (sema3A), but not semaphorin 3F, is increased in human neuroma tissue that has formed in severe obstetric brachial plexus lesions. Sema3A is produced by fibroblasts in the epineurial space and appears to be secreted into the extracellular matrix. It surrounds fascicles, minifascicles, or single axons, suggesting a role in fasciculation and inhibition of neurite outgrowth. Lentiviral vector-mediated knock-down of Neuropilin 1, the receptor for sema3A, leads to increased neurite outgrowth of F11 cells cultured on neuroma tissue, but not of F11 cells cultured on normal nerve tissue. These findings demonstrate the putative inhibitory role of sema3A in human neuroma tissue. Our observations are the first demonstration of the expression of sema3A in human neural scar tissue and support a role for this protein in the inhibition of axonal regeneration in injured human peripheral nerves. These findings contribute to the understanding of the outgrowth inhibitory properties of neuroma tissue.

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“…Many different cells are studied with gold and carbon implants, particularly osteoblastic ones [2,[7][8][9]. On the other hand, neural cell studies are relatively at their early stages and mostly they are studied for peripheral neural therapies as tube and cuff forms [10][11][12][13][14]. In a study which was performed by Bieberich et al, C and Au electrodes are located in PC12 cell culture which is a model cell line for neural differentiation and it is reported that both synapsis formation and neural differentiation were observed on Au side [15].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Carbon and Gold Ion Implanted Surfaces on Neuronal Stem Cells’ Functions

Sokullu¹,

DaÄcÄ²,

Gözen³

et al. 2017

J Biotechnol Biomater

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Biomaterials have been used in medicine for decades to improve the functions of tissues and organs. They are also used as prosthesis and implants which are designed to substitute functions of a lacking organ or tissue. Carbon (C) and Gold (Au) were particularly chosen due to their biocompatibility and applied as implants for decades. Carbon and gold were great ion sources for medical applications, as well. In this study, polystyrene dishes were modified using gold and carbon ions via ion implantation technique. Using this surface modification method, it was aimed to improve surface characteristics and achieve a bioactive surface for neural stem cells. Even though the integration of stem cells was promising, neural stem cell studies still have many milestones to reach. Neuro-regeneration was the most desired function for people who suffer from neural system diseases. Changing surface characteristics of scaffolds was a way to promote regeneration and ion implantation was one of the methods to modify surface properties which play a huge role in enhancing the proliferation and integration of cells. In this study, it was observed that the ion implantation stimulated the neural proliferation and the implantation of different ions on cell culture surfaces was essential to determine the effects of this technique on adhesion, proliferation, differentiation and apoptosis properties of cells in details.

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Schwann cells, neurotrophic factors, and peripheral nerve regeneration

Cited by 473 publications

References 79 publications

Adenoviral gene transfer of bone morphogenetic protein-7 enhances functional recovery after sciatic nerve injury in rats

Adenoviral gene transfer of bone morphogenetic protein-7 enhances functional recovery after sciatic nerve injury in rats

Human Neuroma Contains Increased Levels of Semaphorin 3A, Which Surrounds Nerve Fibers and Reduces Neurite ExtensionIn Vitro

The Effects of Carbon and Gold Ion Implanted Surfaces on Neuronal Stem Cells’ Functions

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