1997
DOI: 10.1111/j.1460-9568.1997.tb01396.x
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Schwann Cells Modify Expression of Acetylcholinesterase and Butyrylcholinesterase at Rat Neuromuscular Junctions

Abstract: Using a monoclonal antibody (6.17) directed against a Schwann antigen, we have shown that Schwann cells synthesize a molecule implicated in a change of expression of synaptic cholinesterases, AChE and BChE, during muscle differentiation. In vitro, during synaptogenesis, the two enzymes are first present at developing synapses, and addition of Schwann cells to muscle-neuron co-cultures induces a disappearance of BChE, leaving only AChE activity as in the adult neuromuscular junction. This effect is inhibited by… Show more

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Cited by 10 publications
(11 citation statements)
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“…A large fraction of OLN-93 cells also present significant levels of AChE immunoreactivity (not shown). Schwann cells at the neuromuscular junction also express both types of cholinesterases (Chapron et al, 1997). After antisense BChE transfection, BChE gene expression was efficiently lowered, as shown by immunoreactivity and Northern blots (not shown).…”
Section: Bche In Oln-93 Cells Acts By a Nonenzymatic Mechanismmentioning
confidence: 93%
See 1 more Smart Citation
“…A large fraction of OLN-93 cells also present significant levels of AChE immunoreactivity (not shown). Schwann cells at the neuromuscular junction also express both types of cholinesterases (Chapron et al, 1997). After antisense BChE transfection, BChE gene expression was efficiently lowered, as shown by immunoreactivity and Northern blots (not shown).…”
Section: Bche In Oln-93 Cells Acts By a Nonenzymatic Mechanismmentioning
confidence: 93%
“…For example, the enzyme is found accumulated at the blood-brain barrier interface (Joo and Csillik, 1966;Ciani, 1984;Risau and Wolburg, 1990), most likely due to its production by astroglial cells. In primary Schwann cell cultures of the chick sciatic nerve, BChE is expressed at significant levels (Weiss et al, 1996;Chapron et al, 1997). Moreover, in Mü ller cells of the developing chick retina, BChE expression is spatiotemporally regulated (Reiss et al, 1997).…”
Section: Introductionmentioning
confidence: 99%
“…This method allows the visualization of AChE patches as a stable signal and is therefore more adapted than immunofluorescence against AChE to measure the length of a large number of AChE patches. In neuron–myotube cocultures, synaptic patches of cholinesterase revealed a much stronger AChE than butyrylcholinesterase activity (Chapron et al ., 1997). Therefore, synaptic cholinesterase patches were composed largely of AChE forms, with very few butyrylcholinesterase forms, and we chose to name them AChE patches.…”
Section: Methodsmentioning
confidence: 99%
“…A growing body of evidence suggests that synaptic glia, which include both TSCs that cover peripheral synapses and astrocytes that cover central synapses, are intimately involved in virtually every facet of synapses, from their genesis and differentiation to their long-term maintenance and function [Ullian et al, 2001;Koenig et al, 1998;Robitaille, 1998;Chapron et al, 1997;Trachtenberg and Thompson, 1997]. For example, recent evidence indicates that TSCs in mature muscle respond to denervation by extending processes which provide a substrate that promotes axonal reinnervation of denervated endplates [Reynolds and Wolf, 1992;Son and Thompson, 1995a, b;O'Malley et al, 1999;Love and Thompson, 1999;Koirala et al;.…”
Section: Introductionmentioning
confidence: 99%