Schwann Cell Seeded Guidance Tubes Restore Erectile Function After Ablation of Cavernous Nerves in Rats

Maÿ, F.; Weidner, Norbert; Matiasek, Kaspar; Caspers, C.; Mrva, Thomas; Vroemen, Maurice; Henke, Julia; Lehmer, A.; Schwaibold, Hartwig; Erhardt, W.; Gänsbacher, Bernd; Härtung, R.

doi:10.1097/01.ju.0000132357.05513.5f

Cited by 40 publications

(34 citation statements)

References 15 publications

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“…Endogenous SCs produce various neurotrophic factors, cytokines, and extracellular matrix molecules, thereby providing structural support and guidance for regenerating axons [6]. Autologous SC transplantation has shown promising clinical results, such as in remyelinating damaged axons and restoring their electrical conduction properties [7]. However, to obtain SCs from nerve biopsies, another functional nerve must be sacrificed for the in vitro expansion of the desired cell line.…”

Section: Introductionmentioning

confidence: 99%

Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration

Jung

Park

Choi

et al. 2016

IJMS

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Schwann cells (SCs), which produce neurotropic factors and adhesive molecules, have been reported previously to contribute to structural support and guidance during axonal regeneration; therefore, they are potentially a crucial target in the restoration of injured nervous tissues. Autologous SC transplantation has been performed and has shown promising clinical results for treating nerve injuries and donor site morbidity, and insufficient production of the cells have been considered as a major issue. Here, we performed differentiation of tonsil-derived mesenchymal stem cells (T-MSCs) into SC-like cells (T-MSC-SCs), to evaluate T-MSC-SCs as an alternative to SCs. Using SC markers such as CAD19, GFAP, MBP, NGFR, S100B, and KROX20 during quantitative real-time PCR we detected the upregulation of NGFR, S100B, and KROX20 and the downregulation of CAD19 and MBP at the fully differentiated stage. Furthermore, we found myelination of axons when differentiated SCs were cocultured with mouse dorsal root ganglion neurons. The application of T-MSC-SCs to a mouse model of sciatic nerve injury produced marked improvements in gait and promoted regeneration of damaged nerves. Thus, the transplantation of human T-MSCs might be suitable for assisting in peripheral nerve regeneration.

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Section: Introductionmentioning

confidence: 99%

Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration

Jung

Park

Choi

et al. 2016

IJMS

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“…These conduits also serve to introduce neurotrophic factors or cells that may enhance nerve regeneration. Investigations thus far have been conducted in rats, showing potential for autologous genitofemoral nerve [41,42], amniotic membrane [23], silicone nerve tubes [24], and Schwann cell-seeded nerve guidance tubes [43]. Preclinical advances in this area have largely served to promote peripheral nerve grafting of resected or inadvertently damaged cavernous nerves in men undergoing radical prostatectomy [44,45].…”

Section: Nerve Guidesmentioning

confidence: 98%

Neuromodulatory therapy with applications for the radical pelvic surgery patient

Burnett

2005

Current Sexual Health Reports

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“…No obstante, un interesante estudio piloto experimental realizado por May, en Munich, abrió una nueva vía para la reconstrucción nerviosa mediante ingeniería tisular de nervios cavernosos 32 . Se expandieron en cultivos células de Schwann aisladas de nervios ciáticos de rata y se sembraron en tubos de silicona que se interpusieron microquirúrgicamente en resecciones bilaterales de 5 mm de nervios cavernosos en ratas.…”

Section: Nervios Cavernososunclassified

Medicina regenerativa en Andrología: Ingeniería Tisular y terapia génica como posibles opciones de tratamiento para las deformaciones peneanas y la disfunción eréctil

Schultheiss¹,

Jonas²

2006

Actas Urol Esp

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RESUMENMEDICINA REGENERATIVA EN ANDROLOGÍA: INGENIERÍA TISULAR Y TERAPIA GÉNICA COMO POSIBLES OPCIONES DE TRATAMIENTO PARA LAS DEFORMACIONES PENEANAS Y LA DISFUNCIÓN ERÉCTIL Se están investigando la ingeniería tisular y la terapia génica en estudios en animales para reconstruir el tejido peneano o tratar la disfunción eréctil. En esta revisión se pretende examinar estos esfuerzos experimentales de los últimos años y se intenta ofrecer una breve introducción a la metodología básica de estas nuevas técnicas del campo de la medicina regenerativa. ABSTRACT REGENERATIVE MEDICINE IN ANDROLOGY: TISSUE ENGINEERING AND GENE THERAPY AS POTENTIAL TREATMENT OPTIONS FOR PENILE DEFORMATIONS AND ERECTILE DYSFUNCTIONTissue engineering and gene therapy are currently investigated in animal studies for reconstructing penile tissue or treating erectile dysfunction. This review aims to ecamine these experimental efforts from the last years and tries to give a brief introduction to the basic methodology of these new techniques from the field of regenerative medicine.

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Schwann Cell Seeded Guidance Tubes Restore Erectile Function After Ablation of Cavernous Nerves in Rats

Abstract: Schwann cell seeded guidance tubes restore erectile function after the ablation of cavernous nerves in rats and they are superior to autologous nerve grafts.

Cited by 40 publications

References 15 publications

Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration

Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration

Neuromodulatory therapy with applications for the radical pelvic surgery patient

Medicina regenerativa en Andrología: Ingeniería Tisular y terapia génica como posibles opciones de tratamiento para las deformaciones peneanas y la disfunción eréctil

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