Schnellnachweis von Mikroorganismen in flüssigen Lebensmitteln durch Bestimmung von Adenosintriphosphat mit einem Biolumineszenz-Verfahren

Baumgart, J.; Fricke, K. J.

doi:10.1007/978-3-322-88128-1

Cited by 3 publications

(6 citation statements)

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“…In a recent review on application of ATP measurements to the food industry, Stannard and Gibbs (1986) found that most authors reported sensitivities in the range of 10' organisms/ml; these authors concluded that this level of detection would be appropriate only for highly contaminated samples. However, with liquid samples and the addition of a filtration step, Baumgart et al (1980) detected lo" bacterialmi when a litre of mineral water was filtered. Lonvaud-Funel and Joyeux (1982) detected 10' bacteria/ml of wine by combining a filtration step and an incubation prior to ATP measurement.…”

Section: Discussionmentioning

confidence: 99%

Determination of sterilization effectiveness by measuring bacterial growth in a biological indicator through firefly luciferase determination of ATP

Webster

Walker

Ford

et al. 1988

J. Biolumin. Chemilumin.

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A bioluminescence procedure for measurement of microbial ATP allows a rapid determination of the effectiveness of autoclave sterilization. This determination is achieved faster than detection of acid production in a biological indicator via a pH indicator. Bacterial outgrowth from spores on test strips of the biological indicator was detected by measurement of ATP using the firefly luciferase reaction. A measureable increase in ATP was found after 5 hours of incubation of a biological indicator that had been treated under sterilizing conditions that produced 75% sterility of the biological indicator as measured by acid production. This is a marked improvement over the 24-48 hours of incubation currently required.

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Section: Discussionmentioning

confidence: 99%

Determination of sterilization effectiveness by measuring bacterial growth in a biological indicator through firefly luciferase determination of ATP

Webster

Walker

Ford

et al. 1988

J. Biolumin. Chemilumin.

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“…A correlation of only 50% was obtained between ATP and colony count results but the agreement with microscopic count was rather better (83%). Baumgart et al, (1980b) attempted a similar approach but obtained ATP/cfu values 10-to 100-fold higher than expected, particularly at lower levels of contamination (<lo7 cfu/ml). These authors had an equal lack of success when a cation-exchange resin was used to adsorb micro-organisms from meat homogenates.…”

Section: Samples Containing a Mixture Of Microbial And Somatic Cellsmentioning

confidence: 98%

“…For example, D' Eustachio and showed that, for thirteen species of aerobic bacteria, the mean value of ATP/cfu was 0.47 fg (1 femtogram (fg) = g), with actual values ranging between 0.22 and 1.03 fg, and that there was a linear relationship between ATP and colony count. Yeast species in general have been shown to contain at least 100-fold as much ATP as bacteria (Baumgart et al, 1980a;Sharpe et ul., 1970).…”

Section: Atp Assay To Estimate Micro-organismsmentioning

confidence: 99%

Rapid microbiology: Applications of bioluminescence in the food industry a review

Stannard

Gibbs

1986

J. Biolumin. Chemilumin.

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The bioluminescent assay of ATP is rapid and simple and may be used as an estimate of microbial numbers. It therefore shows great potential as a technique to provide information on the microbiological quality of a food within a few minutes, in comparison with conventional techniques, which provide results retrospectively. However, despite the advantages of speed and sensitivity, no food microbiologists are using the technique for routine quality control and hygiene monitoring. This review seeks to highlight the reasons for this, and to offer some ideas for future research to increase the acceptance of ATP assays within the food industry.

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“…Simple filtration procedures are then sufficient (58). However, since most products contain high contents of somatic ATP, often exceeding microbial ATP levels, pretreatments are necessary.…”

Section: Applications Of the Atp Assaymentioning

confidence: 99%

“…Estimation of the microbial load on meat by the ATP bioluminescence assay is also hampered by interfering somatic ATP (68). The ATP background levels in meat samples, for example, may be equivalent to 10 5 to 10 s CFU/g (based on an ATP content of 1 fg/CFU).…”

Section: Meat and Meat Productsmentioning

confidence: 99%

Changing perspectives in food microbiology: Implementation of rapid microbiological analyses in modern food processing

Veld

Hartog

Hofstra

1988

Food Reviews International

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This review article summarizes the perspective for the introduction of novel microbiological techniques into modern food manufacturing and processing. Changes are discussed that have occurred recently in the microbiological control of food operations (process microbiology)-for example, the "hazard analysis critical control point" (HACCP) concept, which can be elaborated to a longitudinal integrated chain assessment by monitoring critical control points. In addition, we describe the recent developments in rapid analytical methodology that can be implemented under these concepts. These techniques are based on two major approaches; that is, they are either aimed at estimating the total microbial load as a hygiene parameter, or at detecting and enumerating specific pathogenic or toxigenic microorganisms and their metabolites. We conclude that, at this moment, several of these methods are significant improvements compared with the conventional techniques. However their use in preventive quality assurance by integrated chain assessment, as well as in routine food (end product) control, has been limited to certain areas of the food industries as yet.

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Schnellnachweis von Mikroorganismen in flüssigen Lebensmitteln durch Bestimmung von Adenosintriphosphat mit einem Biolumineszenz-Verfahren

Cited by 3 publications

References 0 publications

Determination of sterilization effectiveness by measuring bacterial growth in a biological indicator through firefly luciferase determination of ATP

Determination of sterilization effectiveness by measuring bacterial growth in a biological indicator through firefly luciferase determination of ATP

Rapid microbiology: Applications of bioluminescence in the food industry a review

Changing perspectives in food microbiology: Implementation of rapid microbiological analyses in modern food processing

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