Schnellmarkierte Polyphosphate und Metaphosphate bei der Blaualge Anacystis nidulans

Niemeyer, R.; Richter, Gerhard

doi:10.1007/bf00408563

Cited by 30 publications

(9 citation statements)

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“…The rate of transport is related to the sizes of the internal and external pools of phosphate (10,12,14,22). Phosphate that is taken up by phosphate-starved cells moves rapidly into polyphosphate (11,19).…”

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confidence: 99%

Phosphate transport and arsenate resistance in the cyanobacterium Anabaena variabilis

Thiel

1988

J Bacteriol

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Cells of the cyanobacterium Anabaena variabiis starved for phosphate for 3 days took up phosphate at about 100 times the rate of unstarved cells. Kinetic data suggested that a new transport system had been induced by starvation for phosphate. The inducible phosphate transport system was quickly repressed by addition of Pi.Phosphate-starved cells were more sensitive to the toxic effects of arsenate than were unstarved cells, but phosphate could alleviate some of the toxicity. Arsenate was a noncompetitive inhibitor of phosphate transport; however, the apparent Ki values were high, particularly for phosphate-replete cells. Preincubation of phosphate-starved cells with arsenate caused subsequent inhibition of phosphate transport, suggesting that intracellular arsenate inhibited phosphate transport. This effect was not seen in phosphate-replete cells.

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confidence: 99%

Phosphate transport and arsenate resistance in the cyanobacterium Anabaena variabilis

Thiel

1988

J Bacteriol

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“…Only cultures of low density (less than 10' cells per ml) can be used if logarithmic growth is to be maintained, and most 32P-orthophosphate incorporated by the cells appears in heterogeneous, ribonucleaseand deoxyribonuclease-insensitive material which copurifies with RNA and migrates between 23S rRNA and transfer RNA on polyacrylamide gels. We believe this material to be polyphosphate (13). That fraction of this material which comigrates with 5S rRNA on gels can be removed from the RNA on small preparative MAK (methylated-albumin kieselguhr) columns as described (15).…”

Section: Methodsmentioning

confidence: 99%

Precursor of 5 S Ribosomal Ribonucleic Acid in the Blue-Green Alga Anacystis nidulans

1974

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The maturation of 5S ribosomal ribonucleic acid (rRNA) in the obligately photoautotrophic unicellular blue-green alga Anacystis nidulans has been studied by using polyacrylamide gel electrophoresis and Ti ribonuclease oligonucleotide analysis. A. nidulans mature 5S rRNA (m5) is of approximately the same molecular weight as the 5S rRNA of Escherichia coli, and is derived by cleavage of a precursor (p5) containing a few (three to six) additional nucleotides. Some of these additional nucleotides occur at the 5' end of the precursor molecule; others may occur at the 3' end. Kinetic experiments indicate that precursors of mature 5S rRNA larger than p5 either do not exist or are very transient in A. nidulans. These results are discussed in relation to those obtained with other prokaryotes.

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“…Cyclic compounds (n = 3-8) have been found in different algae (Niemeyer and Richter 1969;Niemeyer 1976) and higher plant species (Niemeyer 1975).…”

Section: Chemical Synthesismentioning

confidence: 99%

Methods for Investigation of Inorganic Polyphosphates and Polyphosphate-Metabolizing Enzymes

Lorenz¹,

Schröder²

1999

Inorganic Polyphosphates

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One of the main reasons for the fact that inorganic polyphosphates were a neglected area of research over a long period of time was the lack of reliable experimental tools for the study of these polymers. In recent years, the range and specificity of the methods available have been revolutionized by the development of novel techniques especially in the field of molecular biology and their application to the investigation of polyphosphates. In particular, the availability of specific polyphosphate-dependent enzymes has been a considerable progress for the development of definite and exact methods. However, for certain questions, optimal methods are still missing.The aim of this chapter is to summarize and to illustrate the most important and most widely used methods for investigation of polyphosphates, with their advantages and disadvantages. The efficiency and applicability of nuclear magnetic resonance spectroscopy for studying polyphosphates is described in Chapter 13. Furthermore, novel promising methods for the isolation and quantification of polyphosphate as well as for the measurement of polyphosphate-dependent enzyme activities by using the power of specific enzymes are explained and documented in Chapter 12. 2 Preparation of Inorganic Polyphosphates Chemical SynthesisFormally, inorganic polyphosphates are formed by condensation of orthophosphate under formation of phospho anhydride bonds. Three different groups of basic structures can result: linear, branched and cyclic molecules. The latter are also called metaphosphates. When orthophosphoric acid or its salts are heated these substances form condensates by leaving of water.

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Schnellmarkierte Polyphosphate und Metaphosphate bei der Blaualge Anacystis nidulans

Cited by 30 publications

References 9 publications

Phosphate transport and arsenate resistance in the cyanobacterium Anabaena variabilis

Phosphate transport and arsenate resistance in the cyanobacterium Anabaena variabilis

Precursor of 5 S Ribosomal Ribonucleic Acid in the Blue-Green Alga Anacystis nidulans

Methods for Investigation of Inorganic Polyphosphates and Polyphosphate-Metabolizing Enzymes

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