Schleichera oleosa Seed Extract Reduced the Proliferation of Breast Cancer by Regulating the BRCA1 and p16 Genes

Abstract

Background: Breast cancer is the most commonly diagnosed cancer and the leading cause of cancer death in females worldwide. Schleichera oleosa (kusum tree) belongs to the Sapindaceae family commonly found in many states of India. This plant is traditionally being used in various pathological conditions. Methods: In vitro studies were performed using seed extract of Schleichera oleosa. Different concentrations of seed extracts were treated on MCF-7 breast cancer cell line and its effect on migration and colony formation were observed. BRCA1 and p16 gene expression was analyzed by real-time PCR and Western blotting. Results: We have analyzed anticancer and anti-metastatic effects of seed extract in breast cancer and IC 50 was 140µg/ml concentration. Further, its inhibitory role in cell migration and colony formation was at 140µg/ml (P<0.0001) concentration and reduced significantly growth of sphere at 140 µg (P<0.0031) and 150µg (P<0.0010) concentration after 5 days of treatment. The apoptosis study was shown a significant increase at 140 µg (P<0.0001) in apoptotic cells. Expression of BRCA1 and p16 were found to be over-expressed as 1.4 and 1.7 fold, respectively, at 140µg/ml concentration after 24 h of treatment at the transcription level. BRCA1 protein was up-regulated but p16 expression down-regulated at 140 to 150µg/ml (One-Way ANOVA, P<0.0001) concentration. Conclusion: In this study, we found a significant role of S. Oleosa seed extract has an anti-cancer as well as anti-metastatic via up-regulation of BRCA1 and p16 genes in breast cancer cells.

Role of identified proteins in the proteome profiles of CDK4/6 inhibitor-resistant breast cancer cell lines

“…Colony formation assay was used to evaluate the drug-resistance characteristics via evaluation of cell survival and proliferation indicated by cell survival, the number of colonies formed and their growth. 22 As shown in Fig. 2A and B, very few number of colonies were formed in Ab/Pb drug-treated sensitive MCF-7 cells as compared to the control.…”

“…Here we have studied the tendency of cell migration, wound healing and proliferation using the wound healing assay. 22 The results show that the gap made at 0 h in MCF-7 with the Ab/Pb drug as compared to MCF-7 without Ab/Pb drug treatment is not filled or slightly filled by cells at 12, 24 and 36 h indicating the high efficacy of drugs in the sensitive cells. However, the gaps made at 0 h in Ab-R-MCF-7 with the Ab/Pb drug as compared to Ab/Pb-R-MCF7 without the drug are significantly filled and almost the same under both conditions.…”